Micro-fluidic chip detection system, and method used for sample detection based on micro-fluidic chip detection system

Abstract

The invention provides a micro-fluidic chip detection system, and a method used for sample detection based on the micro-fluidic chip detection system. The micro-fluidic chip detection system comprisesa micro-fluidic detection chip. The micro-fluidic detection chip comprises a micro fluid chamber; a micro well array which is arranged on the bottom surface in the micro fluid chamber; a sample inletguide pipe and a sample outlet guide pipe which are connected with the two sides of the micro fluid chamber respectively; a micro fluid operation device which is used for introducing samples reactedwith microspheres into the micro fluid chamber through the sample inlet guide pipe, realizing precipitation of the samples reacted with the microspheres into micro wells through controlling flow speed; an image acquisition device which is arranged above the micro well array, and is used for collecting information of the samples precipitated into the micro wells; and a data analysis device which isused for analyzing sample information collected by the image acquisition device and obtaining analysis results. The micro-fluidic chip detection system is beneficial for realizing of high flux, non-blocked, portable, low-detection-limit detection on samples.

Description

technical field [0001] The invention relates to the fields of life science and medicine, and in particular, to a microfluidic chip detection system and a sample detection method based on the system. Background technique [0002] Biomarkers are effective means to detect and evaluate routine biological processes, pathogenesis, or pharmacological responses to therapeutic interventions. Therefore, the convenient and accurate detection and quantification of biomarkers is a core goal of modern biotechnology. For clinical applications, sandwich immunoassays are the most widely used means of detecting biomarkers of interest. Generally, these methods require some type of label (enzymatic or fluorescent, etc.) to selectively and sensitively detect the target analyte. It also requires specific equipment (such as a plate reader) and many washing steps. Recently, the rapid development of rapid, accurate, portable, and marker-free methods for quantifying biomarkers has made the concept ...

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present application provides a microfluidic chip detection system and a method for sample detection based on the system to overcome the difficulty of detection due to low throughput, easy blockage, and low resolution in the prior art. question

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