Genome-information-assisted breeding method II, breeding parent selection method based on whole-genome SNP (single nucleotide polymorphism) beneficial haplotype mining
An assisted breeding and genomics technology, applied in the fields of genomics, informatics, bioinformatics, etc., can solve problems such as heavy cycle, and achieve the effect of speeding up the breeding process, reducing workload, and narrowing the scope
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[0027] The present invention will be further described below in conjunction with specific implementation examples. The methods used herein are conventional methods unless otherwise specified. The following examples do not limit the invention in any way.
[0028] (1) Genome information acquisition of parental materials
[0029] 1. Test materials
[0030] The breeder's arbitrary candidate breeding parent material.
[0031] 2. DNA extraction and whole genome high-throughput sequencing
[0032] Referring to the DNA extraction method of Temnykh et al. (2000), genomic DNA was extracted from each individual plant.
[0033] Considering the cost, genome sequencing can be based on shot-gun sequencing technology, and the library construction and sequencing methods are the same as conventional. To obtain better quality data coverage, it is recommended to be above 10X. In order to ensure the quality of the sequencing data, if more than 50% of the bases in the original data have a bas...
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