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Method for separating and purifying steviol glycoside monomers

A technology of stevioside and stevioside, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of low extraction purity, inability to achieve continuous production, etc., and achieve high purity, high yield, solvent low cost effect

Inactive Publication Date: 2018-06-08
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

People such as Zhenbin Chen use macroporous adsorption resin to purify stevioside, adopt the mixed resin of LZ-1+LZ-20+LZ-30+LZ-37+LZ-36, the mixing ratio is m(LZ-1):m (LZ-20):m(LZ-30):m(LZ-37):m(LZ-36)=2:3:1:3:2, other operating parameters are set to C 0 =0.416mg / mL, V=50mL, pH=7, T=65℃, E=85%, t a =90min,t d =120min, it is produced by macroporous adsorption resin, the extraction purity is low, and continuous production cannot be realized ("Preparative separation of Rebaudiana A from commercialized steviolglycosides by macroporous adsorption resins mixed bed"[J], Zhenbin Chen et al., Separation and Purification Technology, 2012,89:22~30)

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  • Method for separating and purifying steviol glycoside monomers
  • Method for separating and purifying steviol glycoside monomers

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Experimental program
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Embodiment 1

[0043] 1. Equipment parameter selection: A three-zone simulated moving bed chromatography system is adopted. The system includes a chromatographic system divided into zone I, zone II and zone III, N=4, and the number of chromatographic columns is 1, 1, and 2 respectively.

[0044] The model of the chromatographic column is 150*10mm, and the stationary phase is amino-bonded silica gel filler 6010-NH 2 , the particle size of the filler is 60.4 μm, and the mobile phase is an aqueous solution of acetonitrile with a volume fraction of acetonitrile of 70%.

[0045] 2. Preparation of stevioside sample solution: mix stevioside with an aqueous solution of acetonitrile with a volume fraction of 70% acetonitrile to obtain a stevioside sample solution with a mass concentration of 0.5 g / L.

[0046] 3. Inject the stevioside sample solution A and the mobile phase B from the sample liquid pump 11 and the eluent pump 12 into the three-zone simulated moving bed chromatography system respectivel...

Embodiment 2

[0057] 1. Equipment parameter selection: A three-zone simulated moving bed chromatography system is adopted. The system includes a chromatographic system divided into zone I, zone II and zone III, N=4, and the number of chromatographic columns is 1, 1, and 2 respectively.

[0058] The model of the chromatographic column is 150*10mm, and the stationary phase is amino-bonded silica gel filler 6010-NH 2 , the particle size of the filler is 60.4 μm, and the mobile phase is an aqueous solution of acetonitrile with a volume fraction of acetonitrile of 80%.

[0059] 4. Preparation of stevioside sample solution: mix stevioside with an aqueous solution of acetonitrile with a volume fraction of 80% acetonitrile to obtain a stevioside sample solution with a mass concentration of 8.2 g / L.

[0060] 5. The stevioside sample liquid and mobile phase are respectively injected into the three-zone simulated moving bed chromatography system by the sample liquid pump 11 and the eluent pump 12, and...

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Abstract

The invention provides a method for separating and purifying steviol glycoside monomers and belongs to the technical field of separation and purification. The method specifically comprises the following steps: mixing steviol glycoside samples with an acetonitrile aqueous solution to obtain a steviol glycoside sample solution, taking an amino filler as a stationary phase, taking the acetonitrile aqueous solution as a mobile phase, and separating the sample solution through a three-zone simulated moving bed chromatography separation system to obtain stevioside and rebaudioside-A. Through the method, the stevioside and the rebaudioside-A separated from steviol glycoside by using three-zone simulated moving bed chromatography separation system are high in purity; meanwhile, the method is simple in separation process, continuous and automatic in process, high in yield and low in solvent consumption, the solvent can be reusable and the clean production can be achieved.

Description

technical field [0001] The invention relates to the technical field of separation and purification, in particular to a method for separating and purifying stevioside monomers. Background technique [0002] Stevioside is a natural sweetener with high sweetness and low calories. Stevioside has a variety of monomers. Baudioside-A (Rebaoudioside-A, RA) and stevioside (Stevioside, ST) are the two most common Monomer, wherein the sweetness of rebaudioside A is 350-450 times that of sucrose, and the sweetness of stevioside is 250-300 times that of sucrose. At the same time, stevioside has stable properties such as heat resistance and anti-corrosion, and can also be added as a sweetener to food, medicine, cosmetics, etc. to increase its shelf life. In addition, according to a large number of toxicological studies, stevioside is easily digested and excreted in the human body. At the same time, other studies have shown that it can also lower blood pressure, blood sugar, cholesterol a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/256C07H1/06
CPCC07H1/06C07H15/256
Inventor 袁阳平姚传义卢英华
Owner XIAMEN UNIV