Method for preparing rabies vaccine for veterinary use

Abstract

The invention discloses a method for preparing rabies vaccine for veterinary use. According to the method, the rabies vaccine for veterinary use is cultivated and produced by a cell bioreactor total-suspension non-serum method. In particular, the method comprises the following steps: performing virus infection on a BHK-21 cell with certain density and adapted to a strain by utilizing a rabies strain PV / BHK-21 and performing virus harvesting for many times, wherein the BHK-21 cell is obtained by performing total-suspension, non-serum and continuous perfusion cultivation by the cell bioreactor;and performing filtering system treatment and deactivation on the harvested virus stock solution, adding an adjuvant to prepare the rabies inactivated vaccine with high immunity and high safety. Through total-suspension and continuous perfusion culture by the cell bioreactor, high-density cell culture can be realized and viruses can be harvested continuously; and through non-serum culture, serum source pollution can be avoided, the purification process can be simplified and allergic reaction of the organism caused by the serum can be reduced. By the method provided by the invention, mass production and popularization can be realized.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a method for cultivating and producing veterinary inactivated rabies vaccine using a cell bioreactor in a fully suspended serum-free manner. Background technique [0002] Rabies is a zoonotic acute infectious disease of the central nervous system caused by Rabies Virus, which was recognized earlier by humans. Rabies virus belongs to ribonucleic acid rhabdovirus, which invades the human or animal body through damaged skin or mucous membranes. Once infected, the mortality rate reaches 100%. Rabies is the infectious disease with the highest mortality rate. About 50,000 people die from rabies every year in the world, 90% of which are in Asia. At present, the proportion of rabies cases in China is still very high, second only to India, and it is one of the countries with severe rabies epidemic in the world. The "Law of the People's Republic of Chin...

AI Technical Summary

Problems solved by technology

The semi-finished antigen produced by the spinner bottle culture method has a low titer, needs to be concentrated, and is labor-intensive, with a long production cycle and a high risk of contamination

Although the microcarrier suspension culture method can effectively proliferate cells, the cost of using microcarriers is high and it is not easy to scale up culture

At present, serum-containing medium is used in the production of domestic rabies vaccines. Because the added serum has problems such as difficult purification, foreign virus carrying, and different quality between batches, the vaccines produced are poor in safety, unstable in quality, and prone to induce allergic reaction

Problems such as low degree of automation, backward production technology, poor antigenicity of virus strains, short maintenance time of production cells, low virus yield, potential allergic reactions of vaccines, and high production costs have seriously affected the development of inactivated rabies vaccines in China