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Method for purifying and separating bacterial cellulose

A technology of bacterial cellulose and separation method, applied in the field of cellulose purification and separation, can solve the problems of long purification cycle and low efficiency, and achieve the effects of shortening the purification and separation cycle, improving purification efficiency and high purity of bacterial cellulose.

Inactive Publication Date: 2018-09-14
BEIJING INST OF TECH ZHUHAI CAMPUS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing purification and separation methods for bacterial cellulose have a long purification cycle and low efficiency.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A kind of purification and separation method of bacterial cellulose proposed by the present invention comprises the following steps:

[0026] S1. Put the bacterial cellulose wet film into distilled water, and then heat it to boiling for 4-16 minutes to sterilize the bacterial cellulose;

[0027] S2. Take out the bacterial cellulose wet film in step S1, put it into a sodium hydroxide solution with a pH of 11, and take it out after soaking at a constant temperature of 35° C. for 3 to 9 minutes under the action of a constant temperature magnetic stirrer;

[0028] S3. Put the bacterial cellulose wet film obtained in step S2 into a hydrochloric acid solution with a pH of 4, and soak it at a constant temperature of 35° C. for 4 to 8 minutes under the stirring action of a constant temperature magnetic stirrer at 150 to 650 rpm;

[0029] S4, centrifuging the bacterial cellulose wet film obtained in step S3, removing the precipitate, and obtaining the bacterial cellulose superna...

Embodiment 2

[0035] A kind of purification and separation method of bacterial cellulose proposed by the present invention comprises the following steps:

[0036] S1. Put the bacterial cellulose wet film into distilled water, and then heat it to boiling for 4-16 minutes to sterilize the bacterial cellulose;

[0037] S2. Take out the bacterial cellulose wet film in step S1, put it into a sodium hydroxide solution with a pH of 11, and take it out after soaking at a constant temperature of 35° C. for 3 to 9 minutes under the action of a constant temperature magnetic stirrer;

[0038] S3. Put the bacterial cellulose wet film obtained in step S2 into a hydrochloric acid solution with a pH of 4, and soak it at a constant temperature of 35° C. for 4 to 8 minutes under the stirring action of a constant temperature magnetic stirrer at 150 to 650 rpm;

[0039] S4, centrifuging the bacterial cellulose wet film obtained in step S3, removing the precipitate, and obtaining the bacterial cellulose superna...

Embodiment 3

[0045] A kind of purification and separation method of bacterial cellulose proposed by the present invention comprises the following steps:

[0046] S1. Put the bacterial cellulose wet film into distilled water, and then heat it to boiling for 4-16 minutes to sterilize the bacterial cellulose;

[0047] S2. Take out the bacterial cellulose wet film in step S1, put it into a sodium hydroxide solution with a pH of 9, and take it out after soaking at a constant temperature of 35° C. for 3 to 9 minutes under the action of a constant temperature magnetic stirrer;

[0048] S3. Put the bacterial cellulose wet film obtained in step S2 into a hydrochloric acid solution with a pH of 4, and soak it at a constant temperature of 35° C. for 4 to 8 minutes under the stirring action of a constant temperature magnetic stirrer at 150 to 650 rpm;

[0049] S4, centrifuging the bacterial cellulose wet film obtained in step S3, removing the precipitate, and obtaining the bacterial cellulose supernat...

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PUM

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Abstract

The invention discloses a method for purifying and separating bacterial cellulose. The method comprises the following steps: placing a bacterial cellulose wet film into distilled water, heating to boiling for 4-16min, then taking out the bacterial cellulose wet film, putting the bacterial cellulose wet film into an alkali solution with a pH value of 9-12, soaking for 3-9min and then taking out, putting the bacterial cellulose wet film into an acid solution with a pH value of 3-6, and soaking for 4-8min; then, centrifuging the bacterial cellulose wet film to obtain bacterial cellulose supernatant; adding mixed liquid of n-butanol and chloroform into the supernatant according to a ratio of 1 to (0.2-1), and extracting for 3-6 times in water bath at the constant temperature of 20-60 DEG C toobtain a protein removal solution; adding an organic solvent as an extractant into the protein removal solution according to a ratio of 1 to (0.22-1) for extracting organic small molecules so as to obtain organic removal liquid; enabling the organic removal liquid to pass through a silica gel chromatographic column and be subjected to column chromatography so as to obtain a bacterial cellulose solution; washing for 2-3 times and then storing in a refrigerator at 8-15 DEG C. The method is high in purification and separation degree, short in period and high in efficiency.

Description

technical field [0001] The invention relates to the technical field of cellulose purification and separation, in particular to a method for the purification and separation of bacterial cellulose. Background technique [0002] Cellulose is a macromolecular polysaccharide composed of glucose, insoluble in water and general organic solvents, and is the main component of plant cell walls. Cellulose is the most widely distributed and most abundant polysaccharide in nature, accounting for more than 50% of the carbon content in the plant kingdom. The cellulose content of cotton is close to 100%, making it the purest natural source of cellulose. In general wood, cellulose accounts for 40-50%, and there are 10-30% hemicellulose and 20-30% lignin. Bacterial cellulose refers to the general term for cellulose synthesized by certain microorganisms in the genus Acetobacter, Agrobacterium, Rhizobium and Sarcina under different conditions. Bacterial cellulose and natural cellulose produc...

Claims

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Application Information

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IPC IPC(8): D21C5/00
CPCD21C5/00
Inventor 王磊欧阳文璟矫庆泽鲁敏王广胜关晓辉林楚宏蔡阳伦郭冰之
Owner BEIJING INST OF TECH ZHUHAI CAMPUS
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