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Aquaculture water body water purifying agent and preparation method thereof

A technology for aquaculture water and water purifiers, applied in biochemical equipment and methods, chemical instruments and methods, biological water/sewage treatment, etc., can solve problems such as unstable effects, increased application costs, and difficulty in obtaining, and achieve treatment effects Stabilization, maintenance of ecological balance, and the effect of strain acquisition

Inactive Publication Date: 2018-10-12
北京天诚众合科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some defects in the current microbial preparations: large dosage, unstable effect, unclear source of strains (such as commercially available), limited use conditions, and cannot satisfy all aquaculture water bodies.
However, the above-mentioned combination has not yet achieved the best water purification effect in treating ornamental fish aquaculture water, and the composition has complex bacterial species, especially Bacillus coagulans is difficult to obtain, which increases the application cost

Method used

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  • Aquaculture water body water purifying agent and preparation method thereof
  • Aquaculture water body water purifying agent and preparation method thereof

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preparation example Construction

[0035] Invent the preparation method of above-mentioned aquaculture water purifying agent, comprise the steps:

[0036] S1. Screen the pond water body in the late stage of intensive culture, collect 1.0g of water sample, put it into a 50mL triangular flask filled with 9mL sterile water and glass beads, shake it on a shaker for 20min to disperse the microbial cells, and let it stand for 30s. into 10 -1 Diluent;

[0037] S2. Use a 1mL sterile pipette to draw 10 -1 Dilute 1mL, transfer to a test tube filled with 9mL sterile water, suck and blow 3 times, mix the bacterial solution, and serve as 10 -2 Diluent; and so on, serially diluted to make 10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 A series of diluted bacterial solutions;

[0038] S3, from 10 -6 、10 -7 、10 -8 Take 1mL of each of the diluted bacterial solutions on the plate, pour into the medium that has been melted and cooled to 45-50°C, turn the plate gently to mix the bacterial liquid with the medium evenly; after...

Embodiment 1

[0042] A water purifying agent for aquaculture, comprising the following components with the ratio of bacteria numbers: Bacillus megaterium, Bacillus cereus, Thiobacillus denitrificans, Lactobacillus acidophilus, Nitrobacter victorii, the number ratio of bacteria is 2: 2:1:2:1, the addition amount is 5ppm.

[0043] The preparation method of the above-mentioned aquaculture water purifying agent is as follows:

[0044] S1. Screen the pond water body in the late stage of intensive culture, collect 1.0g of water sample, put it into a 50mL triangular flask filled with 9mL sterile water and glass beads, shake it on a shaker for 20min to disperse the microbial cells, and let it stand for 30s. into 10 -1 Diluent;

[0045] S2. Use a 1mL sterile pipette to draw 10 -1 Dilute 1mL, transfer to a test tube filled with 9mL sterile water, suck and blow 3 times, mix the bacterial solution, and serve as 10 -2 Diluent; and so on, serially diluted to make 10 -3 、10 -4 、10 -5 、10 -6 、10 -...

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Abstract

The invention discloses an aquaculture water body water purifying agent and a preparation method thereof. The water purifying agent is composed of bacillus megaterium, bacillus cereus, thiobacillus denitrificans, lactobacillus acidophilus and nitrobacter winogradsky, the quantity ratio of the bacteria number is (1.8-2.2):(1.8-2.2):(0.8-1):(1.8-2.2):(0.8-1), and the total number of bacteria reaches10billion / g. The aquaculture water body water purifying agent screens the bacteria in a targeted mode, and is prepared by combining the bacillus megaterium, the bacillus cereus, the thiobacillus denitrificans, the lactobacillus acidophilus and the nitrobacter winogradsky which are compounded together to exert the synergistic effect and function of microorganisms, degradation and conversion are performed on organic matters, ammonia nitrogen, nitrite and other pollutants in a water body, and the purposes of purifying water quality and repairing the water body are achieved. The aquaculture waterbody water purifying agent can convert complex organic matters into simple inorganic substances required for growth and reproduction of plankton and maintain the ecological balance of the aquacultureenvironment without any toxic or side effects and with low costs.

Description

technical field [0001] The invention belongs to the technical field of water treatment, in particular to a water purifying agent for aquaculture water and a preparation method thereof Background technique [0002] Aquaculture water pollution refers to the phenomenon that the water body is destroyed and the balance of water quality factors is out of balance due to factors such as the secretions and excreta of the breeding objects, excess bait, drug residues, and human activities (production, life). In recent years, my country's aquaculture industry has developed rapidly, and the scale of aquaculture has continued to expand. However, at present, most of the aquaculture in our country adopts a high-density intensive farming model. The seedlings are overstocked, and a large amount of fertilization and baiting are used to make residual bait, aquatic organism excrement and corpses, sediments at the bottom of aquaculture water, and various organic and inorganic fertilizers. Such e...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/11C12R1/085C12R1/23C02F103/20
CPCC02F3/341C02F3/348C02F2103/20C12N1/20
Inventor 刘立明李海兵韦嵩王胜男
Owner 北京天诚众合科技发展有限公司