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Process for eliminating trifluoroacetic acid in coenzyme I

A technology of trifluoroacetic acid and coenzyme, which is applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., can solve the problems of high residue and poor removal effect, and achieve a long process, fast moving speed, and easy removal. Effect

Inactive Publication Date: 2018-09-04
开封康诺药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing removal process has been limited by conditions, the removal effect is not good, and the residue is high

Method used

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  • Process for eliminating trifluoroacetic acid in coenzyme I
  • Process for eliminating trifluoroacetic acid in coenzyme I
  • Process for eliminating trifluoroacetic acid in coenzyme I

Examples

Experimental program
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Effect test

Embodiment 1

[0044]Sephadex G25 was mixed with purified water for swelling. A glass column with an inner diameter of 5 cm and a height of 100 cm was used as a chromatography column, and the swollen Sephadex G25 was loaded into the chromatography column, and the Sephadex G25 was filled with a total of 1.6 L. Then the coenzyme I with a volume concentration of 40% is delivered to the chromatography column by a diaphragm pump at a feed rate of 18mL / min for chromatographic separation, and the eluent is delivered to the chromatography column at 8L / h for elution. At the same time, the ultraviolet detector is used to monitor the liquid after chromatographic separation, and the eluate is collected at the same time as the detection. When the reading of the protein nucleic acid meter tends to be stable, the sample can be collected for testing. Wherein, the total feed amount of coenzyme I is 50mL.

Embodiment 2

[0046] Sephadex G25 was mixed with purified water for swelling. A glass column with an inner diameter of 5 cm and a height of 110 cm was used as a chromatography column, and the swollen Sephadex G25 was loaded into the chromatography column, and the Sephadex G25 was filled with a total of 1.5 L. Then the coenzyme I with a volume concentration of 38% is delivered to the chromatography column by a diaphragm pump at a feed rate of 20mL / min for chromatographic separation, and the eluent is delivered to the chromatography column at 8L / h for elution. At the same time, the protein and nucleic acid instrument is used to monitor the liquid after chromatographic separation, and the eluate is collected while detecting. When the reading of the ultraviolet detector tends to be stable, the sample can be collected for testing. Wherein, the total feed amount of coenzyme I is 50mL.

Embodiment 3

[0048] Sephadex G25 was mixed with purified water for swelling. A glass column with an inner diameter of 5 cm and a height of 100 cm was used as a chromatography column, and the swollen Sephadex G25 was loaded into the chromatography column, and the Sephadex G25 was filled with a total of 1.7 L. Then the coenzyme I with a volume concentration of 42% is delivered to the chromatographic column by a diaphragm pump at a feed rate of 16mL / min for chromatographic separation, and the eluent is delivered to the chromatographic column at 7L / h for elution. At the same time, the protein and nucleic acid instrument is used to monitor the liquid after chromatographic separation, and the eluate is collected while detecting. When the reading of the ultraviolet detector tends to be stable, the sample can be collected for testing. Wherein, the total feed amount of coenzyme I is 50mL.

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Abstract

The invention provides a process for eliminating trifluoroacetic acid in coenzyme I, and relates to the technical field of medicine separation. The process for eliminating trifluoroacetic acid in coenzyme I comprises the following steps of using sephadex as chromatography filling materials for eliminating trifluoroacetic acid in coenzyme I through chromatography. The process can be used for effectively eliminating trifluoroacetic acid in coenzyme I; the removal effect is good.

Description

technical field [0001] The invention relates to the technical field of drug separation, and in particular to a process for removing trifluoroacetic acid in coenzyme I. Background technique [0002] Trifluoroacetic acid (TFA) is a strong carboxylic acid with pKa=0.23, which can irritate human tissues and skin. Although slightly toxic, enrichment in stagnant surface water will affect agriculture and aquatic systems, and trifluoroacetic acid undergoes microbial degradation to produce greenhouse gas CHF3. When it reaches a certain concentration, it will cause harm to the human body. [0003] Therefore, the process of removing trifluoroacetic acid is particularly important and urgent. However, the existing removal process has always been limited by conditions, and the removal effect is not good and the residue is high. SUMMARY OF THE INVENTION [0004] The object of the present invention is to provide a kind of technology that removes trifluoroacetic acid in coenzyme I, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H19/207C07H1/06
CPCC07H1/06C07H19/207
Inventor 金永红郎巧洁贾和平
Owner 开封康诺药业有限公司