A class of isopimarane-type diterpene derivatives, its pharmaceutical composition and its use
A technology of isopimarane and derivatives, applied in pharmaceutical compositions for treating estrogen-related diseases, applications in medicines, and the field of estrogen biosynthesis regulators, can solve the problem of no pharmaceutical composition, no isopimarane diterpene derivatives things, etc.
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Embodiment 1
[0047] Preparation of isopimarane-type diterpene derivatives (1-7) (I):
[0048] Extraction and separation of isopimarane-type diterpene derivatives (1–7)(I):
[0049] The semi-preparative HPLC used in the experiment is Agilent 1260 and Zorbax SB-C18 (9.4mm × 25cm) chromatographic column; thin-layer chromatography silica gel, column chromatography silica gel (100-200 mesh and 200-300 mesh) were purchased from Qingdao Mei High Group Ltd.; reverse C18 silica gel was Lichroprep RP-18gel (40-63 μm, Merck, Darmstadt, Germany), and Sephadex LH-20 (Pharmacia) was used for Sephadex.
[0050] Collect the leaf branches of Premnafulva, dry, crush, extract three times with 80% ethanol at room temperature, combine the ethanol extracts, filter, and concentrate under reduced pressure; suspend the extract in aqueous solution, and wash with ethyl acetate After extraction, the ethyl acetate extraction part was concentrated under reduced pressure, dissolved and adsorbed on silica gel with chlor...
Embodiment 2
[0132] According to the method of Example 1, the isopimarane-type diterpene derivatives (1-7) were firstly prepared, dissolved with a small amount of DMSO respectively, added water for injection as usual, finely filtered, potted and sterilized to make an injection.
Embodiment 3
[0134] According to the method of Example 1, the isopimarane type diterpene derivative (1-7) was first prepared, and after dissolving with a small amount of DMSO, it was dissolved in sterile water for injection, stirred to dissolve, and filtered through a sterile suction funnel Filtration, and then aseptic fine filtration, sub-package in ampoules, freeze-drying at low temperature and then aseptically melt-seal to obtain powder injection.
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