Selenium-enriched mushroom strain, and selenium-enriched mushroom and cultivation method thereof
A cultivation method and a strain culture medium technology, which are applied in the field of modern agricultural cultivation, can solve problems such as the low content of organic selenium, which cannot meet the needs of food and medicine, are prone to toxic and side effects, and are harmful to human health, so as to ensure human health and reduce Inorganic selenium, the effect of increasing the content of organic selenium
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0031] According to the following method, the primary bacterial culture medium was prepared:
[0032] (1) Peel the potatoes, remove the bud eyes, weigh 200g, cut into 1-2g hob pieces, put them in a pot, add 1000ml of water, boil and then cook for 10min. Use 4-6 layers of gauze to filter the discarded residue, pour the filtrate back into the pot, add 20g of agar, stir with a glass rod while heating with a slow fire, and add 20g of glucose, 1.5g of potassium dihydrogen phosphate, and 1.5g of dipotassium hydrogen phosphate after the agar is dissolved , magnesium sulfate 3g, vitamin B110mg. Add water to make up to 1000ml, and pour it into a graduated cylinder while it is hot to obtain a liquid medium.
[0033] (2) Place the funnel on the clamp of the iron stand, connect the latex tube at the bottom of the funnel, clamp a spring clip on the tube as a control switch, and connect the glass tube with a pointed mouth under the tube. Pour the liquid medium into the funnel while it is ...
Embodiment 2
[0037] Prepare selenium-enriched oyster mushrooms according to the following steps:
[0038] Step 1. Domestication and screening to obtain first-class strains:
[0039] (1) Select two Pleurotus ostreatus strains with strong stress resistance, fast growth and high yield as candidate Pleurotus ostreatus strains.
[0040] (2) Make the first-grade strain culture medium; The first-grade strain culture medium is PDA medium, and comprises the raw material component of following weight ratio in every 1000mlPDA culture medium: peeled potato 200g, glucose 20g, potassium dihydrogen phosphate 1.5g , dipotassium hydrogen phosphate 1.5g, magnesium sulfate 3g, vitamin B110mg, agar 20g, and the rest is water.
[0041](3) Prepare 18 test tubes, and each group of 9 test tubes is divided into two groups, i.e. the first group and the second group, respectively in the first group and the second group of test tubes, subpackage 8ml of primary bacterial culture medium, and Add 30ppm, 60ppm, 90ppm, ...
Embodiment 3
[0054] Prepare selenium-enriched oyster mushrooms according to the following steps:
[0055] Step 1. Domestication and screening to obtain first-class strains:
[0056] (1) Select five Pleurotus ostreatus strains with strong stress resistance, fast growth and high yield as candidate Pleurotus ostreatus strains.
[0057] (2) Make the first-grade strain culture medium; The first-grade strain culture medium is PDA medium, and comprises the raw material component of following weight ratio in every 1000mlPDA culture medium: peeled potato 200g, glucose 20g, potassium dihydrogen phosphate 1.5g , dipotassium hydrogen phosphate 1.5g, magnesium sulfate 3g, vitamin B110mg, agar 20g, and the rest is water.
[0058] (3) Prepare 45 test tubes, divide each group into five groups according to 9 test tubes, that is, the first group, the second group, the third group, the fourth group and the fifth group. The test tubes of the third group, the fourth group and the fifth group were filled with...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More