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Preparation of gene sequencing calibration reference strain

A gene sequencing and gene mutation technology, applied in the medical field, can solve the problem of no reference strains for gene sequencing calibration

Pending Publication Date: 2018-07-20
翁炳焕
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, there is no such gene sequencing calibration reference strain

Method used

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  • Preparation of gene sequencing calibration reference strain
  • Preparation of gene sequencing calibration reference strain
  • Preparation of gene sequencing calibration reference strain

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Experimental program
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Embodiment Construction

[0013] figure 1 It is a schematic diagram of IgG fusion aiding of the present invention.

[0014] figure 2 It is a schematic diagram of SPA synergistic double antibody fusion of the present invention.

[0015] image 3 It is the real picture of cell fusion of the present invention

[0016] Such as figure 1 , 1 represents a myeloma cell with IgG Fab receptor, 2 represents a monoclonal antibody (IgG), 3 represents a genetically mutated cell with an IgG Fc receptor, and the other Fab end of a monoclonal antibody (IgG) can also bind a myeloma cell , The cells to be fused are connected together by monoclonal antibodies, which is conducive to fusion under the action of PEG.

[0017] Such as figure 2 , 1 represents the Staphylococcus aureus protein A (SPA) with IgGFc receptor, 2 represents the monoclonal antibody of the cells to be fused with numbers 6 and 7, 3 represents the monoclonal antibodies of the cells to be fused with numbers 4 and 5, After the monoclonal antibody [...

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Abstract

The invention discloses the preparation of a gene sequencing calibration reference strain and belongs to the field of medicines. The preparation is characterized in that a gene mutant cell with an IgGFc acceptor is connected with a myeloma cell with an IgGFab acceptor to form a complex; or after being respectively connected with IgG, the gene mutant cell and the myeloma cell which both have the IgGFc acceptor are connected with SPA with the IgGFab acceptor, furthermore under the action of polyethylene glycol, or under the direct function of the polyethylene glycol, the gene mutant cell and themyeloma cell are combined into a hybridoma cell, a mutant gene transplantation tumor cell can be unlimitedly amplified along with tumor cells, through identification of a target pathogenic gene, thegene sequencing calibration reference strain can be prepared, the strain can be applied to whole-process quality control, quality evaluation and calibration upon gene sequencing, or a conventional gene comparison database is substituted to compare sequencing data with mutant genes, and a conventional gene sequencing method can be improved.

Description

technical field [0001] The invention relates to the preparation of a gene sequencing calibration reference strain in the medical field, and is mainly used for error correction of DNA gene mutation sequence detection. Background technique [0002] Gene Sequencing, or DNA Sequencing, refers to the analysis of the base sequence of a specific DNA fragment. The emergence of gene sequencing technology not only promotes the research of genomics, but also provides new ideas for the etiology research of complex diseases, and also promotes the application of genetic testing in prenatal diagnosis, organ transplant matching, tumor molecular diagnosis and targeted therapy. And the application of individualized drug therapy. Gene sequencing technology has been developed to the fourth generation, and the second, third and fourth generation sequencing technologies are collectively referred to as next generation sequencing (NGS) technology. The principle of gene sequencing has correspondin...

Claims

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Application Information

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IPC IPC(8): C12N5/10C12N15/06
CPCC07K16/2896C12N5/163C12N2510/00
Inventor 翁炳焕李兰娟
Owner 翁炳焕
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