Anti-aging composition comprising carnosine, soy peptide, and andrographis paniculata extract

Abstract

The anti-aging cosmetic composition comprising carnosine, a soy peptide, and an Andrographis paniculata extract as active ingredients according to the present invention is not only capable of reducingskin aging effectively by having the effects of inhibiting cellular aging caused by oxidative stress, increasing skin-activating growth factor responsiveness, and further enhancing DEJ and an ECM, but also expected to be usable as an anti-aging cosmetic composition and food composition by being nontoxic to cells.

Description

technical field

[0001] The present invention relates to an anti-aging composition comprising carnosine, soybean peptide and andrographis paniculata extract, more particularly to an anti-aging cosmetic composition or food composition comprising carnosine, soybean peptide and andrographis paniculata extract , the anti-aging cosmetic composition or food composition exhibits the effect of enhancing the protein at the junction of the dermis and the epidermis (DEJ: Dermal epidermal junction), the enhancement effect of the extracellular matrix (ECM: Extracellular matrix), and the suppression of oxidative stress caused by The effect of cell aging induced and the effect of enhancing the responsiveness of active growth factors of the skin. Background technique

[0002] As the skin is the first organ to show aging, a lot of research has been done in the field of cosmetics in order to delay or prevent skin aging. Usually, skin aging begins around the age of 25, and skin aging really be...

Examples

preparation example 1

[0041] prepare Andrographis paniculata extract

[0042] After the whole herb of Andrographis paniculata was cleaned, it was dried in the shade and ground, then the dry sample of 1kg and the auxiliary solvent ethanol (99.5%) of 500~700ml were put into a supercritical fluid extraction reactor (Natex, Australia), and in the reaction The temperature inside the vessel was 40° C., the pressure was 10 MPa, and the S / F ratio (supercritical fluid kg / Feed kg) was 35, and the extraction was performed using a total amount of 40 kg of carbon dioxide. Then, the mixture of carbon dioxide, ethanol and raw material is passed through the separation device, and the extract is separated from the carbon dioxide by changing the pressure and temperature of the mixture. The obtained extract was concentrated under reduced pressure to remove ethanol in the obtained extract to prepare 121.4 g of Andrographis paniculata extract.

preparation example 2

[0043] Manufacture of a composition containing carnosine, andrographis paniculata extract and soybean peptide

[0044] Carnosine, Andrographis paniculata extract and soybean peptide were mixed at a weight ratio of 1:0.5:1 to prepare a composite compound (AC) composition containing carnosine, Andrographis paniculata extract and soybean peptide.

Embodiment 1

[0045] Cytotoxicity test of the composition

[0046] A cytotoxicity test (MT T) of the composition was performed using human-derived fibroblasts. The fibroblasts were treated with carnosine, andrographis paniculata extract, and soybean peptide at concentrations of 0, 10, 25, and 50 μg / ml, respectively. After two days of culture, 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT: 3-(4,5-dimetylthiazol-2-yl) 2,5-diphenyltetrazoli umbromide) was used to measure the cytotoxic effect of cells. The cell viability was converted by the percentage relative to the negative control group, and the results are shown in Table 1.

[0047] Table 1

[0048]

[0049] No significant changes were shown in cell number. When each substance was treated together at the maximum concentration that did not show cytotoxicity when treated separately, no significant change in cell viability was observed. Based on this result, the concentration of the complex compound treatment perfo...

technical field

[0001] The present invention relates to an anti-aging composition comprising carnosine, soybean peptide and andrographis paniculata extract, more particularly to an anti-aging cosmetic composition or food composition comprising carnosine, soybean peptide and andrographis paniculata extract , the anti-aging cosmetic composition or food composition exhibits the effect of enhancing the protein at the junction of the dermis and the epidermis (DEJ: Dermal epidermal junction), the enhancement effect of the extracellular matrix (ECM: Extracellular matrix), and the suppression of oxidative stress caused by The effect of cell aging induced and the effect of enhancing the responsiveness of active growth factors of the skin. Background technique

[0002] As the skin is the first organ to show aging, a lot of research has been done in the field of cosmetics in order to delay or prevent skin aging. Usually, skin aging begins around the age of 25, and skin aging really be...

Examples

preparation example 1

[0041] prepare Andrographis paniculata extract

[0042] After the whole herb of Andrographis paniculata was cleaned, it was dried in the shade and ground, then the dry sample of 1kg and the auxiliary solvent ethanol (99.5%) of 500~700ml were put into a supercritical fluid extraction reactor (Natex, Australia), and in the reaction The temperature inside the vessel was 40° C., the pressure was 10 MPa, and the S / F ratio (supercritical fluid kg / Feed kg) was 35, and the extraction was performed using a total amount of 40 kg of carbon dioxide. Then, the mixture of carbon dioxide, ethanol and raw material is passed through the separation device, and the extract is separated from the carbon dioxide by changing the pressure and temperature of the mixture. The obtained extract was concentrated under reduced pressure to remove ethanol in the obtained extract to prepare 121.4 g of Andrographis paniculata extract.

preparation example 2

[0043] Manufacture of a composition containing carnosine, andrographis paniculata extract and soybean peptide

[0044] Carnosine, Andrographis paniculata extract and soybean peptide were mixed at a weight ratio of 1:0.5:1 to prepare a composite compound (AC) composition containing carnosine, Andrographis paniculata extract and soybean peptide.

Embodiment 1

[0045] Cytotoxicity test of the composition

[0046] A cytotoxicity test (MT T) of the composition was performed using human-derived fibroblasts. The fibroblasts were treated with carnosine, andrographis paniculata extract, and soybean peptide at concentrations of 0, 10, 25, and 50 μg / ml, respectively. After two days of culture, 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT: 3-(4,5-dimetylthiazol-2-yl) 2,5-diphenyltetrazoli umbromide) was used to measure the cytotoxic effect of cells. The cell viability was converted by the percentage relative to the negative control group, and the results are shown in Table 1.

[0047] Table 1

[0048]

[0049] No significant changes were shown in cell number. When each substance was treated together at the maximum concentration that did not show cytotoxicity when treated separately, no significant change in cell viability was observed. Based on this result, the concentration of the complex compound treatment perfo...

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