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Colorimetric fluorescent probe capable of quickly and flexibly analyzing bisulfite in high selectivity

A technology of hydrogen atom and sulfonic acid group, which is applied in the field of colorimetric fluorescent probes for rapid, highly selective and sensitive analysis of bisulfite, can solve the problems of complex synthesis, insufficient selectivity, and insufficient response, and achieve simple synthesis Effect

Inactive Publication Date: 2018-09-04
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some problems with the currently reported colorimetric and fluorescent probes, including insufficient selectivity, insufficient response, and complex synthesis.

Method used

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  • Colorimetric fluorescent probe capable of quickly and flexibly analyzing bisulfite in high selectivity
  • Colorimetric fluorescent probe capable of quickly and flexibly analyzing bisulfite in high selectivity
  • Colorimetric fluorescent probe capable of quickly and flexibly analyzing bisulfite in high selectivity

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]

[0035]Dissolve 300mg of fluorophore compound and levulinic acid (270mg) in dichloromethane (15mL), stir at room temperature under the action of 4-dimethylaminopyridine (75mg) and dicyclohexylcarbodiimide (255mg) After 6 hours, the product was observed by spotting the plate, and the solution was suction filtered to obtain a filter cake, which was separated by column chromatography with a mixed system of dichloromethane and methanol (v / v, 100:1) to obtain about 230 mg of pure product. The yield was 61%.

[0036] 1 H NMR verified the correctness of the product structure.

Embodiment 2

[0038] Figure 1(a) is the probe (5μM) added HSO 3 - Fluorescence spectra before and after (0-70μM), specifically, prepare 100mL of 5μM probe solution, fixedly dissolve it in seven 10mL colorimetric tubes, then add different concentrations of bisulfite, and measure after 40min of response. From the figure, we can see that as the concentration of bisulfite increases, the fluorescence spectrum of the probe increases gradually, and the fluorescence change is very obvious. Figure 1(b) is the probe for different concentrations of HSO 3 - (0-50μM) response fluorescence spectrum, and its good linear relationship indicates that the probe can accurately quantify bisulfite.

Embodiment 3

[0040] figure 2 Yes probe (20 μM) added HSO 3 - The absorption spectrum before and after (100 μM) is specifically: prepare 10 mL of probe with a concentration of 20 μM and divide it into two, add 100 μM bisulfite to one of the colorimetric tubes, shake well, and measure. It can be seen from the figure that the absorbance increases significantly before and after the reaction.

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Abstract

The invention relates to a colorimetric fluorescent probe capable of quickly and flexibly analyzing bisulfite in high selectivity. Specifically, the probe disclosed by the invention is acetoacetic ester compound and can serve as a bisulfite colorimetric fluorescent probe to be applied to bisulfite detection. The probe has at least one of the following technical effects of recognizing bisulfite inhigh selectivity, achieving response to bisulfite, having simpleness in preparation, achieving analysis on the bisulfite, having stable character, being stored and used for a long time and having stronger antijamming capability.

Description

technical field [0001] The invention relates to an acetoacetate compound as a bisulfite fluorescent probe, which can quickly and highly selectively identify bisulfite, or it can measure the concentration of bisulfite in a sample. Background technique [0002] Sulfur dioxide (SO 2 ) has been considered as a serious environmental pollutant in the past few years, which can endanger human health, cause acid rain, and harm aquatic animals. Its derivative (bisulfite) prevents mold due to its ability to inhibit the growth of microorganisms, so bisulfite is used as a preservative and antioxidant in food, wine and pharmaceuticals. Also, high levels of sulfites can cause diarrhea, low blood pressure, allergies, and asthma. In the process of modern industrial production, factories release a large amount of bisulfite, which can cause serious environmental problems. Some studies have also reported that bisulfite produced in the cytosol and mitochondria can regulate cardiovascular stru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/08C09K11/06G01N21/64G01N21/78
CPCC07D493/08C09K11/06C09K2211/1088G01N21/643G01N21/78
Inventor 祝汉闯段庆霞柳彩云李子璐贾盼朱宝存
Owner UNIV OF JINAN