Method for detecting content of trace elements in blood vessels

A technology for medium and trace elements and content, applied in the field of analysis, can solve the problems of high cost, inability to detect multiple elements at the same time, and narrow linear range of atomic absorption spectrometers.

Inactive Publication Date: 2018-08-17
KUNMING UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the method of detecting elements by ultraviolet spectrophotometer is cumbersome, the linear range of atomic absorption spectrometer is narrow and multiple elements cannot be detected at the same time, and the cost of inductively coupled plasma mass spectrometer is high, and it is more suitable for trace element detection

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  • Method for detecting content of trace elements in blood vessels
  • Method for detecting content of trace elements in blood vessels
  • Method for detecting content of trace elements in blood vessels

Examples

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Effect test

Embodiment 1

[0031] A kind of mensuration of phosphorus, calcium, magnesium, iron, zinc element content in descending aorta straight section, concrete steps are as follows:

[0032] (1) Sample treatment: Accurately weigh 0.5g of fresh blood vessel samples in a glass digestion vessel, add 2mL of high-grade pure concentrated nitric acid, and digest in a water bath at 95°C for 5 hours (if the color becomes darker during the digestion process, it will be slightly cold, and an appropriate amount of nitric acid will be added to continue. Digestion), add 0.5mL of hydrogen peroxide dropwise until the solution is light yellow or white and transparent, chase the acid to the remaining 1mL of the solution, cool to room temperature, and dilute to 10mL with 2% nitric acid as the test solution.

[0033] Blank solution preparation: In the digestion vessel without blood vessel sample, carry out digestion under the same conditions with the same concentration of nitric acid and hydrogen peroxide in (1), and t...

Embodiment 2

[0045] A kind of mensuration of phosphorus, calcium, magnesium, iron, zinc element content in the descending aorta bend section, concrete steps are as follows:

[0046] (1) Sample treatment: Accurately weigh 0.8g of a fresh blood vessel sample in a glass digestion vessel, add 3mL of high-grade pure concentrated nitric acid, and digest in a water bath at 93°C for 5.5h (if the color becomes darker during the digestion process, if it is slightly cold, add nitric acid in an appropriate amount Continue to digest), add 0.75mL of hydrogen peroxide dropwise until the solution is light yellow or white and transparent, chase the acid to the remaining 1mL of the solution, cool to room temperature, and dilute to 10mL with 2% nitric acid as the test solution.

[0047] Blank solution preparation: In the digestion vessel without blood vessel sample, carry out digestion under the same conditions with the same concentration of nitric acid and hydrogen peroxide in (1), and the final constant vol...

Embodiment 3

[0059] A kind of mensuration of phosphorus, calcium, magnesium, iron, zinc element content in the straight section of ascending aorta, concrete steps are as follows:

[0060] (1) Sample treatment: Accurately weigh 1.0g of fresh blood vessel sample in a glass digestion vessel, add 4mL of high-grade pure concentrated nitric acid, and digest in a water bath at 90°C for 6 hours (if the color becomes darker during the digestion process and it is slightly cold, add an appropriate amount of nitric acid to continue Digestion), add 1mL hydrogen peroxide dropwise until the solution is light yellow or white transparent, then remove the acid until about 1mL of the solution remains, cool to room temperature, and dilute to 10mL with 2% nitric acid as the test solution.

[0061] Blank solution preparation: In the digestion vessel without blood vessel sample, carry out digestion under the same conditions with the same concentration of nitric acid and hydrogen peroxide in (1), and the final con...

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Abstract

The invention discloses an analytical method for detecting the content of trace elements (phosphorus, calcium, magnesium, iron and zinc) in blood vessels by an inductively coupled plasma emission spectrometer. The method comprises the following steps: performing wet digestion by using a nitric acid-hydrogen peroxide digestion system, establishing a standard curve, and simultaneously detecting thecontent of five trace elements comprising phosphorus, calcium, magnesium, iron and zinc by the inductively coupled plasma emission spectrometer. The method provides an important data analysis and detection basis for exploring the influences of the content of the trace elements on the elasticity of blood vessels and the damage, restoration and apoptosis of vascular endothelial cells in order to further find the interaction law between the trace elements and vascular diseases.

Description

technical field [0001] The invention relates to an analysis method for measuring the content of trace elements (phosphorus, calcium, magnesium, iron and zinc) in blood vessels by using an ICP-OES method, and belongs to the field of analysis technology. Background technique [0002] Blood vessels are channels for transporting blood, and can be divided into arteries, veins, and capillaries according to the direction of transport. The walls of the aorta and aorta are thicker, rich in elastic fibers, and have expandability and elasticity; capillaries have the smallest diameter, the largest number, the largest total cross-sectional area, the slowest blood flow, and the thinnest wall. Composed only of a single layer of endothelial cells and basement membrane, it has good permeability and is conducive to the exchange of substances between blood and tissues; compared with corresponding arteries, veins are larger in number, larger in diameter, thinner in wall, and easy to expand, als...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/73
CPCG01N21/73
Inventor 刘春侠王齐李晓蕾杨俊贺与平周荣锋袁锐波
Owner KUNMING UNIV OF SCI & TECH
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