Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting opisthopapus taihangensis total flavonoid from opisthopapus taihangensis

A technology for extracting total flavonoids of chrysanthemum and chrysanthemum, which is applied in the field of extracting total flavonoids of chrysanthemum chrysanthemum, can solve the problems of no energy consumption, good separation effect, clean process, etc., and achieve the effects of convenient operation, reduced environmental pollution, and short production cycle

Inactive Publication Date: 2018-06-15
温州瑞思生物科技有限公司
View PDF2 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In short, there have been many reports on the extraction and purification of flavonoids, but a method with low energy consumption, short time, clean process, simple process and good separation effect has not been found so far. Therefore, a new and efficient flavonoid extraction and purification technology has been developed. very necessary

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1) Preparation of Taihang Chrysanthemum total flavonoids extract: crush Taihang Chrysanthemum, pass through a 60-mesh sieve, add water and stir for 60 minutes, and ultrasonically disperse for 20 minutes to obtain an aqueous dispersion with a solid content of 10%; under the condition of a pressure of 100MPa, the The water dispersion was subjected to high-pressure homogenization treatment for 30 minutes to obtain a homogenate; the homogenate was adjusted to a pH value of 8.5-9.5 with 5wt% NaOH, stirred for 20 minutes, and alkaline cellulase, alkali Pectinase, hydrolyzed at 25°C for 2.5 hours, heated to 100°C to inactivate the enzyme for 20 minutes, then treated with a microwave with an output power of 600 W for 3 minutes, extracted with ethanol at a temperature of 70-90°C 4 times, combined extracts;

[0020] 2) Magnetic molecularly imprinted polymer: using suspension polymerization method, using rutin, quercetin, luteolin or apigenin as template molecules, oleic acid modi...

Embodiment 2

[0023] 1) Preparation of Taihang Chrysanthemum total flavonoids extract: crush Taihang Chrysanthemum, pass through a 60-mesh sieve, add water and stir for 60 minutes, and ultrasonically disperse for 10 minutes to obtain an aqueous dispersion with a solid content of 5%; under the condition of a pressure of 60MPa, the The aqueous dispersion was subjected to high-pressure homogenization treatment for 60 minutes to obtain a homogenate; the homogenate was adjusted to a pH value of 8.5-9.5 with 5wt% NaOH, stirred for 10-20 minutes, and then mixed with chrysanthemum and alkaline cellulase, The mass ratio of alkaline pectinase is: 100:0.3:0.3 Add alkaline cellulase and alkaline pectinase in turn, enzymolyze at 40°C for 2.5 hours, heat up to 100°C to inactivate the enzyme for 20min, then Treat for 3 minutes under microwave conditions with an output power of 600 W, extract 5 times with ethanol at a temperature of 90 ° C, and combine the extracts;

[0024] 2) Magnetic molecularly imprint...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting opisthopapus taihangensis total flavonoid from opisthopapus taihangensis. The method comprises the following steps: extracting flavonoid from the opisthopapus taihangensis by adopting a high-pressure homogenization method to obtain a crude extracting solution; then adsorbing the flavonoid from the crude extracting solution by utilizing a prepared magnetic molecularly imprinted polymer, so as to simply and efficiently realize separation of the flavonoid. When the method is used for extracting the opisthopapus taihangensis flavonoid from the opisthopapus taihangensis, the method has the advantages of environment friendliness, high extraction rate, good product quality, short production period, convenience for operation and relatively low cost and is suitable for industrial production.

Description

technical field [0001] The invention relates to an extraction process, in particular to a method for extracting total flavonoids of chrysanthemum chrysanthemum from chrysanthemum chrysanthemum. Background technique [0002] Flavonoids have many effects. It is a strong antioxidant that can effectively remove oxygen free radicals in the body. For example, anthocyanins can inhibit the full-stage overflow of oily peroxides. This ability to prevent oxidation is a vitamin More than 10 times that of E, this antioxidant effect can prevent cell degeneration and aging, and can also prevent the occurrence of cancer. Flavonoids can improve blood circulation and lower cholesterol. The flavonoids in Tianguo also contain a PAF anticoagulant factor. These effects greatly reduce the incidence of cardiovascular and cerebrovascular diseases, and can also improve the symptoms of cardiovascular and cerebrovascular diseases. The sky fruit from the South Pacific island country is rich in 33 flavo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28B01D11/02A61K133/00
CPCA61K36/28A61K2236/13A61K2236/19A61K2236/333B01D11/0211
Inventor 肖秀丽
Owner 温州瑞思生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com