Method for preparing nano-micro-spherical type human papilloma virus resistant and gynecologic inflammation pathogene polyclone combined IgY antibody

Abstract

The invention discloses a method for preparing a nano-micro-spherical type human papilloma virus resistant and gynecologic inflammation pathogene polyclone combined IgY antibody. The method comprisesthe following steps: A, screening combined pathogene with a high-risk HPV and gynecologic inflammation pathogene; B, introducing the combined pathogene into engineering bacteria, and expressing combined antiogen by using the engineering bacteria; C, injecting the combined antiogen into livestock so as to obtain an egg with a polyclone yolk immune globulin combined IgY antibody; D, separating and purifying a combined IgY antibody extract of the combined IgY antibody for preventing the HPV and the gynecologic inflammation pathogene from the egg; e, concentrating the combined IgY antibody extract, and performing freezing drying, thereby obtaining the nano-micro-spherical type human papilloma virus resistant and gynecologic inflammation pathogene polyclone combined IgY antibody. The polyclonecombined IgY antibody prepared according to the method has the characteristics of high activity, good stability, good specificity, high safety and good permeability, and can be applied to treatment and auxiliary treatment on HPV infection and mixed infection of multiple gynecologic viruses and pathogenic bacteria.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to a preparation method of a polyclonal combined IgY antibody against human papilloma virus and gynecological inflammatory pathogens. Background technique [0002] Cervical cancer (CC) is a tumor with a high incidence in women, and its incidence rate is second only to breast cancer. In 1992, WHO announced that HPV virus (human papillomavirus, HPV) infection is the primary factor for cervical canceration. In 1995, the International Cancer Society believed that HPV is the main cause of CC. Epidemiological studies have shown that HPV infection can cause cervical precancerous lesions, namely cervical intraepithelial neoplasia (CIN), which progresses slowly to CC. A large-scale study involving 22 countries showed that HPV virus, human papillomavirus, was detected in 99.7% of CC patient tissue samples. Current studies show that in natural populations, HPV infection...

AI Technical Summary

Problems solved by technology

However, when people take antibiotics or clean the vagina with ordinary lotions, antiseptic effervescent tablets or suppositories, the beneficial bacteria such as Lactobacillus in the vagina will also be killed, thereby destroying the flora ecology that acts as a natural barrier in the vagina balance, causing various pathogens to take advantage of the opportunity to multiply in large numbers; as a result, many women suffer from various gynecological infections and HPV virus infection for life because there is no cure

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