Application of golden camellia seed extractive to alpha-glucosidase inhibitor

A technology of glucosidase and golden camellia, which is applied in the directions of drug combination, plant raw materials, medical preparations containing active ingredients, etc., and achieves the effects of mild conditions, broad application prospects and easy operation.

Inactive Publication Date: 2018-10-09
DALIAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the studies on Camellia japonica focus on the leaves, flowers and other parts, while the research on the seeds of Camellia japonica is less, and the effect of inhibiting α-glucosidase on the seeds of Camellia japonica has not been reported yet.

Method used

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  • Application of golden camellia seed extractive to alpha-glucosidase inhibitor
  • Application of golden camellia seed extractive to alpha-glucosidase inhibitor
  • Application of golden camellia seed extractive to alpha-glucosidase inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The preparation of the ethyl acetate part, n-butanol part, water-soluble part of embodiment 1 golden camellia seed ethanol extract and ethanol extract

[0042] 1) Take 800g of dried Camellia japonica seeds and pulverize them with a pulverizer;

[0043] 2) After degreasing petroleum ether, heat and reflux extraction with 3 times the amount of 95% ethanol for 3 times, each time for 3 hours, to obtain Camellia japonica extract;

[0044] 3) The Camellia japonica extract was filtered with filter paper, the filtrates were combined, concentrated under reduced pressure to obtain an extract, and 76 g of the ethanol extract of Camellia japonica seeds was obtained; after taking 71 g of the ethanol extract of Camellia japonica seeds and adding an appropriate amount of water to suspend, successively distilled with ethyl acetate , n-butanol extraction, to obtain ethyl acetate part, n-butanol part and raffinate aqueous layer part, after concentrating, vacuum-dry to obtain ethyl acetat...

Embodiment 2

[0045] Example 2 Evaluation of inhibitory effect on α-glucosidase activity

[0046] Using percentage inhibition rate (I%) and half inhibitory concentration (IC 50 Value) combined method for evaluation. IC 50 Calculated using GraphPad Prism software, the percentage inhibition rate was calculated as follows:

[0047]

[0048] 1. Determination of α-glucosidase inhibitory activity of the ethanol extract of Camellia japonica seeds

[0049] The ethanol extract of Camellia japonica seeds was prepared with DMSO into sample solutions with five different concentrations of 0.2, 0.5, 1, 1.5, and 2 mg / ml, corresponding to final concentrations of 10, 25, 50, 75, and 100 μg / ml. Set up four groups, namely negative control group, blank group, sample measurement group, and sample control group; add 112 μl PBS, 20 μl 0.2U / ml α-glucosidase, 8 μL DMSO to the negative control group in turn, and incubate at 37°C for 15 minutes in a constant temperature incubator , add 20μl 2.5mmol / l PNPG, inc...

Embodiment 3

[0065] Example 3 Inhibition Types of α-Glucosidase Inhibition

[0066] According to the Lineweave-Burk mapping method, with 1 / [S] as the abscissa and 1 / V as the ordinate, draw the inhibition kinetic curves of the samples respectively, determine the inhibition type of the sample according to the position of the intersection point of the straight lines, and determine the inhibition type of the sample according to the position of the straight line intersection, according to the straight line when no sample is added Intersection with the x-axis = -1 / K m Get the K of α-glucosidase m value, and then according to the non-competitive inhibition kinetic equation: 1 / V′ max =1 / V max (1+[I] / K i ) and competitive inhibition kinetic equation: 1 / K m '=1 / {K m (1+[I] / K i )}, respectively calculate the K of the sample i value.

[0067] 1. Types of inhibition of α-glucosidase inhibitory effect of the ethanol extract of Camellia japonica seeds

[0068] The ethanol extract of camellia jap...

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Abstract

The invention discloses application of golden camellia seed extractive to alpha-glucosidase inhibitor, in particular to a health care product and a medicine applied to the prevention and the curing ofthe type 2 diabetes mellitus. An in vitro alpha-glucosidase activity model is adopted for investigating the inhibition function of golden camellia seed alcohol extracts and the ethyl acetate part, the n-butyl alcohol part and the aqueous layer part of the alcohol extract for the alpha-glucosidase, and an inhibition type is determined through a Lineweave-Burk plotting method. The preparation method for a target product is simple, is easy in operation, has a potential of industrial production and can be used for the development application of the alpha-glucosidase inhibitor in natural products,and an inhibition rate for the alpha-glucosidase is superior to that of clinic medicine acarbose.

Description

technical field [0001] The invention relates to the field of medicine and health care products, in particular to the application of camellia camellia seed extract in alpha-glucosidase inhibitors. Background technique [0002] Type 2 diabetes, also known as non-insulin-dependent diabetes, is a common disorder of glucose and fat metabolism. Diabetes is characterized by high concentrations of blood sugar, which may lead to serious complications in the kidneys, eyes and cardiovascular system. It is chronic Metabolic diseases can cause metabolic disorders such as sugar, protein, and fat, and damage the blood circulation system (Morrison, F., Shubina, M., & Turchin, A. Encounter frequency and serum glu-cose level, bloodpressure, and cholesterol level control in patients with diabetes mellitus [J]. Archives of Internal Medicine. 2001, 171, 1542–50.). Therefore, the treatment of diabetes mainly focuses on reducing blood sugar fluctuations and mitigating complications. Drugs that a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/82A61P3/10A61K131/00
CPCA61K36/82A61K2236/333A61K2236/39A61K2236/51A61P3/10
Inventor 于大永宋昱黎玉梅史丽颖
Owner DALIAN UNIV
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