A kind of moringa enzymatic hydrolyzate and its preparation method and application
A technology of moringa enzyme and enzymatic hydrolyzate, which is applied in the preparation of moringa enzymatic hydrolyzate and the field of moringa enzymatic hydrolyzate, can solve the problems of poor efficacy, lagging development of moringa oleifera, low absorption efficiency of moringa oleifera leaves, etc., and achieve Low production cost, reduced post-processing steps, and high oral bioavailability
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[0027]The present invention provides a preparation method of Moringa oleifera enzymatic hydrolysate, which comprises the following steps:
[0028]a. Mix 100 parts by weight of Moringa oleifera raw material with 800-1200 parts by weight of water, add 1-8 parts of carbohydrase to the mixture, enzymatically hydrolyze at a constant temperature of 30-65°C for 0.5-12 hours to obtain a carbohydrate hydrolysate;
[0029]b. Adjust the pH value of the carbohydrate enzymatic hydrolysate to 8-9, add 1 to 8 parts by weight of alkaline protease to the carbohydrate hydrolysate, and conduct constant temperature enzymatic hydrolysis at 30-65°C for 0.5-12 hours to obtain primary Proteolysis product
[0030]c. Add 1-8 parts by weight of neutral protease to the primary proteolysate obtained in step b, and enzymatically hydrolyze at 30-65°C for 0.5-12 hours to obtain the final proteolysate;
[0031]d. Inactivate the enzymes in the final proteolytic hydrolysate in step c, and obtain the Moringa oleifera hydrolysate ...
Embodiment 1
[0052]Preparation of Moringa Leaf Enzymatic Hydrolysate from Yunnan Dry Moringa Leaf
[0053]Crush 100 grams of clean Moringa leaves from Yunnan, add 1000 ml of deionized water at a constant temperature of 55°C, adjust the pH to 5, add 2 grams of carbohydrase, and react for 3 hours; then at the same temperature, adjust the pH with sodium hydroxide solution To 8, add 2 grams of alkaline protease to react for 2 hours under constant temperature stirring; then add 2 grams of neutral protease to react for 2 hours under the same temperature condition; heat the obtained enzymatic hydrolysate to 90°C and keep the enzyme at constant temperature for half an hour to deactivate the enzyme Live, filter. The filtrate was concentrated under reduced pressure at a vacuum of 0.08Mpa and a temperature of 50°C, and finally lyophilized to obtain a solid dry powder of Moringa oleifera leaf enzymatic hydrolysate with a yield of 62.1%.
[0054]After determination, the obtained dry powder of Moringa oleifera leaf...
Embodiment 2
[0059]Preparation of Moringa oleifera leaf enzymatic hydrolysate dry powder from Sichuan Moringa leaf
[0060]Add 100 g of clean dried Moringa leaves from Sichuan, add 800 ml of deionized water at 35°C, adjust the pH to 5, add 7 g of carbohydrase, and react for 4 hours; then adjust the pH to 8 at the same temperature Add 8 grams of alkaline protease to react for 5 hours under constant temperature stirring; then add 8 grams of neutral protease to react for 5 hours under the same temperature condition; then heat to 80°C, react at constant temperature for 40 minutes to inactivate the enzyme and filter. The filtrate was concentrated under reduced pressure at a vacuum degree of 0.10Mpa and a temperature of 40°C, and finally lyophilized to obtain a solid dry powder of Moringa oleifera leaf enzymatic hydrolysate with a yield of 48.5%.
[0061]After determination, the obtained Moringa oleifera leaf enzymatic hydrolysate dry powder: crude protein content 35%, total amino acid content: 31.33%; of w...
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