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Preparation method of multiple nanoemulsion vaccine adjuvant

A vaccine adjuvant and nanoemulsion technology, applied in the field of vaccine adjuvant preparation, can solve the problems of unsatisfactory stability of ISA206 adjuvant, unreasonable formula ingredients, preparation process, low immunity, etc., to enhance immune cells Proliferation ability, good emulsion stability, good absorption effect

Active Publication Date: 2022-02-11
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantage is that the stability of the prepared ISA206 adjuvant is not satisfactory, and the particle size is relatively large, belonging to the micron level
[0006] So far, there have been few reports on the development of multiple nanoemulsion vaccine adjuvants, which is closely related to the difficulty of its development process. Due to the unreasonable formulation components and preparation process of multiple nanoemulsion vaccine adjuvants in the prior art, there are still injections during use. Difficult, less immune deficiencies

Method used

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  • Preparation method of multiple nanoemulsion vaccine adjuvant
  • Preparation method of multiple nanoemulsion vaccine adjuvant
  • Preparation method of multiple nanoemulsion vaccine adjuvant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A multiple nanoemulsion vaccine adjuvant, which is composed of an inner water phase, an intermediate oil phase and an outer water phase;

[0037] The components in the internal aqueous phase, calculated on the total weight of the water-in-oil-in-water vaccine adjuvant raw materials, are in a proportion of: 50.0 mg / mL lentinan injection 2.0%;

[0038] The components in the intermediate oil phase, based on the total weight of the water-in-oil-in-water vaccine adjuvant raw materials, have the following proportions: 1.2% lecithin, 1.2% ethanol, 2.8% squalene, and 2.8% palm oil;

[0039] The components in the external aqueous phase, based on the total weight of the water-in-oil-in-water vaccine adjuvant raw materials, are in proportions: Tween-80 3.7%, Span-80 3.7%, and the balance is normal saline.

[0040] The preparation method of the above-mentioned multiple nanoemulsion vaccine adjuvant comprises the following steps;

[0041] 1) Dissolving the immune enhancer in physiolo...

Embodiment 2

[0048] A multiple nanoemulsion vaccine adjuvant consisting of an inner water phase, an intermediate oil phase and an outer water phase;

[0049] The components in the internal water phase, based on the total weight of the water-in-oil-in-water type vaccine adjuvant raw materials, the ratio is: 50.0mg / mL lentinan injection 0.5%;

[0050] Each component in the middle oil phase, based on the total weight of the water-in-oil-in-water type vaccine adjuvant raw materials, has a ratio of: 0.8% lecithin, 0.8% ethanol, 3.2% squalene, and 3.2% palm oil;

[0051] Each component in the external water phase, based on the total weight of the water-in-oil-in-water type vaccine adjuvant raw materials, has a ratio of: Tween-80 2.7%, Span-80 2.7%, and the balance is physiological saline.

[0052] The preparation method of the above-mentioned multiple nanoemulsion vaccine adjuvant comprises the following steps;

[0053] 1) Dissolving the immune enhancer in physiological saline, mixing, and ster...

Embodiment 3

[0060] A multiple nanoemulsion vaccine adjuvant consisting of an inner water phase, an intermediate oil phase and an outer water phase;

[0061] Each component in the internal water phase, based on the total weight of the multiple nanoemulsion vaccine adjuvant raw materials, the proportion is: 50.0mg / mL lentinan injection 1.0%;

[0062] Each component in the middle oil phase, based on the total weight of the multiple nanoemulsion vaccine adjuvant raw materials, has a ratio of: 1.0% lecithin, 1.0% ethanol, 3.0% squalene, and 3.0% palm oil;

[0063] Each component in the external water phase, based on the total weight of the multiple nanoemulsion vaccine adjuvant raw materials, has a ratio of: Tween-80 3.2%, Span-80 3.2%, and the balance is physiological saline.

[0064] The preparation method of the above-mentioned water-in-oil-in-water vaccine adjuvant comprises the following steps;

[0065] 1) Fully mix the immune enhancer solution and the inactivated antigen to obtain the i...

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Abstract

The invention discloses a preparation method of a multiple nanoemulsion vaccine adjuvant, which solves the problems of difficult injection and low immunity of the existing vaccine adjuvant. The technical scheme includes firstly dissolving the immune enhancer in physiological saline to obtain an internal water phase; then dissolving the emulsifier and co-surfactant in non-mineral oil and mixing to obtain an intermediate oil phase; then slowly adding the internal water phase to the In the middle oil phase, self-emulsify at a speed of 600-1200rpm to obtain stable water-in-oil colostrum; then add hydrophilic surfactant and lipophilic surfactant to physiological saline, and mix thoroughly with magnetic stirring to obtain external water phase; finally, water-in-oil colostrum is added to the external water phase for emulsification to obtain multiple nanoemulsion vaccine adjuvants. The method of the invention has good emulsion stability and low viscosity (<90mPa·s), and the vaccine adjuvant has reasonable controlled release, targeting and isolation characteristics.

Description

technical field [0001] The invention relates to a preparation method of a vaccine adjuvant, in particular to a preparation method of a multiple nanoemulsion vaccine adjuvant. Background technique [0002] Vaccine adjuvant refers to a substance that can non-specifically change or enhance the body's specific immune response to an antigen, as well as enhance the immunogenicity of the corresponding antigen or change the type of immune response, but has no antigenicity itself. Adjuvants can induce the body to produce a long-term and efficient specific immune response, improve the body's protective ability, and at the same time reduce the dosage of immune substances and reduce the production cost of vaccines. [0003] Usually vaccine adjuvants are divided into three categories according to the dosage form, namely oil-in-water (O / W) type, water-in-oil (W / O) type and water-in-oil-in-water (W / O / W) type (also known as multiple lotion). At present, the W / O / W vaccine adjuvant recogniz...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61K9/113A61K31/715A61P37/04
CPCA61K39/39A61P37/04A61K9/113A61K31/715A61K2039/552A61K2039/55566
Inventor 王春维陶冶程铭焦文杰夏廉臣王雪晖祝爱侠
Owner WUHAN POLYTECHNIC UNIVERSITY
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