PSP (pancreatic stone protein) detection kit with insect cell expression PSP as calibration product

Abstract

The invention relates to a PSP (pancreatic stone protein) detection kit with insect cell expression PSP as a calibration product, belongs to the biotechnological field and discloses the process as follows: the purified insect cell expression PSP is used as the calibration product, an LFIA (lateral flow immunoassay) test strip is established, the fluorescence intensity of the test strip is determined with TRFIA (time-resolved fluoroimmunoassay), and the concentration of PSP in blood or liquids is detected. The method comprises the steps as follows: extracting RNA in gastric cancer cells (AGS),synthesizing cDNA, transferring recombinant Flag-PSP AcNPV plasmids into insect cells, purifying PSP as the calibration product, marking carboxyl modified particles, assembling the LFIA test strip andestablishing an automatic PSP concentration display system. The kit has the advantages of being high in sensitivity, high in specificity, convenient to operate and capable of rapidly diagnosing bacterial infection and sepsis.

Description

technical field [0001] The invention belongs to the field of biotechnology. Background technique [0002] Bacterial infection, sepsis, septic shock, and multiple organ failures often occur in critically ill patients, leading to death. It is necessary to quickly and accurately determine the occurrence and development of bacterial infection and sepsis, and carry out timely and effective treatment. Clinically, procalcitonin (PCT) and C-reactive protein (C-reactive protein, CRP) indicators in serum are commonly used to determine the occurrence and severity of bacterial infection and sepsis. [0003] The level of CRP in serum can reflect the severity of body inflammation. CRP is an emergency response protein. Viral infection, bacterial infection, and body inflammation can all cause the increase of CRP. It is difficult to distinguish between infectious diseases and Other inflammatory diseases, less specific. [0004] As a commonly used inflammatory indicator, PCT has strong spec...

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Problems solved by technology

[0009] Some researchers dynamically monitored the PSP, CRP, and WBC indicators of patients after cardiac surgery for 3 consecutive days, and all the three indicators increased after surgery; the PSP level increased on the 1st, 2nd, and 3rd days after surgery, which can be used to judge postoperative bacterial infection However, the increase of CRP and WBC indexes has no value in judging postoperative bacterial infection

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