Novel application of dictamnine

A technology of leuconine and mast cells, applied in the field of medicine, can solve problems such as ineffective effects, high costs, and long treatment courses

Inactive Publication Date: 2018-06-15
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the clinical treatment of allergic diseases mainly adopts symptomatic treatment, such as the use of antihistamine drugs and hor

Method used

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  • Novel application of dictamnine
  • Novel application of dictamnine
  • Novel application of dictamnine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The purpose and method of the experiment: No matter what kind of drug, if the concentration is too high, it will produce cytotoxicity to the cells. If the concentration of whitewash is too high, it will have certain toxicity to P815 cells. In order to detect the toxicity of whitewash to P815 cells, CCK-8 experiment was used to detect the cell survival rate of P815 cells stimulated with different concentrations of whitewash for 24 hours, so as to determine the use concentration of whitewash that has no growth inhibitory activity on the cells. The specific steps are as follows:

[0019] (1) Culture and passage of mast cell line P815 cells

[0020] A. Cultivation of P815 cells: P815 was cultured in high-glucose DMEM medium containing 10% fetal bovine serum and 1% penicillin and streptomycin at 37°C and 5% CO 2 Environment;

[0021] B. Subculture of P815 cells: When the cell density in step A reaches 70%, it needs to be subcultured. Collect the cell culture solution in the...

Embodiment 2

[0029] The purpose and method of the experiment: In order to verify whether whitewashine can inhibit mast cell degranulation, mast cells were pretreated with different concentrations of whitewash, and whether it could inhibit the release of mast cell degranulation marker β-hexosaminidase was tested. Specific steps are as follows:

[0030] (1) Culture and passage of mast cell line P815 cells

[0031] A. Culture of P815 cells: P815 cultured in high-glucose DMEM medium containing 10% fetal bovine serum (FBS), 1% penicillin and streptomycin, cultured at 37°C, 5% CO 2 Environment;

[0032] B. Subculture of P815 cells: When the cell density in step A reaches 80%, it needs to be subcultured. Collect the cell culture solution in the culture bottle in a centrifuge tube, rinse with preheated phosphate buffered saline solution three times, add an appropriate amount of trypsin, After the cells are completely digested, add the cell culture medium to neutralize the trypsin, pipette evenly...

Embodiment 3

[0040] The purpose and method of the experiment: To detect the effect of leuconine on the expression of IL-4, IL-6, IL-12 and IL-13 at the mRNA level after P815 activation, the specific steps are as follows:

[0041] (1) Culture and passage of mast cell line P815 cells

[0042] A. Cultivation of P815 cells: P815 was cultured in high-glucose DMEM medium containing 10% fetal bovine serum and 1% penicillin and streptomycin at 37°C and 5% CO 2 Environment;

[0043] B. Subculture of P815 cells: When the cell density in step A reaches 70%, it needs to be subcultured. Collect the cell culture solution in the culture bottle in a centrifuge tube, rinse with preheated phosphate buffered saline solution three times, add an appropriate amount of trypsin, After the cells are completely digested, add the cell culture medium to neutralize the trypsin, pipette evenly, centrifuge at 800rpm for 3 minutes, remove the supernatant, add fresh high-sugar DMEM medium, pipette and mix the cells, and ...

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PUM

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Abstract

The invention discloses novel application of dictamnine, in particular to application of the dictamnine in preparing type I hypersensitivity reaction drugs caused by mast cell activation, and belongsto the field of pharmaceuticals. According to the dictamnine disclosed by the invention, by constructing a C48/80 induced P815 mast cell in vitro inflammatory model, the dictamnine is found to have the effects of inhibiting the activation of mast cells, effectively inhibiting the degranulation degree of the mast cells in a sensitized state and alleviating the release of beta-hexosaminidase; the expression of inflammatory genes IL-4, IL-6, IL-12 and IL-13 in the mRNA level can be inhibited; in addition, the phosphorylation level of ERK in an intracellular signaling pathway can be significantlyinhibited.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the new application of cathratron, more specifically the application of cathraine in the preparation of type I hypersensitivity drugs caused by mast cell activation. Background technique [0002] Allergic diseases (allergic diseases) include allergic rhinitis, asthma, conjunctivitis, eczema, food allergy, etc., which are common clinical diseases and frequently-occurring diseases, and have been listed by the World Health Organization (WHO) as the focus of the 21st world One of the three major diseases to be prevented and controlled is a major health problem in the world. [0003] According to the statistics of the World Allergy Organization (WAO), the incidence of allergic diseases has increased by at least 4-5 times in the past 30 years, and is increasing at a rate of more than 1% per year. The global total prevalence rate has reached 22% at present, and it is estimated that ...

Claims

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Application Information

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IPC IPC(8): A61K31/438A61P37/08
CPCA61K31/438
Inventor 安输虞姣姣徐天瑞张家静
Owner KUNMING UNIV OF SCI & TECH
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