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A method of ultrafiltration membrane applied to the separation of exopolysaccharides from Cryptodinium algae

A technology for the separation of cryptodinosaur cells and separation methods, which is applied in the field of separation of extracellular polysaccharides of cryptodinosaurs by ultrafiltration membranes, can solve the problems of high separation cost, large amount of organic solvent, and enlarged preparation process, and achieve simple process, Strong applicability, high purity effect

Active Publication Date: 2020-12-29
自然资源部天津海水淡化与综合利用研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the preparation process described in this patent, only ultrafiltration concentration and ethanol precipitation are simply adopted, and the concentration of exopolysaccharide of Cryptodinium algae obtained is low, and the recovery rate and purity are not mentioned, which is not suitable for the enlargement of the preparation process
In the traditional exopolysaccharide preparation process, the fermentation supernatant from the algae body is alcohol-precipitated to obtain crude polysaccharides, and then the protein is removed by the Sevage method to obtain higher purity polysaccharides, but the amount of organic solvent is large, the separation cost is high, and the polysaccharides It is easy to have organic reagent residues in the medium, which is not suitable for rapid mass production

Method used

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  • A method of ultrafiltration membrane applied to the separation of exopolysaccharides from Cryptodinium algae
  • A method of ultrafiltration membrane applied to the separation of exopolysaccharides from Cryptodinium algae
  • A method of ultrafiltration membrane applied to the separation of exopolysaccharides from Cryptodinium algae

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Experimental program
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Effect test

Embodiment 1

[0028] (1) Select the fermentation liquid of the exopolysaccharide of Cryptodinium algae, centrifuge it for 15 minutes at a rotating speed of 5000 rpm, take the supernatant and filter it through a microporous membrane with a pore diameter of 0.22 μm, and obtain the fermentation supernatant for removing the algae body;

[0029] (2) 1 L of the fermentation supernatant was spray-dried by a spray dryer and redissolved in distilled water to obtain a concentrate; the volume of the fermentation supernatant was 10 times the volume of the concentrate.

[0030] The spray drying conditions are: the inlet temperature of the spray dryer is 120°C, the outlet temperature of the spray dryer is 70°C, and the feed rate of the spray dryer is 500mL / h;

[0031] (3) adding absolute ethanol to the concentrated solution, standing and centrifuging to obtain a precipitate; the volume ratio of absolute ethanol to the concentrated solution is 3;

[0032] (4) Redissolve the precipitate in distilled water,...

Embodiment 2

[0037] (1) Select the fermentation liquid of exopolysaccharide of Cryptodinium algae, centrifuge it at 10000rpm for 20 minutes, take the supernatant and filter it through a microporous membrane with a pore diameter of 0.22 μm, and obtain the fermentation supernatant that removes the algae body;

[0038] (2) 1 L of the fermentation supernatant was reconstituted with distilled water after being spray-dried by a spray dryer to obtain a concentrate; the volume of the fermentation supernatant was 15 times the volume of the concentrate;

[0039] The spray drying conditions are: the inlet temperature of the spray dryer is 80°C, the outlet temperature of the spray dryer is 60°C, and the feed rate of the spray dryer is 300mL / h;

[0040] (3) adding absolute ethanol to the concentrated solution, standing and centrifuging to obtain a precipitate; the volume ratio of absolute ethanol to the concentrated solution is 2;

[0041] (4) Redissolve the precipitate in distilled water, then place i...

Embodiment 3

[0046] (1) Select the fermentation liquid of the extracellular polysaccharide of Cryptodinoflagellates, centrifuge at a speed of 8000rpm for 30min, take the supernatant and filter it through a microporous membrane with a pore diameter of 0.22 μm, and obtain the fermentation supernatant that removes the algal bodies;

[0047] (2) 1 L of the fermentation supernatant was reconstituted with distilled water after being spray-dried by a spray dryer to obtain a concentrate; the volume of the fermentation supernatant was 12 times the volume of the concentrate;

[0048] The spray drying conditions are: the inlet temperature of the spray dryer is 140°C, the outlet temperature of the spray dryer is 80°C, and the feed rate of the spray dryer is 1000mL / h;

[0049] (3) adding absolute ethanol to the concentrated solution, standing and centrifuging to obtain a precipitate; the volume ratio of absolute ethanol to the concentrated solution is 4;

[0050] (4) Redissolve the precipitate in disti...

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Abstract

The invention discloses a method for separating crypthecodinium cohnii exopolysaccharides by applying an ultrafiltration membrane. The method comprises the following steps: centrifugally separating afermentation liquid of the crypthecodinium cohnii exopolysaccharides, and filtering by a microfiltration membrane to remove fronds to obtain a fermentation supernatant; spray-drying the fermentation supernatant by a spray dryer and re-dissolving by using distilled water to obtain a concentrated liquid; adding absolute ethanol into the concentrated liquid, standing and centrifuging to obtain precipitate; re-dissolving the precipitate into the distilled water, putting into a dialysis bag and collecting a dialysis tapped fluid; and performing ultrafiltration on the dialysis tapped fluid by an ultrafiltration group device. The process is simple and convenient, the use amount of an organic solvent is small, and the extraction and the separation purity of the crypthecodinium cohnii exopolysaccharides is high. The method has high applicability.

Description

technical field [0001] The invention relates to a membrane separation method, in particular to a method for separating extracellular polysaccharides of Cryptodinium dinoflagellates with an ultrafiltration membrane. Background technique [0002] Biological polysaccharides have the advantages of being non-toxic and harmless to the human body, and have a high safety factor. They are used as food additives such as thickeners and emulsifiers, and are widely used in the field of food processing. Blood coagulation and other biological activities have broad application prospects in medicine. The polysaccharides used in the clinical fields of food and medicine are mainly occupied by plant polysaccharides such as traditional Chinese medicine, large seaweed polysaccharides, and bacterial polysaccharides. Due to the growth conditions and environmental characteristics of marine microalgae, it may have some structures and functions different from those of terrestrial plant polysaccharide...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
CPCC08B37/00C08B37/0003
Inventor 杜瑾张爱君张晓青郝建安曹军瑞王树勋司晓光
Owner 自然资源部天津海水淡化与综合利用研究所
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