A methylotrophic Bacillus and its application

A methylotrophic, bacillus technology, applied in applications, bacteria, fungicides, etc., can solve problems such as few disease-resistant varieties, poor chemical pesticide control effects, and environmental pollution

Active Publication Date: 2021-06-15
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems that there are few disease-resistant varieties of corn stalk rot, the control effect of chemical pesticides is poor, and the environment is polluted, the purpose of the present invention is to provide a methylotrophic bacillus

Method used

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  • A methylotrophic Bacillus and its application
  • A methylotrophic Bacillus and its application
  • A methylotrophic Bacillus and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Isolation of methylotrophic bacillus TA-1

[0028] The maize rhizosphere soil collected from Tai’an City, Shandong Province in September 2014 was stored at 4 °C for isolation of antagonistic bacteria.

[0029] Weigh 10 g of sieved soil sample, put it into a 250 mL sterilized Erlenmeyer flask filled with 90 mL of sterile water and a small amount of glass beads, place the Erlenmeyer flask on a shaker at 37 °C and 200 r / min for 20 min, and make Suspend it sufficiently, then treat it in a water bath at 80°C for 10 min, and dilute the bacterial suspension to 10 with sterile water. -3 、10 -4 、10 -5 、10 -6 isogradient. Draw 100 µL of each gradient solution and evenly spread it on the LB medium plate, repeat each gradient 3 times, place in a constant temperature incubator at 30 °C for 1-2 days, pick different single colonies and purify by streaking on the plate. Store at 4°C until testing. Fusarium graminearum ( F. graminearum ), Rhizoctonia solani ( Rhizoct...

Embodiment 2

[0034] Example 2 Identification of Methylotrophic Bacillus TA-1

[0035] 2.1 Colony morphology and physiological and biochemical properties of strain TA-1

[0036] The activated strain TA-1 was streaked and inoculated on the NA plate, cultured at 37 °C for 24 h, and the growth, color and morphological characteristics of the colony were observed: the microscopic morphology of the strain TA-1 was rod-shaped, capable of producing spores, with Capsule. Normal growth on the NA plate, the single colony is irregularly round, with bulges on the edge, dry and wrinkled surface, opaque, and the colony is milky white ( figure 1 ); Bacteria formed when the LB liquid medium was static.

[0037] The indicators for the determination of physiological and biochemical properties mainly include Gram staining, VP (Voges-Proskauer) test, contact enzyme reaction, indole production reaction, salt tolerance, starch hydrolysis activity, casein hydrolysis test, citrate utilization test, methyl Red (...

Embodiment 3

[0051] Example 3 Pot control effect of methylotrophic bacillus TA-1 fermentation broth

[0052] Fusarium graminearum was inoculated into a 250 mL sterilized Erlenmeyer flask filled with 100 mL of CMC medium, and cultured on a shaker at 25 °C and 200 r / min for 3-5 days to obtain macroconidia. Remove the mycelium by filtering with sterile filter paper, collect the conidia suspension, and adjust its concentration to 10 with a hemocytometer. 5 Spores / mL. The acquisition of TA-1 fermented liquid is as embodiment 2, and gradient dilution becomes each concentration (10 8 -10 5 CFU / mL).

[0053] Firstly, corn seeds with full grains and the same size are selected, and sterilized with 1% NaClO. Then the corn seeds were soaked in different concentrations of TA-1 fermentation broth (10 4 -10 8 CFU / mL) for 2 h, air-dried overnight and sowed in nutrient pots (diameter 15×height 12 cm) filled with sterilized natural soil and seedling substrate, sowed 8 corn seeds in each pot, and soa...

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Abstract

The invention provides a strain of methylotrophic Bacillus TA-1, the preservation number of which is CCTCC No.M 2018362. The above-mentioned methylotrophic bacillus or / and fermentation product can be used for preventing and treating corn stalk rot, tomato cinerea, rice sheath blight, capsicum phytophthora, cotton fusarium wilt and tobacco black shank; growth; it has the application prospect of preparing microbial preparations and fertilizers.

Description

technical field [0001] The invention belongs to the field of microbial application technology and biological control, and in particular relates to a methylotrophic bacillus and its application in preventing and controlling agricultural diseases. Background technique [0002] Maize is an important food crop in my country. Maize stalk rot is a serious and widely spread soil-borne disease on maize, which poses a great threat to the yield and quality of maize and is widely distributed all over the world. The occurrence of maize stalk rot was first detected in my country in the 1920s, and has rapidly expanded since then. Currently, it has occurred in Henan, Shandong, Xinjiang, Guangxi and other places, and the disease prevalence is showing a trend of intensification. The onset of corn stalk rot is sudden and explosive. The field incidence rate can be maintained at about 15% under normal conditions, and can reach more than 80% when the disease occurs seriously. Although stalk rot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00C05F11/08C05G3/60C12R1/07
CPCA01N63/00A01N63/10C05F11/08C05G3/00C12N1/20C12N1/205C12R2001/07
Inventor 乔康程星凯姬小雪张典利王红艳
Owner SHANDONG AGRICULTURAL UNIVERSITY
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