A microfluidic detection chip and a kit based on it, whole blood multi-index detection method and application

Abstract

The invention provides a microfluidic detection chip, a kit and a whole blood multi-index detection method based on the same and an application of the microfluidic detection chip. The detection chip comprises two layers, the upper layer is a sample processing layer and the lower layer is a reaction layer, two or more fluid channels are arranged on the surface, facing the reaction layer, of the sample processing layer, and the two layers are closely overlapped to form a closed fluid channel system inside the chip. A plurality of biochemical indexes based on colorimetric measurement, such as liver function, kidney function, blood fat, electrolyte and the like are detected simultaneously in a small test card with a microfluidic chip technology. Difficulties in the application of the existingmicrofluidic technology are overcome, and the chip has low requirements for detection equipment, can be easily applied on site for POCT (point-of-care testing), and is applicable to large, medium andsmall medical institutions, emergency departments, community hospitals, family detection, scientific research and the like.

Description

technical field [0001] The invention belongs to the field of medical detection, and in particular relates to a whole blood detection microfluidic chip and a detection system using the same. Background technique [0002] As we all know, blood is closely related to tissues and organs in various systems of the body, and blood testing is the most extensive sample testing in biomedicine, clinical diagnosis, and health and quarantine diagnosis. At present, most hospitals and laboratories use large centrifuges to separate serum and blood cells, and then use automated biochemical analyzer equipment to detect the contents of various indicators in the blood. However, this method needs to extract a blood sample in milliliters from the patient, and the actual sample used for testing is only a few microliters, resulting in a large waste of samples, and the centrifuge is large in size and complex in structure, and cannot be directly connected to blood testing equipment. As a result, bloo...

AI Technical Summary

Problems solved by technology

However, in this method, blood cells are separated by centrifugation. During the detection process, multiple centrifuges are required to transport the sample to a designated location. The equipment is relatively complicated, which is not conducive to on-site detection.

In addition, during the use of this chip, there is a risk that the diluent will enter the diluent quantitative tank during the sample addition process and enter the mixing tank in advance. This improper operation will cause the chip to fail.

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