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DC negative regulation factor targeting silencing and DC mobilized amplification combined tumor therapy method

A negative regulatory factor, targeted silencing technology, applied in the field of biomedicine, can solve the problems of affecting the effect of siRNA-DC vaccine, lack of cell introduction system of gene silencing technology, and inability to target the intervention of immunosuppressive molecules IDO, and achieve tumor suppression. Cell growth, wide application range, effect with few side effects

Inactive Publication Date: 2018-06-08
NANCHANG UNIV
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Problems solved by technology

siRNA is currently the most effective technology to selectively block gene expression, but siRNA-mediated gene silencing technology currently lacks an effective and specific cell delivery system, which not only affects the effect of siRNA-DC vaccine in vitro, but also fails to immune DC in vivo. Inhibition molecule IDO for targeted intervention

Method used

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  • DC negative regulation factor targeting silencing and DC mobilized amplification combined tumor therapy method
  • DC negative regulation factor targeting silencing and DC mobilized amplification combined tumor therapy method
  • DC negative regulation factor targeting silencing and DC mobilized amplification combined tumor therapy method

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Embodiment 1

[0028] Example 1: In vivo mobilization of DC expansion and targeted silencing of IDO expression in DC combined therapy of mouse lung cancer model

[0029] figure 1 Schematic diagram of the new tumor immune combination therapy technology for mobilizing and expanding DCs and targeting the expression of immune negative regulators in DCs in the present invention.

[0030] 1. Synthesis of man-GNR-siRNA nano-delivery system targeting DC transport in vivo:

[0031] (1) Chemical synthesis of GNR: Gold nanorod GNR was synthesized according to the chemical reduction method, and the TEM characterization results are as follows figure 2 As shown in A;

[0032] (2) Chemical synthesis of MUA-PEI / MUA-PEI-mannose: According to the condensation acylation reaction, in HCl 3 Prepare MUA-PEI in a solvent; prepare MUA-PEI-mannose in a DMSO solvent;

[0033](3) Synthesis of man-GNR: According to the bonding between -SH and Au, the surface of GNR was modified with MUA-PEI and MUA-PEI-mannose in ...

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Abstract

The invention provides a novel tumor therapy method, and concretely provides a DC (dendritic cell) negative regulation factor targeting silencing and DC mobilized amplification combined tumor therapymethod. The method treats tumors through combining DC in-vivo mobilized amplification with DC immune negative regulation factor expression miRNA targeting tumor targeting silencing; and DCs are introduced into miRNA in the DCs through a DC nanometer delivery system after undergoing in-vivo mobilized amplification, and the expression of the immune negative regulation factor in the DCs is controlledthrough an RNA interference technology, so the immunization capability of the immunization system to tumors is further enhanced. The therapy method can be used as an auxiliary therapy method for operations, radiotherapy, chemotherapy or other clinic therapy means, and also can be used to treat solid tumors or nonsolid tumors; and the therapy method in the invention has the advantages of remarkable effect, small side effects, effectiveness in inhibiting and even killing tumor cells, and provision of a new way for the immune therapy research of clinic tumors.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a novel tumor therapy that combines the expression of negative regulatory factors targeting silencing DC and the mobilization and expansion of DC in vivo. Background technique [0002] Tumor has always been an urgent problem to be solved in the medical field. The occurrence of tumor involves a variety of molecular and gene changes, including the mutation and amplification of proto-oncogenes or the loss and inactivation of tumor suppressor genes and other factors. [0003] Tumor immunotherapy is the concept of stimulating and strengthening the body to achieve the purpose of controlling and killing tumor cells. It is often used as an adjuvant therapy in combination with conventional therapies such as surgery, chemotherapy or radiotherapy. Among them, dendritic cells (DC) are the only full-time antigen-presenting cells (Antigenpresenting cells, APCs) known to activate naive T ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61K31/7105A61K48/00A61P35/00
CPCA61K31/7105A61K38/18A61K2300/00
Inventor 张瑜娟闵卫平付佳敏彭珊珊
Owner NANCHANG UNIV
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