Astragaloside loaded polycaprolactone membrane and preparation method and applications thereof

A technology of polycaprolactone and astragaloside IV, applied in the field of medicine, can solve the problems of long-term clinical application and side effects, achieve good clinical curative effect, promote recovery, and inhibit exudation

Inactive Publication Date: 2018-08-21
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, drugs for the treatment of corresponding lesions are also used for the treatment of spinal cord injuries, but they are still in the experimental research stage and are still far away from the final clinical application. Even if they are finally applied clinically, they may bring very serious side effects

Method used

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  • Astragaloside loaded polycaprolactone membrane and preparation method and applications thereof
  • Astragaloside loaded polycaprolactone membrane and preparation method and applications thereof
  • Astragaloside loaded polycaprolactone membrane and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Preparation and Characterization of Polycaprolactone Films Loaded with Astragaloside IV

[0059] The preparation of polycaprolactone film loaded with astragaloside includes the following steps: (1) Preparation of polycaprolactone solution: polycaprolactone is put into trifluoroethanol solution, stirred overnight at 37 ° C, and prepared into polycaprolactone Caprolactone solution, wherein the weight-to-volume ratio of polycaprolactone and trifluoroethanol is 10%; (2) preparation of astragaloside solution: astragaloside IV is completely dissolved in dimethyl sulfoxide to make astragaloside A glycoside solution, wherein the mass concentration of the astragaloside IV solution is 0.2g / ml; (3) Preparation of the astragaloside IV polycaprolactone solution: add the astragaloside IV solution to the polycaprolactone solution, and heat the astragaloside IV solution at 37°C Keep stirring until the solution is uniform, wherein the weight-to-volume ratio of astragaloside IV and polyc...

Embodiment 2

[0062] Application of the polycaprolactone film loaded with astragaloside IV prepared by the preparation method in Example 1

[0063] 1. Animal model establishment:

[0064] ① Anesthesia: Calculated at 65 mg / kg, intraperitoneally anesthetized with 1% sodium pentobarbital.

[0065] ②Prepare the rat spinal cord injury model skin in a sterile environment, disinfect with 2% povidone iodine, make an incision about 0.5 cm along the back, incise layer by layer to expose the thoracic 8-thoracic 11 vertebrae, and use microsurgical instruments The lamina of the 9th thoracic vertebra was removed to expose the spinal cord, and the exposed spinal cord was clamped for 1 minute with a special clamp (clamping force of 30g), sterilized, and sutured after placing a biomaterial membrane. The untreated group was directly sterilized and sutured.

[0066] 2. Grouping of experimental animals:

[0067] (1) Divide 42 SD rats of 220-250 g into 3 groups.

[0068] (2) Control group (spinal cord injur...

Embodiment 3

[0080] Preparation of polycaprolactone film loaded with astragaloside IV

[0081] The preparation of polycaprolactone film loaded with astragaloside includes the following steps: (1) preparation of polycaprolactone solution: polycaprolactone is put into trifluoroethanol solution, stirred overnight at 32 ° C, and prepared into polycaprolactone Caprolactone solution, wherein the weight-to-volume ratio of polycaprolactone and trifluoroethanol is 6%; (2) preparation of astragaloside solution: astragaloside IV is completely dissolved in dimethyl sulfoxide to make astragaloside A glycoside solution, wherein the mass concentration of the astragaloside IV solution is 0.3 g / ml; (3) Preparation of the astragaloside IV polycaprolactone solution: add the astragaloside IV solution to the polycaprolactone solution, and heat the astragaloside IV solution at 32° C. Keep stirring until the solution is uniform, wherein the weight-to-volume ratio of astragaloside IV and polycaprolactone solution...

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Abstract

The invention relates to an astragaloside loaded polycaprolactone membrane and a preparation method and applications thereof. The provided astragaloside loaded polycaprolactone membrane can sustained-release drugs, is bio-compatible with spinal cord tissues of rats, can promote the recovery of motor function of damaged spinal cords of rats, inhibits the disintegration of blood-spinal cord barriersof rats, reduces the expression of MMP-9, and prevents the secretion of inflammatory cells. Furthermore, astragaloside can be used in clinic to treat chronic diseases related with kidney; the cost and time for drug development are both reduced; the sustained-release function can increase the local drug concentration, at the same time, the side effect generated by systemic administration is reduced, and the membrane has a clinical application prospect.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a polycaprolactone film loaded with astragaloside IV, a preparation method thereof, and an application thereof in medicine for treating spinal cord injury. Background technique [0002] Spinal cord injury (SCI) is a central nervous system traumatic disease that seriously endangers human survival and quality of life. In recent years, with the rapid development of the transportation industry and the construction industry, the incidence of spinal cord injury has increased year by year, especially among young and middle-aged people, which has brought a huge burden on people's body and mind, family and society. In clinical manifestations, permanent sensory and motor dysfunction of the trunk or limbs below the level of injury is the most common. The severity of its clinical symptoms is related to the level of the damaged segment and the degree of damage. [0003] Clinically, in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/70A61K47/34A61K31/7048A61P19/08
CPCA61K9/7007A61K31/7048A61K47/34A61P19/08
Inventor 张小磊张迪徐华梓田乃锋
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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