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Technology for cultivating large area of mycorrhizal fungi in Anoectochilus roxburghii(Wall.)Lindl.

A technology of mycorrhizal fungi and clematis, applied in the field of bioengineering, can solve problems such as shortage of clematis resources

Inactive Publication Date: 2018-08-07
石佳媛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for using mycorrhizal fungus to cultivate A. output and quality, to solve the problem of shortage of clematis resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Cultivation of Mushroom MA-1

[0029] After activating MA-1 strains from the slant test tube stored at low temperature once, transfer them to PDA plates, and culture them at a constant temperature of 25°C for 10 days. Use a φ8mm hole puncher to punch holes on the edges of the colonies in the plates to form bacterial flakes, respectively. Into the liquid culture medium with small fragments for fermentation. The composition of the liquid culture medium is calculated by weight percentage: 2.5% of glucose, 0.15% of potassium dihydrogen phosphate, 0.1% of dipotassium hydrogen phosphate, 0.15% of magnesium sulfate, and 3% of wheat bran (cooked juice). The pH of the culture medium is 5.6. 250ml of the culture medium is packed in a 500ml Erlenmeyer bottle, and the MO-1 fungus is inserted after autoclaving. The culture condition is that the shaker shakes at a speed of 120 rpm, and after 14 days of dark culture at 23°C, the bacteria and fermented products are crushed with a h...

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PUM

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Abstract

The invention discloses an application technology for cultivating a large area of mycorrhizal fungi in Anoectochilus roxburghii(Wall.)Lindl. The four mycorrhizal fungi in use are mycena fungi including Mycena dendrobii, Mycenaosmundicola, Mycena orchidicola and Mycena anoectochila; firstly, the mycorrhizal fungi and seedlings of Anoectochilus roxburghii(Wall.)Lindl. are cultivated separately, andwhen the seedlings of Anoectochilus roxburghii(Wall.)Lindl. are transplanted to a field, the mycorrhizal fungi and the seedlings of Anoectochilus roxburghii(Wall.)Lindl. are jointly cultivated. By means of the new technology, the growth period of Anoectochilus roxburghii(Wall.)Lindl. can be greatly shortened, and the yield and the content of effective components of Anoectochilus roxburghii(Wall.)Lindl. are increased. The application technology has a simple cultivation method, large-area popularization is facilitated, the investment is low, chemical fertilizer, hormone and pesticide are not applied at all, and produced Anoectochilus roxburghii(Wall.)Lindl. is a green product.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a technical method for using mycorrhizal fungi in large-area cultivation of clematis to shorten the production cycle of clematis and improve the yield and quality of clematis. Background technique [0002] Clematis is a rare and precious Chinese herbal medicine. It is a perennial herb of the genus Anoectochilus Blume of the family Orchidaceae. It is mainly distributed in Zhejiang, Fujian, Jiangxi, Guangdong, Guangxi, Hainan, Guizhou, Yunnan, Tibet, Taiwan and other provinces and regions. Clematis clematis is a whole herb used as medicine, which has the effects of clearing away heat and cooling blood, expelling wind and dampness, strengthening the heart, lowering blood pressure, detoxifying, relieving pain, and antitussive. Arthritis, infantile convulsions, bruises, snakebites and many other diseases have good curative effects. In recent years, clematis has be...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01H4/00C12N1/14
CPCA01G31/00A01H4/001A01H4/008C12N1/14
Inventor 石佳媛
Owner 石佳媛