Fluorescent silver nanometer cluster with stable nucleic acid, and preparation method and application thereof to toxin detection

A silver nanocluster, fluorescence technology, applied in nanotechnology, nanotechnology, nano-optics and other directions, can solve the problems of long time and high cost, and achieve the effect of simple operation, short time consumption and high sensitivity

Inactive Publication Date: 2018-06-29
WENZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods are more popular, they all require a stable source of antibodies, and they are time-consuming and expensive

Method used

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  • Fluorescent silver nanometer cluster with stable nucleic acid, and preparation method and application thereof to toxin detection
  • Fluorescent silver nanometer cluster with stable nucleic acid, and preparation method and application thereof to toxin detection
  • Fluorescent silver nanometer cluster with stable nucleic acid, and preparation method and application thereof to toxin detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Preparation of AgNCs emitting infrared light and its application in biosensors

[0046] (1) Synthesis of DNA-stabilized AgNCs emitting infrared light

[0047] Preparation of fluorescent silver nanoparticles: the single chain concentration in the system is 1.5 μM. In the total reaction system, add 10mMPBS buffer solution, the signal probe P5 required for the synthesized infrared-emitting AgNCs prepared in advance, and an appropriate concentration of silver nitrate solution, mix for 30s and rest for 15 minutes, then add an appropriate concentration of The sodium borohydride solution was mixed evenly, and after reacting for 12 hours under the condition of avoiding light, it was placed in the refrigerator for later use.

[0048] (2) Using DNA-stabilized infrared-emitting AgNCs as fluorescent probes to analyze OTA toxins in food

[0049] In the total reaction system, add 20 μL of the above solution of AgNCs that emits infrared light, 100 μL of buffer solution, a...

Embodiment 2

[0051] Example 2: Preparation of AgNCs emitting near-infrared light and its application in biosensors

[0052] (1) Synthesis of DNA-stabilized AgNCs emitting near-infrared light

[0053] Preparation of fluorescent silver nanoparticles: the single chain concentration in the system is 1.5 μM. In the total reaction system, add 10mMPBS buffer solution, the prepared signal probe P5 required by the synthesized AgNCs that emit near-infrared light, and an appropriate concentration of silver nitrate solution. The sodium borohydride solution was mixed evenly, and after reacting for 12 hours under the condition of avoiding light, it was placed in the refrigerator for later use.

[0054] (2) DNA-stabilized near-infrared-emitting AgNCs were used as fluorescent probes to analyze AFB1 toxin in food

[0055] In the total reaction system, add 20 μL of the above solution of AgNCs emitting near-infrared light, 100 μL of buffer solution, and an appropriate amount of WS 2 , an appropriate amoun...

Embodiment 3

[0057] Example 3: AgNCs that emit infrared light and AgNCs that emit near-infrared light are applied to biosensors at the same time

[0058] (1) Synthesis of DNA-stabilized AgNCs

[0059] Preparation of silver nanoparticles emitting near-infrared light: the single-chain concentration in the system is 1.5 μM. In the total reaction system, add 10mMPBS buffer solution, the signal probe P6 required by the synthesized near-infrared-emitting AgNCs prepared in advance, and an appropriate concentration of silver nitrate solution, mix for 30s and rest for 15 minutes, then add an appropriate concentration The sodium borohydride solution was mixed evenly, and after reacting for 12 hours under the condition of avoiding light, it was placed in the refrigerator for later use.

[0060] Preparation of silver nanoparticles emitting infrared light: the single chain concentration in the system is 1.5 μM. In the total reaction system, add 10mM PBS buffer solution, the signal probe P5 required f...

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Abstract

The invention provides a fluorescent silver nanometer cluster with stable nucleic acid, and a preparation method and application thereof to toxin detection. The method comprises the following steps ofdesigning two kinds of silver nanometer clusters (AgNCs) with stable nucleic acid, wherein a sequence connected onto the AgNCs with stable DNA can be a toxin aptamer sequence; when the sequence connected onto the AgNCs with stable DNA is an ochratoxin (OTA) aptamer, under the OTA existence condition, the action force of the OTA and the aptamer at one end of the aptamer with stable DNA is greaterthan the action force between the single-chain DNA and the WS2, so that the AgNCs is disengaged from WS2; the fluorescence is further recovered. In addition, when the aptamer containing two kinds of different toxins in the solution is connected with DNA-stable AgNCs of different light, two kinds of detection targets are added at the same time; the simultaneous detection of the two kinds of toxin can be realized. The method has the advantages of high selectivity, high sensitivity and low cost; the OTA and AFB1 detection lower limit is respectively 0.5ng/mL and 0.5 ng/mL.

Description

technical field [0001] The invention belongs to the research field of nanocomposite materials, and particularly relates to a method for preparing a stable fluorescent silver nanocluster composite material using a specific nucleic acid sequence as a template. This material combines tungsten disulfide similar to graphene nano The properties of the three-dimensional layered structure are used in a novel fluorescent biosensor for the simultaneous detection of multiple toxins, and the nucleic acid-stabilized silver nanoclusters can be recorded as AgNCs. Background technique [0002] Mycotoxins in animal feed and human food are very harmful to the health of animals and humans, which has aroused great attention from people all over the world to the detection of mycotoxins. Ochratoxin A and aflatoxin B1 are two of the most prevalent mycotoxins. These two toxins are the most common and most toxic in natural foods (such as cereals, beans, dried fruits, coffee, etc.). In the prior ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/58G01N21/64B22F9/24B82Y20/00B82Y40/00
CPCB22F9/24B82Y20/00B82Y40/00C09K11/58G01N21/6428
Inventor 聂华贵王宇来倩倩杨植顾灿灿黄少铭
Owner WENZHOU UNIVERSITY
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