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Anionic porphyrin-carbon nanotube modified electrode for measuring the specific sequence of her2 gene

A specific sequence, modified electrode technology, applied in the field of biosensing and nanomaterials, can solve the problems of expensive and time-consuming, and achieve the effect of high sensitivity, good anti-interference, and excellent electrochemical response.

Inactive Publication Date: 2020-07-24
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
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Problems solved by technology

[0002] The proto-oncogene human epidermal growth factor receptor-2 (human epidermal growth factor receptor-2, HER2) gene is a prognostic factor for breast cancer. Therefore, it is very important to quickly and accurately detect HER2 and determine its status for the selection of treatment options for breast cancer patients. The methods for clinical detection of HER2 include immunochemistry (IHC) and fluorescence in situ hybridization (FISH). These two methods require biopsy sample preparation, professional operations, expensive instruments, and time-consuming disadvantages. Therefore, it is easy to construct a , rapid, sensitive and accurate detection of HER2 is of great significance

Method used

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  • Anionic porphyrin-carbon nanotube modified electrode for measuring the specific sequence of her2 gene
  • Anionic porphyrin-carbon nanotube modified electrode for measuring the specific sequence of her2 gene
  • Anionic porphyrin-carbon nanotube modified electrode for measuring the specific sequence of her2 gene

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example 1

[0022] Example 1: A method and application for detecting the specific sequence of the HER2 gene based on an anionic porphyrin-carbon nanotube modified electrode: use 0.3 μm, 0.05 μm α-Al for GCE 2 o 3 Polish to a mirror surface, then ultrasonically clean with ethanol and double distilled water respectively, and dry at room temperature. Ultrasonic dispersion of carbon nanotubes in 0.05 M, pH = 8.00 Tris-HCl containing 0.1 M KCl buffer to obtain a CNTs dispersion; so that the subsequently added anionic porphyrin (TSPP) solution cannot approach the surface of CNTs; the final addition needs to be detected The target DNA (Target DNA, tDNA) hybridized with pDNA to form double-stranded DNA (dsDNA) detached from the surface of CNTs. At this time, TSPP can be adsorbed on the surface of CNTs through π-π interaction. Finally, the buffer solution was drop-coated on the electrode surface to obtain modified electrodes, namely: TSPP / pDNA / CNTs / GCE and TSPP / dsDNA / CNTs / GCE.

example 2

[0023] Example 2 A method and application for detecting the specific sequence of the HER2 gene based on an anionic porphyrin-carbon nanotube modified electrode prepared by the present invention.

[0024] (1) Qualitative detection of DNA by different modified electrodes

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Abstract

The invention relates to detection of a specific sequence of a human epidermal growth factor receptor-2 (HER2) gene by means of a porphyrin / carbon nanomaterial modified electrode. The detection comprises the steps of firstly, ultrasonically dispersing carbon nanotubes in a buffer solution, and then sequentially adding probe DNA and porphyrin, wherein at the moment, only the probe DNA can be woundon the surfaces of the carbon nanotubes under the action of pi-pi; adding target DNA needing to be detected, and enabling the target DNA and the probe DNA to be hybridized into double strands fallingoff from the surfaces of the carbon nanotubes, so as to enable the porphyrin to be adsorbed on the surfaces of the carbon nanotubes. An impedance change value of the modified electrode is detected byusing an alternating current impedance method according to the change of surface-loaded substances of the carbon nanotubes before and after the hybridization, so that the detection of the target DNA is realized. The HER2 sequence detection linear range of the modified electrode is 2.0*10<-11>-2.0*10<-6>M, and the detection limit is 6.34 multiplied by 10<-11>M (S / N=3). An electrochemical sensor iseasy to prepare, has high sensitivity, does not need to be labeled, and is expected to be popularized and applied in the field of medical diagnosis.

Description

technical field [0001] The invention designs a method for constructing an ultrasensitive electrochemical DNA sensor for the quantitative detection of a specific sequence of the HER2 gene, and relates to a method for detecting an anionic porphyrin-carbon nanotube material modified electrode for human epidermal growth factor receptor HER2 and applications, belonging to the technical field of biosensing and nanomaterials. Background technique [0002] The proto-oncogene human epidermal growth factor receptor-2 (human epidermal growth factor receptor-2, HER2) gene is a prognostic factor for breast cancer. Therefore, it is very important to quickly and accurately detect HER2 and determine its status for the selection of treatment options for breast cancer patients. The methods for clinical detection of HER2 include immunochemistry (IHC) and fluorescence in situ hybridization (FISH). These two methods require biopsy sample preparation, professional operations, expensive instrument...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/327
CPCG01N27/26G01N27/3278
Inventor 宁静恒刘龙王敏罗新
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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