Super-resolution microimaging system and imaging method based on femtosecond pulse shaping

A technology of femtosecond pulse shaping and pulse shaping, which is applied in the field of microscopy, can solve the problems of universal limitation, no realization of shaped pulse modulation, no multi-dimensionality, etc., and achieve high efficiency of optical path, wide-field observation of optical path, and simple optical path Effect

Active Publication Date: 2018-07-27
PEKING UNIV
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Problems solved by technology

However, there is currently no method for multi-particle and multi-dimensional active regulation of particles.
For example, the STED and STORM methods belong to active regulation, but are based on single-particle luminescence, and do not realize multi-particle excitation control; the SOFI method belongs to multi-particle, but belongs to

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  • Super-resolution microimaging system and imaging method based on femtosecond pulse shaping
  • Super-resolution microimaging system and imaging method based on femtosecond pulse shaping
  • Super-resolution microimaging system and imaging method based on femtosecond pulse shaping

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Example Embodiment

[0039] Example one

[0040] Such as figure 1 As shown, the super-resolution microscopy imaging system based on femtosecond pulse shaping of this embodiment includes: femtosecond pulse excitation light source 1, pulse shaping system 2, spectroscope 4, objective lens 5, sample adjustment table 6, signal collection system 10 And computer control system 11; among them, femtosecond pulse excitation light source 1 generates femtosecond pulse laser, which is broadband light, including light of different frequency components; pulse shaping system 2 phases light of different frequency components Modulation, changing the femtosecond pulse laser into a set of shaped pulses whose intensity, polarization and frequency change with time as excitation light; after the excitation light passes through the beam splitter 4, it is focused by the objective lens 5 and irradiated on the sample on the surface of the sample adjustment table 6 Excite the sample to produce fluorescence, scattered light, and...

Example Embodiment

[0041] Example two

[0042] Such as figure 2 As shown, the super-resolution microscopy imaging system based on femtosecond pulse shaping of this embodiment includes: femtosecond pulse excitation light source 1, pulse shaping system 2, excitation light beam expansion collimation system 3, beam splitter 4, objective lens 5, The sample adjustment table 6, the filter 7, the reflector 8, the signal light focusing transmission 9, the signal collection system 10 and the computer control system 11. Among them, the femtosecond pulse excitation light source 1 generates the femtosecond pulse laser, which is broadband light, including Multiple light of different frequency components; the pulse shaping system 2 phase-modulates the light of different frequency components to transform the femtosecond pulse laser into a set of shaped pulses whose intensity, polarization and frequency change with time as excitation light; The excitation light is expanded and collimated by the excitation light be...

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Abstract

The invention discloses a super-resolution microimaging system and an imaging method based on femtosecond pulse shaping. In the invention, a pulse shaping system is controlled by a computer control system so as to performing modulation in the aspects of phase position, intensity and polarization of light with different frequency components, so that femtosecond pulse laser becomes shaped pulse, ofwhich the intensity, polarization and frequency are related to time, as an exciting light; by using the exciting light for exciting a sample, particles, having different properties and types, in the sample have different response to the exciting light, so that responding intensity of a signal light relatively changes; when the pulse modulation changes, an signal light image is changed too; the computer control system, via a graphic processing algorithm, analyses and processes a plurality of the signal light images, thus acquiring a super-resolution image of the sample. The system and method overcome defects in methods in the prior art, has universality, is simple in optical path, allows wide-field observation, and is high in efficiency.

Description

technical field [0001] The invention relates to microscope technology, in particular to a super-resolution microscopic imaging system based on femtosecond pulse shaping and an imaging method thereof. Background technique [0002] Super-resolution microscopy has an important impact in the fields of living biological tissue imaging and material characterization. Among them, stimulated radiation depletion microscopy (STED), stochastic optical reconstruction microscopy (STORM), optical wave super-resolution imaging (SOFI), etc., utilize different characteristics of fluorescent molecules to obtain fluorescent molecules through modulation or acquisition. A microscopic technique for super-resolution imaging under different luminescence conditions. Compared with other imaging methods, the principle is relatively simple, it can perform super-resolution imaging of living tissue, and has the advantages of high spatial resolution. It has very huge applications in the fields of biomedic...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6458
Inventor 王树峰刘聪越李东海褚赛赛刘伟龚旗煌
Owner PEKING UNIV
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