Tissue culture method for increasing survival rate of seed seedlings of millettia speciosa champ

A tissue culture and Niu Dali technology, applied in the field of plant tissue culture, can solve the problems of low survival rate of cutting culture, difficulty in batch culture, low germination rate of seeds, etc., and achieve the effect of maintaining cell vitality, convenient use and rich nutrients

Inactive Publication Date: 2018-06-12
东兰县委荣村伟造林下药材种植合作社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, seed cultivation has high requirements for seeds, and batch collection is difficult. If the germination rate of seeds is low, the loss will be large; the survival rate of cutting cultivation is low, and batch cultivation is difficult.

Method used

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  • Tissue culture method for increasing survival rate of seed seedlings of millettia speciosa champ

Examples

Experimental program
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Effect test

Embodiment 1

[0031]A tissue culture method for improving the survival rate of ox vigorous seedlings, the specific steps are as follows:

[0032] (1) Preparation: 1) Preparation of plant treatment solution: 20 parts of onion extract, 15 parts of Burnet extract, 30 parts of lemon eucalyptus extract, 15 parts of garlic extract, 10 parts of Artemisia annua extract, 10 parts of mugwort The grass extract solution is mixed evenly; 900 parts of absolute ethanol is added, and after mixing evenly, a plant treatment solution with a mass concentration of 10% is obtained;

[0033] Wherein, the preparation of the onion extract comprises the following steps: weighing 30 parts of onions, washing and putting them into a juice extractor, adding 30 parts of water, pressing for 10 minutes, filtering, and concentrating to obtain the onion extract;

[0034] The preparation of the Burnet extract includes the following steps: grind the Burnet, weigh 30 parts of the Burnet powder, put it into a reactor, add 60 par...

Embodiment 2

[0047] A tissue culture method for improving the survival rate of ox vigorous seedlings, the specific steps are as follows:

[0048] (1) Preparation: 1) Preparation of plant treatment solution: 25 parts of onion extract, 20 parts of Burnet extract, 25 parts of lemon eucalyptus extract, 14 parts of garlic extract, 8 parts of Artemisia annua extract, 8 parts of mugwort The grass extract solution is mixed evenly; 800 parts of absolute ethanol is added, and after mixing evenly, a plant treatment solution with a mass concentration of 11% is obtained;

[0049] Wherein, the preparation of the onion extract comprises the following steps: weighing 30 parts of onions, washing and putting them into a juice extractor, adding 35 parts of water, pressing for 15 minutes, filtering, and concentrating to obtain the onion extract;

[0050] The preparation of Burnet extract includes the following steps: grind Burnet, weigh 30 parts of Burnet powder, put it into a reactor, add 70 parts of water, ...

Embodiment 3

[0063] A tissue culture method for improving the survival rate of ox vigorous seedlings, the specific steps are as follows:

[0064] (1) Preparation: 1) Preparation of plant treatment solution: 30 parts of onion extract, 30 parts of Burnet extract, 15 parts of lemon eucalyptus extract, 10 parts of garlic extract, 8 parts of Artemisia annua extract, 7 parts of mugwort The grass extract solution is mixed evenly; 700 parts of absolute ethanol is added, and after mixing evenly, a plant treatment solution with a mass concentration of 12.5% ​​is obtained;

[0065] Wherein, the preparation of the onion extract comprises the following steps: weighing 30 parts of onions, washing and putting them into a juice extractor, adding 40 parts of water, pressing for 18 minutes, filtering, and concentrating to obtain the onion extract;

[0066] The preparation of the Burnet extract includes the following steps: grind the Burnet, weigh 30 parts of the Burnet powder, put it into the reactor, add 8...

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Abstract

The invention belongs to the technical field of plant tissue culture, and discloses a tissue culture method for increasing the survival rate of seed seedlings of millettia speciosa champ. The tissue culture method comprises five steps of sterilization of an explants, primary culture, secondary culture, rooting culture and seedling hardening. According to the tissue culture method disclosed by theinvention, the sterilization of the explants is carried out by adopting a plant treatment solution, chemical reagents such as mercury bichloride are prevented from being used, the environment is protected, and cultured seed seedlings of the millettia speciosa champ are strong in adaptability and higher in survival rate.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a tissue culture method for improving the survival rate of bovine vigorous seedlings. Background technique [0002] Niu Dali is a plant belonging to the family Fabaceae, also known as Zhujiao Li, Admiralty Root, Mountain Lotus Root, Upside Down Admiralty, and Dali Potato. active role. Niu Dali has a long history of drug use. "Herbal Medicinal Preparations" records: "Strengthen tendons and activate collaterals, nourish deficiency and moisten lungs; treat waist and leg pain, rheumatism paralysis, chronic hepatitis, and tuberculosis." "Lu Chuan's Materia Medica" records: "clears the lung and relieves cough, cools and detoxifies, treats hemoptysis, dysentery, febrile disease, body heat, thirst, and dizziness." Since the 1970s, Niu Dali has been used as a raw material for Zhuangyao Jianshen Pills and Qiangli Jianshen Capsules in Chinese patent medicines, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 韦宏明
Owner 东兰县委荣村伟造林下药材种植合作社
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