Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for refining pumpkin oligosaccharides by using macroporous resin

A technology of macroporous resin and refining method, which is applied in the preparation of oligosaccharides and sugar derivatives, chemical instruments and methods, etc., can solve the problems of low removal rate of impurities, poor decolorization effect of colored substances, and destruction of oligosaccharide structure, etc. Achieve high purity, less waste, and low energy consumption

Active Publication Date: 2018-10-12
国农绿源(北京)农业科技研究院有限公司
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for refining pumpkin oligosaccharides by using macroporous resin, so as to overcome the shortcomings of poor decolorization effect of colored substances in existing pumpkin oligosaccharides, low removal rate of impurities, and destruction of oligosaccharide structure

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for refining pumpkin oligosaccharides by using macroporous resin
  • Method for refining pumpkin oligosaccharides by using macroporous resin
  • Method for refining pumpkin oligosaccharides by using macroporous resin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A method for refining pumpkin oligosaccharides by using macroporous resin, carried out according to the following steps:

[0050] (1) Macroporous resin pretreatment: Soak DM28 macroporous adsorption resin in deionized water for 24 hours, wash with ethanol until it is not cloudy, soak in ethanol for 24 hours, wash with water until there is no alcohol smell, and use a NaOH solution with a mass percentage concentration of 5%. Pass through the resin column and soak for 2h, wash with water until neutral, pass HCl with a mass percentage concentration of 5% through the resin column and soak for 4h, wash with water until neutral, and set aside at 4°C; when in use, pack into a chromatography column (6cm×80cm) 3 / 4 places, to ensure that the filling is even and flat without air bubbles;

[0051] (2) Add water to the pumpkin oligosaccharide crude extract to form a solution with a concentration of 250 mg / ml, and pass the above-mentioned solution of 1.0 times column volume (BV) thro...

Embodiment 2

[0063] A method for refining pumpkin oligosaccharides by using macroporous resin, carried out according to the following steps:

[0064] (1) Macroporous resin pretreatment: Soak DM21 macroporous adsorption resin in deionized water for 30 hours, wash with ethanol until it is not cloudy, soak in ethanol for 30 hours, wash with water until there is no alcohol smell, and use a NaOH solution with a mass percentage concentration of 5%. Pass through the resin column and soak for 4 hours, wash with water until neutral, pass HCl with a mass percentage concentration of 5% through the resin column and soak for 4 hours, wash with water until neutral, and set aside at 4°C; when used, it is loaded into 3 / 4 of the chromatography column to ensure The filling is even and smooth without air bubbles;

[0065] (2) add water to pumpkin oligosaccharide crude extract to form a solution with a concentration of 280 mg / ml, and pass the above-mentioned solution of 1.5 times column volume (BV) through a ...

Embodiment 3

[0075] The pumpkin oligosaccharide crude extract used in this example is the pumpkin oligosaccharide crude extract prepared in Example 1.

[0076] A method for refining pumpkin oligosaccharides by using macroporous resin, carried out according to the following steps:

[0077] (1) Macroporous resin pretreatment: soak DM28 macroporous adsorption resin in deionized water for 20 hours, wash with ethanol until it is not cloudy, soak in ethanol for 20 hours, wash with water until there is no alcohol smell, and use a NaOH solution with a mass percentage concentration of 5%. Pass through the resin column and soak for 1h, wash with water until neutral, pass HCl with a mass percentage concentration of 5% through the resin column and soak for 1h, wash with water until neutral, and set aside at 4°C; when in use, pack into a chromatography column (6cm×80cm) 3 / 4 places, to ensure that the filling is even and flat without air bubbles;

[0078] (2) Add water to the pumpkin oligosaccharide c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for refining pumpkin oligosaccharides by using macroporous resin. The method comprises the following steps: adding water to a pumpkin oligosaccharide crude extract toprepare a pumpkin oligosaccharide column-loading solution, performing absorption by chromatography columns loaded with macroporous resin and collecting effluent liquid; performing standing, rinsing the macroporous resin with deionized water, and performing concentration by evaporation and freeze-drying to obtain colorless pumpkin oligosaccharides; performing washing with an ethanol solution and soaking with ethanol, allowing an NaOH solution to pass through and soak the resin columns, and performing water washing to neutral; allowing hydrochloric acid to pass through and soak the resin columns, and recovering the macroporous resin; and performing rotary evaporation and freeze drying, and recovering colored impurities and the ethanol. By the refining method, high-efficiency discoloration ofthe pumpkin oligosaccharides is achieved while monosaccharides and protein impurities are simultaneously removed, the purity of the obtained pumpkin oligosaccharides is as high as 95-96%, also the process has mild treatment conditions, does not adopt high-temperature or acid and alkali treatment and cannot cause degradation of the pumpkin oligosaccharides, and the obtained colored impurity melanoidin can be recovered and utilized.

Description

technical field [0001] The invention belongs to the technical field of purifying pumpkin oligosaccharides, and in particular relates to a method for refining pumpkin oligosaccharides by using a macroporous resin. Background technique [0002] A large number of studies have confirmed that functional sugar components such as polysaccharides and oligosaccharides in pumpkin have blood sugar regulating functions, and this functional sugar is a natural plant-derived component, and its safety is also widely recognized. Oligosaccharides (oligosaccharides) can also be called oligosaccharides, which are low-degree polymerized sugars connected by 2-10 monosaccharides through glycosidic bonds to form straight or branched chains, with a molecular weight between 200 and 3000D. Due to the different binding positions and types of monosaccharide molecules, a rich variety of oligosaccharides is formed. Oligosaccharides have good functional properties and can be used as a source of sweetness,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07H1/06C07H3/04C07H3/06C08B37/00C09B61/00A23L5/43A23L33/10A61P39/06A61P3/10A61P1/02
CPCA23V2002/00A23L5/43A23L33/10A61P1/02A61P3/10A61P39/06C07H1/06C07H3/04C07H3/06C08B37/003C09B61/00A23V2200/02A23V2200/04A23V2200/312A23V2200/328
Inventor 李全宏梁莉
Owner 国农绿源(北京)农业科技研究院有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com