Preparation of polyacrylonitrile modified membrane and method using polyacrylonitrile modified membrane for immobilizing enzyme

A polyacrylonitrile and modified technology, which is applied in the direction of being fixed on/in an organic carrier, can solve the problems of unsupported microorganisms, low gel film strength, low microorganism adhesion, etc., and achieves the effect of excellent performance.

Active Publication Date: 2018-08-17
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] CN1546660A provides a kind of perforated membrane microcapsule carrier for immobilizing microorganisms and its preparation method, using N,N-dimethylformamide as solvent, adopting the mode of surfactant-water coagulation bath to immobilize microorganisms, but its immobilization principle It is adsorption and has low adhesion to microorganisms
The gel film prepared by this method has low strength and is only suitable for embedding some organic macromolecules, but it cannot load microorganisms.

Method used

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  • Preparation of polyacrylonitrile modified membrane and method using polyacrylonitrile modified membrane for immobilizing enzyme
  • Preparation of polyacrylonitrile modified membrane and method using polyacrylonitrile modified membrane for immobilizing enzyme
  • Preparation of polyacrylonitrile modified membrane and method using polyacrylonitrile modified membrane for immobilizing enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] (1) Preparation of polyacrylonitrile hollow membrane

[0059] Prepare polyacrylonitrile hollow membrane by phase inversion method: add 10g polyacrylonitrile powder and 5mL porogen polyethylene glycol into 55mL N,N-dimethylformamide, mix well, seal and react at 65°C for 48h, and form brownish yellow Take out the viscous liquid, cool at room temperature for 12 hours to remove air bubbles; spread the reaction solution in a glass plate groove with a depth of 0.1 mm at room temperature, evaporate at room temperature for 90 seconds, transfer to a coagulation bath (10% DMF / water solution, 25°C) for 2 minutes and cast membrane. Cut the film to 20cm 2 stand-by. Samples were dried and weighed. The porosity of the polyacrylonitrile hollow membrane prepared according to the method is 0.87, and the maximum mechanical tensile force it can bear is 5.89N. The surface morphology of polyacrylonitrile hollow membrane is as follows: figure 1 .

[0060] (2) Preparation of polyacryloni...

Embodiment 2

[0077] Prepare polyacrylonitrile modified membrane and immobilized lipase according to the method of Example 1, the difference is that the calcium chloride is replaced by glutaraldehyde with a concentration of 0.1, 0.2, 0.5, 1.0, 2.0%, to the polypropylene The lipase immobilized on the nitrile-modified membrane was treated, and the treatment conditions were: room temperature, standing still, 90min.

[0078] According to the method, electrostatic adsorption and chemical cross-linking are combined on the surface of the polyacrylonitrile hollow membrane modified membrane to obtain immobilized lipase.

[0079] The surface morphology of the immobilized lipase prepared by the method of Example 2 is as follows: Figure 12 , after repeated use for 15 times, it can retain more than 60% of the enzyme activity ( Figure 9 ), and stored under different conditions (PBS buffer, tap water, sodium chloride, methylene chloride, ethanol, acetone) for 40h, the enzyme activity is better retained...

Embodiment 3

[0081] Prepare polyacrylonitrile modified membrane and immobilized lipase according to the method of Example 1, the difference is that epichlorohydrin with a concentration of 0.1, 0.5, 1.0, 2.5, 5% is used instead of calcium chloride to treat the polyacrylonitrile The lipase immobilized on the acrylonitrile modified membrane was treated, and the treatment conditions were: 37°C, stirring, 90min.

[0082] According to the method, electrostatic adsorption and chemical cross-linking are combined on the surface of the polyacrylonitrile hollow membrane modified membrane to obtain immobilized lipase.

[0083] The surface morphology of the immobilized lipase prepared by the method of Example 3 is as follows: Figure 13 , after repeated use for 15 times, it can retain more than 55% of the enzyme activity ( Figure 9 ), and stored under different conditions (PBS buffer, tap water, sodium chloride, methylene chloride, ethanol, acetone) for 40h, the enzyme activity is better retained ( ...

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Abstract

The invention provides a preparation of a polyacrylonitrile modified membrane and a method using the polyacrylonitrile modified membrane for immobilizing an enzyme; a hollow membrane carrier is prepared from polyacrylonitrile as a raw material in the presence of polyethylene glycol as a pore-foaming agent and N,N-dimethylformamide as a solvent by a L-S phase transformation method; polyamine substance polyethyleneimine is cross-linked on the surface of the polyacrylonitrile hollow membrane carrier by a chemical method, and the enzyme is immobilized by electrostatic adsorption after acidification; in the process of the immobilizing of the enzyme, sodium alginate with biocompatibility is added to increase the immobilized amount of the enzyme and the stability of the immobilized enzyme; finally, different chemical reagents are used to increase the rigidity between the immobilized enzyme and the immobilizing carrier. The immobilizing carrier is obtained by surface modification of a polyacrylonitrile hollow membrane prepared by the phase transformation method, and an electrostatic adsorption immobilization method and an embedding/crosslinking method are combined in the process of the immobilizing of the enzyme to obtain the immobilized enzyme excellent in recyclability. The method is simple in operation, material performance is stable, and the polyacrylonitrile modified membrane hasgood research and industrial application prospects.

Description

technical field [0001] The invention relates to the technical field of enzyme immobilization, in particular to the preparation of a polyacrylonitrile hollow membrane carrier, the modification of polyethyleneimine and its enzyme immobilization application technology. Background technique [0002] Immobilization technology is a biotechnology developed in the middle of last century. Using various methods, the enzyme is immobilized on an insoluble carrier, and the process of preparing an immobilized enzyme is called enzyme immobilization (enzyme immobilization), and an enzyme that is immobilized on a carrier and can catalyze a reaction within a certain space is called For the immobilized enzyme (immobilizedenzyme). Immobilized enzymes have the advantages of low cost, high use efficiency, strong enzyme reusability, and easy separation of products. Based on the above advantages, compared with free enzymes, immobilized enzymes are more suitable for large-scale industrial producti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/36C12N11/10C12N11/08
CPCC08J9/36C08J2201/0422C08J2333/20C12N11/08C12N11/10
Inventor 石贵阳卢佳伟朱惠霖李由然张梁丁重阳顾正华
Owner JIANGNAN UNIV
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