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Gongronella sp. strain and application thereof

A technology of Phytophthora, strains, applied in applications, fungi, chemicals for biological control, etc.

Active Publication Date: 2018-06-12
ANHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there have been no reports of the use of fungi derived from the genus Globulomyces for the promotion of plant growth.

Method used

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  • Gongronella sp. strain and application thereof
  • Gongronella sp. strain and application thereof
  • Gongronella sp. strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 contains the fermentative preparation of ball socket fungal agent of the present invention

[0024] 1. Activation of Glomus w5 strain

[0025] The fungus w5 preserved on the slant was picked onto the CPDA slant, and cultured statically at 28°C for 3 days to activate the strain.

[0026] 2. Preparation and sterilization of medium

[0027] Prepare fermentation medium (15g sucrose, 1.5g yeast extract, 1g KH 2 PO 4 , 0.1g Na 2 PO 4 ·7H 2 O, 0.01g CaCl 2 ), sterilized at 115°C for 20 minutes for later use.

[0028] 3. Fermentation preparation of the spores of the w5 strain of Globula sp.

[0029] Pick 5 pieces of activated mycelia of 4mm gauze w5, inoculate them into sterilized fermentation medium, culture them with vibration at 28°C and 120rpm for 4 days, filter the mycelia with 4 layers of gauze, and collect the fermented liquid at 2400×g The spores were collected by centrifugation under the condition of centrifugal force, and the spores were diluted ...

Embodiment 2

[0030] Embodiment 2 Contains the application of the ball socket fungus agent of the present invention in promoting the growth of kiwifruit plants

[0031] 1. Potted planting of kiwifruit plants

[0032] Take horticultural nutrient soil (purchased from Yufeng Flower Market) and ordinary soil (Nongcui Garden, Anhui Agricultural University) and mix them at a ratio of 1:1. The culture medium was sterilized at 0.14MPa and 120°C for 2 hours for later use.

[0033] The "Hongyang" kiwifruit seeds were sown in cultivation pots filled with sterilized soil, a total of 120 pots. After the kiwi fruit grows 2 pairs of true leaves, the seedlings are thinned and transplanted, and 3-5 seedlings with the same height and uniform growth are kept in each pot.

[0034] 2. The application of the fungus agent w5

[0035] Immediately after transplanting the thinning seedlings, the spore suspension (3 × 10 8 / mL) according to 10mL / strain direct watering treatment 60 pots of kiwifruit plant roots, 6...

Embodiment 3

[0048] Embodiment 3 Contains the application of the ball-shaped fungus agent of the present invention in promoting the disease resistance of kiwifruit plants

[0049] Select 6 pots of kiwifruit plants after 60 days of treatment and 60 days without treatment with the fungus w5 inoculum, select the representative strain JF8 of kiwifruit canker disease activated and cultivated, pick up a single colony of JF8 with a toothpick, and puncture the "Hongyang" kiwifruit leaves and seedlings Between the first pair of true leaves and the third pair of true leaves, as deep as the xylem, clear water was used as a control. After the inoculation, the kiwi fruit was placed in a light incubator at a temperature of 20°C for cultivation; 15 days after the inoculation, the disease was observed. See the experimental results figure 2 , the results showed that the leaves of the plants using the inoculum w5 inoculum were comparable to those of the clear water control group, and no pathogenic microor...

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Abstract

The invention discloses a gongronella sp. strain and application thereof, and belongs to the technical field of microbial application. The strain is a gongronella sp. w5 strain, and the preservation number of the strain is CCTCC: M2017787. Spores of the gongronella sp. w5 strain are prepared into a microbial agent, and after seeds of a plant germinate and two pairs of true leaves grow out, roots of the plant are irrigated with the microbial agent. The prepared gongronella sp. strain can effectively promote the growth of seedlings of the plant, improve the disease resistance of crops and have application potential in the agricultural production process.

Description

technical field [0001] The invention belongs to the technical field of microbial application, and relates to a strain of Thymus globosa and its application, in particular to a fermentation preparation method of Thymella globus w5 inoculum and its application in promoting plant growth. Background technique [0002] At present, my country's per capita agricultural land is less than 1.3 mu, which is only 1 / 6 of the per capita cultivated land in the United States. With the accelerated pace of industrialization and urbanization, the amount of cultivated land in my country is still decreasing. In addition, due to factors such as low soil fertility, farmers over-apply chemical fertilizers to ensure high yields. For example, my country's current grain output accounts for 16% of the world's total, and its chemical fertilizer consumption accounts for 31% of the world's total. The amount per hectare is more than four times the world's average level. Long-term unreasonable and excessi...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/645
CPCA01N63/30C12N1/14C12N1/145C12R2001/645
Inventor 肖乐天方泽民王小洁房伟
Owner ANHUI UNIVERSITY
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