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Portable enhanced surface plasmon resonance biosensor

A sensor and ion technology, applied in the field of ion resonance biosensors, can solve the problems that are difficult to adapt to simple, fast, accurate, sensitive, on-site tracking detection, sensors rely on large instruments and equipment, and time-consuming, etc., to achieve GH displacement measurement , Accurate and reliable GH displacement measurement, the effect of improving stability and reliability

Pending Publication Date: 2018-07-10
PEKING UNIV SHENZHEN GRADUATE SCHOOL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing sensors still have problems such as relying on large-scale instruments and equipment, time-consuming or requiring specialized technicians to operate, and it is difficult to adapt to the needs of simple, fast, accurate, sensitive, on-site tracking detection, and the cost is high

Method used

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  • Portable enhanced surface plasmon resonance biosensor
  • Portable enhanced surface plasmon resonance biosensor
  • Portable enhanced surface plasmon resonance biosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The preparation of embodiment 1 sensor

[0067] Such as figure 1 As shown, all optical components used in the sensor are placed and sealed in a light-tight waterproof module, and are connected to the external laser light source and circuit board through an adapter and connection port. The size of the single beam emitted by the two laser diodes is limited to about 200 microns by a microhole, respectively, and passes through the p- and s-polarizers respectively, and is modulated by the inverse optical chopper, that is, when the p-polarized beam passes through, s - The polarized beam is blocked, and vice versa, the two beams alternate at a frequency of about 240Hz, pass through an optical mirror combination consisting of a mirror, a polarizing beam splitter and a convex lens, the two beams merge into the same optical path, and then focus The lens is refracted onto the entrance face of the gold-coated prism that makes up the sensor chip. The distances from the lens to the...

Embodiment 2

[0070] Embodiment 2 single reflection GH-SPR test

[0071] Using the sensor of Example 1, a single reflection GH-SPR test was performed using optimized SPR conditions (gold film thickness 50nm, laser wavelength 650nm, incident angle 45 degrees), and a significant GH shift was measured. Such as Figure 4 As shown, after coupling SPR, the GH displacement of the sensor of the present invention is significantly increased, and the noise is significantly reduced by using a 16-bit analog-to-digital converter. Moreover, after the system is equipped with a black casing, environmental interference is shielded, and the signal quality is significantly improved.

Embodiment 3

[0072] Example 3 Detection of Hg Using Double-Stranded DNA Containing T-T Mismatches 2+ detection method

[0073] Such as Figure 5 As shown in (A), a single-stranded DNA is immobilized on the surface of the GH-SPR sensor gold membrane through a sulfhydryl group, and the other single-stranded DNA that is mismatched with T-T cannot form a stable hybrid double-stranded DNA, but has Hg 2+ When present, with Hg 2+ Multiple T-Hg-T coordination structures are formed to obtain stable hybrid double-stranded DNA, which forms a different SPR response from a single-stranded DNA on the sensor surface, resulting in changes in GH displacement. The length of the DNA chain, the number and position of T-T mismatches are the key to the design of this type of DNA, and the optimization of these parameters will improve the detection effect. Captured DNA and Hg 2+ It can be eluted with cysteine ​​solution, so that the ONP immobilized on the surface of the sensor can be reused. After testing, t...

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Abstract

The invention relates to a portable enhanced surface plasmon resonance biosensor and belongs to the field of heavy metals detection. The portable enhanced surface resonance biosensor as a GH-SPR (Goos-Hanchen surface plasmon resonance) sensor, comprising a waterproof module, wherein the waterproof module is connected with an external laser source through an adapter and is connected with an external control circuit through a connection port. ONP (open network provisioning) technology is combined with the GH-SPR sensor, and SPR coupling effect more significant than heavy metal ions themselves iscreated through connection or disconnection of DNA strands and additional AuNPs to the surface of the sensor. The sensor is simple in structure and easy to use; a DNA probe method has the advantagesof low sampling quantity, zero need for marking, isolation and purification, short detection time and the like; a DNA chip is reusable so that the cost is further reduced.

Description

technical field [0001] The invention relates to a portable enhanced surface plasmon resonance biosensor, which belongs to the field of analysis and detection instruments. Background technique [0002] Heavy metal pollution has become one of the focuses of public attention in recent years because of its seriousness, persistence and extensiveness. Therefore, heavy metal detection is very important in medicine, food and the environment. The portable heavy metal detector based on GH-SPR-DNA technology is mainly used for emergency and on-site detection. The target market is mainly water companies that detect tap water quality, environmental protection departments that monitor heavy metal pollution, and regulatory departments for agricultural environment, agricultural products and food heavy metal pollution. Other areas involved include industrial enterprises involved in heavy metal wastewater (such as electroplating, surface treatment, etc.). [0003] Surface Plasmon Resonance (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/552G01N21/01
CPCG01N21/01G01N21/553G01N2021/0112G01N2201/068G01N2201/0668G01N2201/0221
Inventor 吴华南
Owner PEKING UNIV SHENZHEN GRADUATE SCHOOL
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