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Peptides and their applications

A kind of use and product technology, applied in the direction of peptides, decapeptides, peptide/protein components, etc., can solve the problems of identifying peptide sequences, etc., and achieve the effects of enhancing learning and memory, good application prospects, and alleviating abnormal death

Active Publication Date: 2021-04-13
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this technical solution did not identify a specific peptide sequence from the mixture and should be further investigated

Method used

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  • Peptides and their applications
  • Peptides and their applications
  • Peptides and their applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1. Relief effect of polypeptide on β-amyloid-induced SH-SY5Y cell death

[0025] In this embodiment, SEQ ID NO:1 and SEQ ID NO:7 are respectively used as the polypeptides to be tested.

[0026] Experimental group: SH-SY5Y cells in the logarithmic growth phase were selected, and 3×10 4 cells / mL, inoculated in 96-well plate (100 μL per well), at 37°C, 5% CO 2 Cultivate in the cell incubator for 24 hours (refer to hours, the same below). Add 0.5μM Aβ after 24h of culture 25-35 (that is, β-amyloid protein), and 0.1 mg / mL of the polypeptide to be tested was added, and 6 duplicate wells were set up, and an equal amount of culture medium containing 1% fetal bovine serum was added to the control wells. After culturing for 48 hours, add 10% of the final volume of the medium to each well of MTT solution, incubate in a 37°C constant temperature cell culture incubator for 4 hours, discard the supernatant, add 150 μL of DMSO to each well, and place on a shaker. Shake for...

Embodiment 2

[0030] Example 2. Alleviating effect of polypeptide on β-amyloid-induced death of primary hippocampal neurons

[0031] In this embodiment, SEQ ID NO:1 and SEQ ID NO:7 are respectively used as the polypeptides to be tested.

[0032] Experimental group: DMEM (high sugar) + 10% FBS was used as the planting medium, and Neurobasal + 2% B27 + 0.5mmol / L glutamine was used as the maintenance medium. Coating 96-well plate: add 0.1mL 50μg / mL PDL solution (i.e. poly-lysine solution) to each well, suck it out after 3 hours, put it in the cell incubator to dry overnight; wash the cell plate with sterile PBS solution the next day 3 Then, put them in the cell culture incubator to dry. Rats at 18 days of pregnancy were selected, anesthetized with 10% chloral hydrate solution, and sterilized with 75% alcohol for the whole body; the rats were placed on ice packs, and the fetuses were taken by laparotomy, and placed in sterile pre-cooled PBS solution; Scalp and skull of fecundized rats, starti...

Embodiment 3

[0035] Embodiment 3, the enhancement effect of polypeptide on the learning and memory ability of rats

[0036] In this embodiment, SEQ ID NO:1 and SEQ ID NO:7 are respectively used as the polypeptides to be tested.

[0037] Take 50 male Wistar rats and raise them in an SPF-grade animal room with a temperature of 25±2°C, free access to food and water, and light for 12 hours a day. These experimental rats were randomly divided into 5 groups, 10 in each group, respectively: PBS control group, SEQ ID NO:1 group (2mg / kg), SEQ ID NO:7 group (2mg / kg). The polypeptide to be tested was subcutaneously administered, and the control group was subcutaneously injected with PBS; the administration continued for 14 days. The Morris water maze system was used to evaluate the spatial learning and memory abilities of experimental rats.

[0038] The Morris water maze system mainly includes: a cylindrical pool and a movable platform (10cm in diameter), a video recording system, and a data analys...

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Abstract

The present invention relates to polypeptides and applications thereof. The polypeptide is a series of polypeptides, the sequence is X-GAGSGAG-Y, wherein X and Y are each a defined group or amino acid sequence. The series of polypeptides of the present invention can enhance learning and memory ability, can treat or prevent neurodegenerative diseases (such as Alzheimer's disease), and can relieve abnormal death of nerve cells, and have good application prospects.

Description

technical field [0001] The invention relates to a polypeptide and its application. The polypeptide is related to silk fibroin in silk. The application mainly involves enhancing learning and memory ability and preventing and treating neurodegenerative diseases, and belongs to the technical field of biomedicine. Background technique [0002] Bioactive peptides are peptide compounds that are beneficial to the life activities of the body or have physiological effects, and are a class of multifunctional compounds derived from proteins. Compared with proteins, bioactive peptides have a smaller molecular weight and are easier to be absorbed and utilized. It is one of the hot spots in the fields of biomedicine, health care products and food science research. [0003] Silk is one of the products of ancient Han civilization and one of the earliest animal fibers used by humans. It is rich in 18 kinds of amino acids needed by the human body and is known as the "Fiber Queen". Silk is ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/00A61K38/16A61P25/00A61P25/28A23L33/18
CPCA61K38/00A23L33/18A61P25/00A61P25/28C07K14/00
Inventor 高向东陈松徐政姚思远
Owner CHINA PHARM UNIV
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