Compositions and methods for healing wounds
A composition and wound technology, applied in the direction of drug combination, tissue regeneration, medical science, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0097] Embodiment 1: prepare pigskin skeleton
[0098] Gal / gal pig skin was purchased from Avantea (Cremona, Italy). A piece of skin measuring 20 x 15 cm was dissected from a Gal / gal knockout pig, vacuum sealed into a plastic bag and frozen at -200°C until use. Pig skin was thawed at room temperature, cleaned by excision of subcutaneous fat tissue, hair removed, and finally rinsed with distilled water. The skin was placed in a 5 L plastic container with 0.5% SDS (Sodium Lauryl Sulfate) (Sigma, Germany) containing 0.02% Sodium Azide (Sigma, Germany) and 1.86% EDTA (Alfa Aesar, Germany). 37°C, 200 RPM continuous shaking for 9 days. The SDS is replaced first after 24 hours and then every 48 hours. Each time the SDS was replaced, the skin was washed with distilled water for 1 h.
[0099] Decellularized porcine skin
[0100] Gal / gal knockout pig skin was completely decellularized after 9 days of continuous treatment with 0.5% SDS. Figures 1A to 1G Porcine skin is shown before...
Embodiment 2
[0127] Example 2: Effect of Porcine Skin Gel (PSG) and Human Peripheral Blood Mononuclear Cells (hPBMC) in Full Thickness Wound Healing
[0128] To determine the wound-healing capacity of PSG, acute full-thickness incision wounds made on the backs of nude mice were treated with no treatment or with HA, PSG alone or PSG+hPBMCs. image 3 The wound healing process of untreated wounds and wounds treated with PSG only or PSG+hPBMCs is shown. On postoperative days 5 and 10, scab formation was observed in all groups. However, at day 15, the scab flaked off in the PSG-treated group and the wound was filled with regenerated skin.
[0129] Wound closure in animals treated with PSG only and PSG+hPBMC
[0130] A clear difference was observed with respect to wound closure in animals treated with PSG and PSG+hPBMC only. On day 15, wounds in these animals exhibited the most pronounced difference in wound closure. On day 15, 5 / 6 (83%, p image 3 As shown, the difference between the control...
Embodiment 3
[0140] Example 3: Human and Host Vascularization of Wounds Accelerates wound healing by promoting neovascularization.
[0141] CD31 staining on postoperative days 5 and 10 showed vascularization in each experimental group. A large number of host (mouse) microvessels can be seen in the PSG+hPBMC treatment group on the 5th and 10th day, and compared with the control group, the microvessel density is much higher (corresponding to Figure 7A , 7D , and 7G). Importantly, many small vessels were positively stained in the PSG+hPBMC-treated group at day 5 when stained with a human-specific anti-CD31 antibody ( Figure 7B ). However, the number of blood vessels positively stained for human CD31 decreased on the 10th day ( Figure 7E ) and is negligible on day 15. This finding indicates that human PBMCs accelerate wound healing by promoting neovascularization.
PUM
| Property | Measurement | Unit |
|---|---|---|
| width | aaaaa | aaaaa |
| thickness | aaaaa | aaaaa |
| length | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More