A method for extracting heat-denatured rice bran protein
A technology for rice bran protein and rice bran, which is applied in the field of extracting heat-denatured rice bran protein, can solve the problems of low extraction rate of heat-denatured rice bran protein and poor color of rice bran protein products, achieves good color and luster, high protein extraction rate, and solves the problem of poor color and luster. Effect
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Embodiment 1
[0035] This embodiment provides a method for removing starch and removing phytic acid to treat high-temperature defatted rice bran protein. The specific method is as follows:
[0036] 1) De-starch method: crush high-temperature degreased rice bran, pass through a 50-mesh sieve, wash 3 times with water to remove impurities such as ash, mix with distilled water, then adjust the mash concentration to 25% (mass), adjust the pH value to 6, and heat to 87°C , add defatted rice bran 0.1% (mass) of Termamyl SC (temperature-resistant amylase) to react for 60 minutes and filter to obtain a filter residue, which is rice bran residue after starch removal;
[0037] 2) Method for removing phytic acid: pulp the starch-removed rice bran residue obtained in step 1) until the mash concentration is 10% (mass), adjust the pH value to 2.5 with 5% hydrochloric acid, react at 40°C for 60 minutes, and filter to obtain the filter residue , the obtained filter residue is the rice bran residue of removi...
Embodiment 2
[0042] This embodiment provides a method for extracting heat-denatured rice bran protein, the method comprising the following steps:
[0043] Step 1: The high-temperature defatted rice bran is subjected to starch-removing and phytic-acid-removing treatment, and then dried to a constant weight to obtain a starch-removing and phytic-acid-removing rice bran residue. The rice bran protein content in the above residue was measured, and the measured protein content was 28%.
[0044] Step 2: Weigh 1.67 parts by weight of starch-removed phytic acid residues and disperse them into a mixture of 50 parts by weight of distilled water and ethanol, wherein the volume ratio of distilled water and ethanol in the mixture is 4:1, and add the above-mentioned mixed feed liquid Go to the subcritical device and keep at 180°C for 30 minutes; after the reaction, put the reaction device into an ice-water bath to cool it down rapidly, and filter the slurry therein to obtain the filtrate. Take the filt...
Embodiment 3
[0067] In this example, the effect of the solid-liquid ratio of the mixture of starch-removed phytic acid residue, distilled water and ethanol on the extraction of heat-denatured rice bran protein was investigated. The amount is mixed, the specific steps are as follows:
[0068] Step 1: The high-temperature defatted rice bran is subjected to starch-removing and phytic-acid-removing treatment, and then dried to a constant weight to obtain a starch-removing and phytic-acid-removing rice bran residue. The rice bran protein content in the above residue was measured, and the measured protein content was 28%.
[0069] Step 2: Weigh the starch-removed phytic acid residue and disperse it in the mixed solution of distilled water and ethanol, wherein the volume ratio of distilled water and ethanol in the mixed solution is 4:1. Keep at ℃ for 30 minutes; After the reaction is completed, put the reaction device into an ice-water bath to cool it rapidly, and suction-filter the slurry there...
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