Method for deep eutectic solvent extraction and macroporous resin separation and purification of burdock leaf total flavonoids

A low eutectic solvent, separation and purification technology, applied in the field of low eutectic solvent extraction, macroporous adsorption resin separation and purification of burdock leaf total flavonoids, can solve the problems of low extraction rate, environmental pollution, lack of systemicity, etc., and achieves simple operation. , the effect of low cost

Active Publication Date: 2018-10-09
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most of the existing methods for extracting and separating flavonoids from burdock leaves are not systematic, and most of the extraction solvents are traditional solvents such as methanol and ethanol, which not only have a low extraction rate but also cause pollution to the environment

Method used

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  • Method for deep eutectic solvent extraction and macroporous resin separation and purification of burdock leaf total flavonoids
  • Method for deep eutectic solvent extraction and macroporous resin separation and purification of burdock leaf total flavonoids
  • Method for deep eutectic solvent extraction and macroporous resin separation and purification of burdock leaf total flavonoids

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Experimental program
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Effect test

Embodiment 1

[0019] (1) Preparation of deep eutectic solvent: Choline chloride and ethylene glycol were mixed in a molar ratio of 1:2 in a reactor with a drying tube, heated to 80°C, and stirred for 2 hours until a transparent and uniform solvent was formed.

[0020] (2) Preparation of extraction solvent: do not add deionized water to the deep eutectic solvent prepared in step (1), and prepare an extraction solvent with a water content of the deep eutectic solvent of 0%.

[0021] (3) Extraction: the burdock leaves are washed, dried, and pulverized, and the burdock leaf powder and the extraction solvent prepared in step (2) are placed in a reaction vessel at a solid-to-liquid ratio of 10:1 mg / mL. Put the above materials in an ultrasonic-assisted extractor, and conduct an ultrasonic-assisted extraction for 20 minutes at 35°C and an ultrasonic power of 150W to obtain an extract, and filter the extract to obtain the crude extract of burdock leaves, total flavonoids of burdock leaves The extrac...

Embodiment 2

[0024] (1) Preparation of deep eutectic solvent: Choline chloride and ethylene glycol were mixed in a molar ratio of 1:2 in a reactor with a drying tube, heated to 80°C, and stirred for 2 hours until a transparent and uniform solvent was formed.

[0025] (2) Preparation of the extraction solvent: add an appropriate amount of deionized water to the deep eutectic solvent prepared in step (1) to prepare an extraction solvent with a water content of the deep eutectic solvent of 50%.

[0026] (3) Extraction: the burdock leaves are washed, dried, and pulverized, and the burdock leaf powder and the extraction solvent prepared in step (2) are placed in a reaction vessel at a solid-to-liquid ratio of 20:1 mg / mL. Put the above materials in an ultrasonic-assisted extractor, and conduct an ultrasonic-assisted extraction for 40 minutes at 65°C and an ultrasonic power of 300W to obtain an extract, and filter the extract to obtain the crude extract of burdock leaves, total flavonoids of burdo...

Embodiment 3

[0029] (1) Preparation of deep eutectic solvent: Choline chloride and ethylene glycol were mixed in a molar ratio of 1:2 in a reactor with a drying tube, heated to 80°C, and stirred for 2 hours until a transparent and uniform solvent was formed.

[0030] (2) Preparation of the extraction solvent: add an appropriate amount of deionized water to the deep eutectic solvent prepared in step (1) to prepare an extraction solvent with a water content of the deep eutectic solvent of 30%.

[0031] (3) Extraction: the burdock leaves are washed, dried, and pulverized, and the burdock leaf powder and the extraction solvent prepared in step (2) are placed in a reaction vessel at a solid-to-liquid ratio of 40:1 mg / mL. Put the above materials in an ultrasonic-assisted extractor, and conduct an ultrasonic-assisted extraction for 50 minutes at 80°C and an ultrasonic power of 350W to obtain an extract, which is subjected to suction filtration to obtain the crude extract of burdock leaves, total f...

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Abstract

The invention belongs to the technical field of biological extraction and particularly relates to a method for deep eutectic solvent extraction and macroporous resin separation and purification of burdock leaf total flavonoids. The extraction solvent in the method consists of the deep eutectic solvent with water content of 0 to 50 percent, wherein the deep eutectic solvent is prepared by reactingcholine chloride with one of ethylene glycol, glycerol, triethanolamine, levulinic acid and malonic acid. The deep eutectic solvent has unique physicochemical properties of greenness, no pollution, low vapor pressure, no toxicity, biodegradability, good solubility and the like; in addition, the deep eutectic solvent has a good extraction effect on flavonoid compounds. According to the method disclosed by the invention, by adopting the method for ultrasonic assisted green novel deep eutectic solvent extraction and the macroporous resin separation and purification of the burdock leaf total flavonoids, the deep eutectic solvent extracting solution can be directly used for separation and purification of macroporous resin. The method has the characteristics of simple operation, low cost, greenness and environment friendliness, and provides a novel idea for extraction, separation and enrichment of burdock leaves.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a method for extracting with a deep eutectic solvent and separating and purifying total flavonoids from burdock leaves with a macroporous adsorption resin. , good solubility and other unique physical and chemical properties, and has a good extraction effect on flavonoids. Background technique [0002] Burdock (Arctium lappa L.), also known as Dalizi ("Compendium of Materia Medica"), white muscle ginseng, also known as "Oriental radish", "black radish", also known as "Evil real", is a large biennial herb with tap roots of the genus Arctium in the family Asteraceae plant. Burdock is native to my country, mainly wild, and is a traditional medicinal and edible plant in my country. As early as the Southern and Northern Dynasties, it was recorded in the "Famous Doctors", saying that it "long-term service, light body and durable", in the Ming Dynasty Li Shizh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61K8/9789A23L33/105A61K127/00
CPCA23L33/105A23V2002/00A61K8/9789A61K36/28A61K2236/333A61K2236/55A23V2250/2116
Inventor 刘言娟况鹏群张波李海刚李新鹏
Owner LINYI UNIVERSITY
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