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Preparation method of snakehead peptide powder, and snakehead peptide powder

A snakehead peptide and snakehead technology, which is applied in the field of bioactive peptide preparation, can solve the problems that the active ingredients cannot be fully developed and utilized, the protein macromolecular structure cannot be changed, and the urgent needs cannot be met, so as to achieve reasonable amino acid composition and peptide molecular chain Small, reliable results

Inactive Publication Date: 2018-08-31
深圳施之健生命科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the snakeheads currently on the market are still directly heated and eaten or used as medicine, which cannot change the macromolecular structure of the original protein, and its active ingredients cannot be fully developed and utilized.
Although some researchers have tried to enzymatically hydrolyze snakehead meat, there are too few studies on this part, and the effect is mediocre, which cannot meet the urgent demand for small molecule products such as snakehead polypeptides

Method used

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  • Preparation method of snakehead peptide powder, and snakehead peptide powder
  • Preparation method of snakehead peptide powder, and snakehead peptide powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A preparation method of the snakehead peptide powder of the present invention, comprising the following steps:

[0026] The fresh (frozen) snakehead is used as a raw material to carry out pretreatment, cutting and refining, and the gap of the colloid grinding plate used for pulping is 0.5 mm, so as to obtain the snakehead slurry. The Chinese snakehead slurry was precooked at 100°C for 10 minutes, and then papain with an enzyme activity of 600,000 U / g was added to the black snakehead slurry for enzymolysis for 3 hours. The quality of papain was 0.04% of the total mass of the slurry after adding papain, and then Add a compound protease with an enzyme activity of 400,000 U / g for enzymolysis for 4 hours, the mass of the compound protease is 0.03% of the total mass of the slurry after adding the compound protease, and then add a flavor protease with an enzyme activity of 400,000 U / g for enzymolysis for 2 hours , the quality of the flavor protease is 0.04% of the total mass o...

Embodiment 2

[0033] A preparation method of the snakehead peptide powder of the present invention, comprising the following steps:

[0034]The fresh (frozen) snakehead is used as raw material to carry out pretreatment, cutting and refining, and the gap of the colloid mill used for pulping is 0.1mm to obtain the snakehead slurry. Precook the Chinese snakehead slurry at 90°C for 15 minutes, then add papain with an enzyme activity of 600,000 U / g to the black snakehead slurry for 2 hours, and the mass of papain is the total mass of the slurry (including the newly added enzyme, the same below. ), then add a compound protease with an enzyme activity of 400,000 U / g for enzymolysis for 3 hours, the mass of the compound protease is 0.06% of the total mass of the slurry, and then add a flavor protease with an enzyme activity of 400,000 U / g for enzymolysis for 2 hours , the quality of the flavor protease is 0.08% of the total mass of the slurry, and the temperature of the three enzymatic hydrolysis i...

Embodiment 3

[0036] A preparation method of the snakehead peptide powder of the present invention, comprising the following steps:

[0037] The fresh (frozen) snakehead is used as raw material to carry out pretreatment, cutting and refining, and the gap of the colloid mill used for pulping is 0.8 mm to obtain the snakehead slurry. Precook the Chinese snakehead slurry at 110°C for 10 minutes, then add papain with an enzyme activity of 800,000 U / g to the black snakehead slurry for 4 hours, and the mass of papain is the total mass of the slurry (including the newly added enzyme, the same below. ), then add a compound protease with an enzyme activity of 300,000 U / g for enzymolysis for 3 hours. After 3 hours, the mass of the flavor protease was 0.08% of the total mass of the slurry, and the temperatures of the three enzymatic hydrolysis were all 50°C. After the enzymatic hydrolysis is complete, inactivate the enzyme at 95°C for 10 minutes. After inactivating the enzyme, pass through a 40-mesh...

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Abstract

The invention discloses a preparation method of snakehead peptide powder, and the snakehead peptide powder. The preparation method comprises the following steps: carrying out pretreatment, segmentation and pulping on snakehead to obtain snakehead slurry, carrying out pre-boiling, enzymolysis and enzyme deactivation on the snakehead slurry, and then conducting filtration, vacuum concentration and drying to obtain the snakehead peptide powder. The preparation method of the invention overcomes the defects of traditional snakehead using methods, enriches the diversity of snakehead small molecularpeptide products, and is convenient to operate. The prepared snakehead peptide powder is rich in nutrients and easy to digest and absorb, and has wide application prospect and important social value.

Description

technical field [0001] The invention relates to the technical field of preparation of bioactive peptides, in particular to a preparation method of snakehead peptide powder and snakehead peptide powder. Background technique [0002] Snakehead, commonly known as mullet, black fish (North China), raw fish (Guangdong), and financial fish (Hunan, Hubei), is a freshwater fish. The wild meat is thicker and not very tasty, but it has the characteristics of rich nutrition and high protein content. Snakehead meat contains protein, fat, 18 kinds of amino acids, etc., and also contains calcium, phosphorus, iron and multivitamins necessary for the human body. Snakehead also has pharmacological effects such as removing blood stasis and promoting blood circulation, nourishing and aphrodisiac, tightening muscles and promoting body fluid, and recuperating chills. In recent years, there has been an upsurge of research on the health care and medical value of snakehead. However, most of the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/04
CPCA23J1/04
Inventor 向大雄陈勇曹威刘兵夏智胜王建华
Owner 深圳施之健生命科技有限公司