A kind of moringa enzymatic hydrolyzate and its preparation method and application

A technology of moringa enzyme and enzymatic hydrolyzate, which is applied in the preparation of moringa enzymatic hydrolyzate and the field of moringa enzymatic hydrolyzate, can solve the problems of poor efficacy, lagging development of moringa oleifera, low absorption efficiency of moringa oleifera leaves, etc., and achieve Low production cost, reduced post-processing steps, and high oral bioavailability

Active Publication Date: 2020-12-29
成都金希生态农业开发股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the development of Moringa products in my country is relatively lagging behind. The functional foods on the market are mainly rough processed directly by crushing Moringa leaves. The absorption efficiency of Moringa leaves is low and the efficacy is not good.
Moreover, most of the current research on Moringa leaves is to extract flavonoids, and the research on polysaccharides, alkaloids, water-soluble proteins and other components and their active components is rarely seen.

Method used

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  • A kind of moringa enzymatic hydrolyzate and its preparation method and application
  • A kind of moringa enzymatic hydrolyzate and its preparation method and application

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preparation example Construction

[0027]The present invention provides a preparation method of Moringa oleifera enzymatic hydrolysate, which comprises the following steps:

[0028]a. Mix 100 parts by weight of Moringa oleifera raw material with 800-1200 parts by weight of water, add 1-8 parts of carbohydrase to the mixture, enzymatically hydrolyze at a constant temperature of 30-65°C for 0.5-12 hours to obtain a carbohydrate hydrolysate;

[0029]b. Adjust the pH value of the carbohydrate enzymatic hydrolysate to 8-9, add 1 to 8 parts by weight of alkaline protease to the carbohydrate hydrolysate, and conduct constant temperature enzymatic hydrolysis at 30-65°C for 0.5-12 hours to obtain primary Proteolysis product

[0030]c. Add 1-8 parts by weight of neutral protease to the primary proteolysate obtained in step b, and enzymatically hydrolyze at 30-65°C for 0.5-12 hours to obtain the final proteolysate;

[0031]d. Inactivate the enzymes in the final proteolytic hydrolysate in step c, and obtain the Moringa oleifera hydrolysate ...

Embodiment 1

[0052]Preparation of Moringa Leaf Enzymatic Hydrolysate from Yunnan Dry Moringa Leaf

[0053]Crush 100 grams of clean Moringa leaves from Yunnan, add 1000 ml of deionized water at a constant temperature of 55°C, adjust the pH to 5, add 2 grams of carbohydrase, and react for 3 hours; then at the same temperature, adjust the pH with sodium hydroxide solution To 8, add 2 grams of alkaline protease to react for 2 hours under constant temperature stirring; then add 2 grams of neutral protease to react for 2 hours under the same temperature condition; heat the obtained enzymatic hydrolysate to 90°C and keep the enzyme at constant temperature for half an hour to deactivate the enzyme Live, filter. The filtrate was concentrated under reduced pressure at a vacuum of 0.08Mpa and a temperature of 50°C, and finally lyophilized to obtain a solid dry powder of Moringa oleifera leaf enzymatic hydrolysate with a yield of 62.1%.

[0054]After determination, the obtained dry powder of Moringa oleifera leaf...

Embodiment 2

[0059]Preparation of Moringa oleifera leaf enzymatic hydrolysate dry powder from Sichuan Moringa leaf

[0060]Add 100 g of clean dried Moringa leaves from Sichuan, add 800 ml of deionized water at 35°C, adjust the pH to 5, add 7 g of carbohydrase, and react for 4 hours; then adjust the pH to 8 at the same temperature Add 8 grams of alkaline protease to react for 5 hours under constant temperature stirring; then add 8 grams of neutral protease to react for 5 hours under the same temperature condition; then heat to 80°C, react at constant temperature for 40 minutes to inactivate the enzyme and filter. The filtrate was concentrated under reduced pressure at a vacuum degree of 0.10Mpa and a temperature of 40°C, and finally lyophilized to obtain a solid dry powder of Moringa oleifera leaf enzymatic hydrolysate with a yield of 48.5%.

[0061]After determination, the obtained Moringa oleifera leaf enzymatic hydrolysate dry powder: crude protein content 35%, total amino acid content: 31.33%; of w...

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Abstract

The invention discloses horseradish tree zymolyte as well as a preparation method and application thereof and relates to the technical field of biological extraction. According to the horseradish treezymolyte disclosed by the invention, a horseradish tree raw material is resolved by carbohydrase at first, horseradish tree cell walls are broken, and other matters in the cells are released; then protease is used for degrading plant protein in the horseradish tree cells into small-molecular polypeptide; zymolyte obtained by degradation can be directly applied to drying after enzyme deactivationand filtering to obtain horseradish tree zymolyte; thus, a preparation technology is simple and convenient, and production cost is low. The prepared horseradish tree zymolyte can extract protein and polysaccharide in the horseradish trees to the maximum extent, can fully gather biological active small molecules, is easy to digest and absorb in the human body and has high bioavailability and a goodanti-fatigue effect.

Description

Technical field[0001]The invention relates to the technical field of biological extraction, in particular to a preparation method of Moringa oleifera enzymatic hydrolysate, the prepared Moringa enzymatic hydrolysate and application.Background technique[0002]Moringa is a perennial tropical and subtropical deciduous tree. It belongs to the Moringa genus of Moringa family in the quilt door. Its Latin scientific name: Moringa oleifera Lam., English name: Moringa. There are also various aliases. Triangle), called the Drumstick tree, which is called the Horseradish tree (Horseradish tree) according to the pungent taste of its roots, and called the Ben oil tree or Benzoil tree (Ben oil tree or Benzoil tree) according to its seeds. . There are about 14 species of Moringa trees in the world. They can grow naturally outdoors in tropical or subtropical climates with an altitude of 0-2000 meters and an annual rainfall of 800-3000 mm. The soil conditions are relatively wide, and the pH value is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C12P19/14C12P19/04A23L33/00A61K36/185A61P39/00
CPCA23V2002/00A61K36/185A23L33/00A61P39/00C12P19/04C12P19/14C12P21/06A23V2200/30
Inventor 史宗洁张思良张涛朱筱莉周玮黄萍叶仁礼张军郭黄勋张晓凤
Owner 成都金希生态农业开发股份有限公司
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