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Preparation method for xylooligosaccharide

A technology for xylo-oligosaccharide and sugarcane molasses, which is applied in the field of xylo-oligosaccharide production and can solve the problems of technical difficulty, excessive waste water, large environmental pollution and the like

Inactive Publication Date: 2018-08-03
SUZHOU KUNLAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Acid hydrolysis is a method of partially hydrolyzing xylan with hydrochloric acid or sulfuric acid to obtain xylo-oligosaccharides. Due to the shortcomings of high technical requirements, many harmful substances, complicated processes, and low yields, this method is no longer used for production.
[0005] The alkaline hydrolysis method is to treat the raw material with dilute lye, remove part of the lignin in the raw material, and then use xylanase for enzymatic hydrolysis to prepare it. This method has disadvantages such as large amount of lye used, complicated process, and more waste water, which causes environmental pollution. bigger
[0006] The hot water extraction method is to use hot water (including steam explosion method) to pretreat the raw materials, and then perform enzymatic hydrolysis to obtain xylooligosaccharides. This method requires temperature-resistant and pressure-resistant equipment, consumes a lot of energy, and the product is obtained Disadvantages such as low rate
[0007] Enzymatic hydrolysis refers to the method of directly using enzymes (or immobilized enzymes) to process raw materials to produce xylooligosaccharides. This method has high production costs and technical difficulties. At present, this method is rarely used for industrial production
[0008] At present, the industrial production of xylo-oligosaccharides mainly uses two methods: alkaline hydrolysis and hot water extraction (including steam explosion method). These two methods generally have complex processes, high energy consumption, high waste water, large pollution and low product yield. However, there is an urgent need for a xylooligosaccharide production and preparation method with simple process, low consumption, low pollution and high yield.

Method used

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  • Preparation method for xylooligosaccharide
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  • Preparation method for xylooligosaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A preparation method of xylooligosaccharides, comprising the following steps:

[0023] (1) Crush bagasse into bagasse powder with a diameter of less than 0.5mm, weigh 100kg of bagasse powder, 800kg of water and 100kg of sugarcane molasses (total sugar content 46.7%, total number of colonies 37,000 CFU / g) and mix to obtain the first mixture ;

[0024] (2) Adjust the pH of the first mixture in step (1) to 4.50 with 0.2 kg of phosphoric acid, raise the temperature to 45°C, add 1 kg of compound enzyme preparation, and maintain this temperature for 2 hours, wherein the compound enzyme preparation consists of 20% β-glucanase, 25% pectinase, 35% acid cellulase and 20% acid protease are composed to obtain the second mixture;

[0025] (3) The second mixture of step (2) was sterilized by damp heat at 121°C for 20 minutes, cooled to 40°C after sterilization, 3 kg of Aspergillus niger strain was added, cultured and fermented at 40°C for 50 hours to obtain a fermentation liquid;

...

Embodiment 2

[0030] A preparation method of xylooligosaccharides, comprising the following steps:

[0031] (1) Crush bagasse into bagasse powder with a diameter of less than 0.5mm, weigh 100kg of bagasse powder, 800kg of water and 100kg of sugarcane molasses (total sugar content 45.1%, total number of colonies 33,000 CFU / g) and mix to obtain the first mixture ;

[0032] (2) Adjust the pH of the first mixture in step (1) to 4.60 with 0.2 kg of phosphoric acid, raise the temperature to 46°C, add 1 kg of compound enzyme preparation, and maintain this temperature for 4 hours, wherein the compound enzyme preparation consists of 25% Beta-glucanase, 30% pectinase, 25% acid cellulase and 20% acid protease are composed to obtain the second mixture;

[0033] (3) The second mixture of step (2) was sterilized by damp heat at 121°C for 30 minutes, cooled to 40°C after sterilization, 3 kg of Aspergillus niger strain was added, cultured and fermented at 45°C for 50 hours to obtain a fermentation liquid;...

Embodiment 3

[0038] A preparation method of xylooligosaccharides, comprising the following steps:

[0039] (1) Crush bagasse into bagasse powder with a diameter of less than 0.5mm, weigh 100kg of bagasse powder, 800kg of water and 100kg of sugarcane molasses (total sugar content 47.7%, total number of colonies 31,000 CFU / g) and mix to obtain the first mixture ;

[0040] (2) Adjust the pH of the first mixture in step (1) to 5.00 with 0.23 kg of phosphoric acid, raise the temperature to 55°C, add 1 kg of compound enzyme preparation, and maintain this temperature for 4 hours, wherein the compound enzyme preparation consists of 20% Beta-glucanase, 30% pectinase, 30% acid cellulase and 20% acid protease are composed to obtain the second mixture;

[0041] (3) The second mixture of step (2) was sterilized by damp heat at 121°C for 25 minutes, cooled to 40°C after sterilization, 3 kg of Aspergillus niger strain was added, cultured and fermented at 42°C for 50 hours to obtain a fermentation liquid...

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Abstract

The invention provides a preparation method for xylooligosaccharide. The preparation method comprises the following steps: smashing bagasse into powder, mixing the bagasse powder, water and cane molasses to obtain a first mixture; carrying out pH (Potential of Hydrogen) value regulation and temperature raising on the first mixture, adding a complex enzyme preparation, and carrying out constant temperature processing to obtain a second mixture; carrying out moist heat sterilization and cooling on the second mixture, adding an aspergillus niger strain, and carrying out stable culture and fermentation to obtain fermentation broth; carrying out plate-frame pressure filtration on the fermentation broth, and adding filtering and color removal auxiliary agent to obtain filter slags and clear solution; drying and smashing filter slags to obtain xylooligosaccharide used for feeding; carrying out vacuum spray drying on the clear solution to obtain high-purity xylooligosaccharide.

Description

technical field [0001] The invention relates to the technical field of xylooligosaccharide production, in particular to a preparation method of xylooligosaccharide. Background technique [0002] Xylooligosaccharides, also known as xylooligosaccharides, are oligosaccharides formed by connecting 2-10 xylose molecules with (β-1-4) glycosidic bonds. Xylooligosaccharides have significant effects in human health care and animal breeding . [0003] The existing preparation methods of xylo-oligosaccharides include acid hydrolysis, alkali hydrolysis, hot water extraction (including steam explosion method) and enzymatic hydrolysis. [0004] Acid hydrolysis is a method of partially hydrolyzing xylan with hydrochloric acid or sulfuric acid to obtain xylooligosaccharides. Due to the shortcomings of high technical requirements, many harmful substances, complicated processes, and low yields, this method is no longer used for production. [0005] The alkaline hydrolysis method is to use d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12P19/12C12P19/14
CPCC12P19/04C12P19/12C12P19/14
Inventor 刘宝龙吴鹏刘晓峰赵岩赵路宋宵因杨少飞
Owner SUZHOU KUNLAN BIOTECH
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