Preparation method of jujube flower paraffin slices

A technology for jujube flower paraffin and jujube flower, which is applied in the field of preparation of jujube flower paraffin sections, can solve the problems of unsatisfactory sectioning effect, incomplete section fragmentation, unclear tissue structure, etc., and achieves increased staining effect and clear and complete tissue structure. , the effect of shortening the dyeing time

Inactive Publication Date: 2018-07-27
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few studies on paraffin section technology using jujube flowers as materials, and the sectioning effect is not particularly ideal. The main problem is that jujube flowers are small and tender, which may easily cause incomplete slices and unclear tissue structures, which is not conducive to observation.

Method used

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  • Preparation method of jujube flower paraffin slices
  • Preparation method of jujube flower paraffin slices
  • Preparation method of jujube flower paraffin slices

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preparation example Construction

[0053] The invention provides a kind of preparation method of jujube flower paraffin section, comprises the steps:

[0054] 1) Put jujube flower buds in the fixative solution and fix them for 24-36 hours to obtain fixed jujube flowers;

[0055] The jujube flower bud has a stem section with a length of 0.3 to 0.5 cm;

[0056] The fixative solution is obtained by configuring formaldehyde solution with a volume concentration of 38%, glacial acetic acid and ethanol solution with a volume concentration of 50% in a volume ratio of 0.95 to 1.05:0.95 to 1.05:17.5 to 18.5;

[0057] 2) dehydrating the fixed jujube flower in the step 1) to obtain dehydrated jujube flower;

[0058] 3) mixing and soaking the dehydrated jujube flower in the step 2) and the first clearing agent to carry out the first transparent treatment to obtain the first transparent jujube flower;

[0059] The first transparent agent is a mixed solution formed by mixing absolute ethanol and xylene in a volume ratio of ...

Embodiment 1

[0121] Cut two jujube flower buds with different flowering times from the branches of jujube tree, which are 1.21mm in diameter and green-yellow jujube flower bud and 2.00mm in diameter and yellow-green jujube flower bud in color, and mark them in turn For No. 1 and No. 2, these two jujube flower buds are respectively followed by the following steps:

[0122]Retain the stem section of 0.3cm in the jujube flower bud base, place the fixative (fixative solution is: formaldehyde aqueous solution, glacial acetic acid and the ethanol aqueous solution that the volume concentration is 50% by volume ratio are 0.95:1.05: 17.5 configuration) fixed for 36h, get fixed Zaohua. Rinse the obtained jujube flowers with 30% ethanol aqueous solution for 3 minutes, and then soak them with 50%, 60%, 70%, 80%, 90% ethanol aqueous solution and absolute ethanol for 0.9h respectively , replaced with absolute ethanol and then soaked for 35 minutes to obtain dehydrated jujube flowers. The obtained dehy...

Embodiment 2

[0126] Cut two jujube flower buds with different flowering times from the branches of jujube tree, namely the jujube flower bud with a diameter of 1.50mm and a greenish yellow color and the jujube flower bud with a diameter of 2.23mm and a yellow-green color, and mark them in turn For No. 3 and No. 4, these two jujube flower buds are respectively followed by the following steps:

[0127] Retain the stem segment of 0.5cm at the jujube flower bud base, place the fixative (the fixative is: formaldehyde aqueous solution, glacial acetic acid and the ethanol aqueous solution that the volume concentration is 50% by volume ratio are 1.05:0.95: 18.5 configuration) was fixed for 24 hours, and fixed Zaohua was obtained. The obtained fixed jujube flowers were first rinsed with 30% ethanol aqueous solution for 1 min, and then soaked with 50%, 60%, 70%, 80%, 90% ethanol aqueous solution and absolute ethanol for 1.1 h respectively. , replaced with absolute ethanol and then soaked for 25 min...

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Abstract

The invention provides a preparation method of jujube flower paraffin slices, and belongs to the technical field of paraffin slices. The preparation method comprises the following steps that (1) jujube flower buds are fixed; the jujube flower buds have one stem segment with the length being 0.3 to 0.5cm; (2) dehydration; (3) first transparency treatment; (4) second transparency treatment; (5) waxing; (6) embedding; (7) wax trimming; (8) slicing; (9) slice unfolding; (10) dewaxing; (11) rehydration; (12) secondary rehydration; (13) dyeing; (14) slice covering and slice sealing. The preparationmethod of the jujube flower paraffin slices provided by the invention has the advantages that the complete longitudinal section of the megaspore can be obtained; further, the jujube flower cellular morphology and the development change of the megaspore inside cell and tissue structures can be conveniently observed; meanwhile, the basic rule in the megaspore development structure change and reproduction process can be obtained through observing the jujube flower paraffin slices.

Description

technical field [0001] The invention belongs to the technical field of paraffin wax sections, and in particular relates to a preparation method of jujube flower paraffin wax sections. Background technique [0002] Jujube (Ziziphus jujuba Mill.) is native to China and is an important economic tree species. Its fruit is beautiful in shape and delicious in taste, rich in various amino acids, vitamins, polysaccharides and other beneficial substances needed by the human body. It has high therapeutic value and health care effect, and is deeply loved by consumers. Hybrid breeding is currently one of the most important methods for cultivating new varieties of fruit trees. However, jujube hybrid breeding has lingered for a long time. The main reason is that the flowers of jujube trees are hermaphroditic flowers, and the flowers are very small, which makes it difficult to detassele, and the flower and fruit drop is serious. Biological characteristics such as low yield rate are not co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 李颖月黄飞逸崔艳红庞晓明李翔
Owner BEIJING FORESTRY UNIVERSITY
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