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Sisal pgip gene and its application

A gene and sisal technology, applied in the field of plant molecular biology, can solve the problems of damaged sisal industry, lack of resistance to zebra stripe disease, unclear pathogenic mechanism and disease resistance mechanism of zebra stripe disease, and improve resistance Effect

Active Publication Date: 2021-10-22
SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Zebra stripe disease is one of the main diseases of sisal. Due to the occurrence of zebra stripe disease, the sisal industry has been severely damaged. Although a set of prevention and control aspects of zebra stripe disease has been formed in production, the current pathogenicity of zebra stripe disease The mechanism and disease resistance mechanism are still unclear, and there is no new variety resistant to zebra stripe disease in the short term to replace

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  • Sisal pgip gene and its application
  • Sisal pgip gene and its application
  • Sisal pgip gene and its application

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Experimental program
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Embodiment 1

[0018] The two sisal PGIP genes provided by the present invention are all obtained from sisal H.11648, wherein AhPGIP1 The nucleic acid sequence of the gene is SEQ ID No.1, and the amino acid sequence is SEQ ID No.2. AhPGIP2 The nucleic acid sequence of the gene is SEQ ID No.3, and the amino acid sequence is SEQ ID No.4. AhPGIP1 Gene-specific PCR amplification primers are SEQ ID No.5 and SEQ ID No.6. AhPGIP2 Gene-specific PCR amplification primers are SEQ ID No.7 and SEQ ID No.8. AhPGIP1 Gene fluorescent quantitative PCR amplification primers are SEQ ID No.9 and SEQ ID No.10; AhPGIP2 Gene fluorescent quantitative PCR amplification primers are SEQ ID No.11 and SEQ ID No.12; AhPGIP1 The amplification primers with restriction sites are SEQ ID No.13 and SEQ ID No.14. AhPGIP2 The amplification primers with restriction sites are SEQ ID No.15 and SEQ ID No.16. The internal reference gene GAPDH fluorescence quantitative PCR primers are SEQ ID No.17 and SEQ ID No.18.

[0019]...

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Abstract

The present invention adopts the method of PCR to clone 2 sisal PGIP genes, AhPGIP1 and AhPGIP2 ; AhPGIP1 The full length is 1008bp, the protein molecular weight is about 36.7kD, and the isoelectric point is 8.65; AhPGIP2 The full length is 981bp; it is preliminarily confirmed that the PGIP gene can improve the resistance of sisal and tobacco to Phytophthora nicotianae by transient expression, which not only provides genetic resources and theoretical basis for the genetic improvement of sisal, but also provides genetic improvement for other crops related to disease resistance. Genetic resources and theoretical basis.

Description

technical field [0001] The present invention relates to a method of cloning the sisal PGIP gene based on the existing nucleic acid sequence, and using real-time fluorescence quantitative RT-PCR and transient expression methods to study the expression and function of the PGIP gene under different adversity stresses, belonging to plant molecular biology field of technology. Background technique [0002] Polygalacturonase inhibitory proteins (PGIPs) are a class of multifunctional proteins related to plant autoimmunity, which contain 10 incomplete eLRRs, each eLRR consists of 24 residues, and the amino acid sequence is usually LxxLxx Lx Lxx Nx Lt / sgx. Its mechanism of action is to reduce the damage of PGs to the plant cell wall by inhibiting the activity of pathogenic fungi PGs, and at the same time promote the formation of oligogalacturonides through the interaction of PGs and PGIPs, and stimulate a series of defense responses, thereby improving the plant's resistance to patho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12Q1/6895
CPCC07K14/415C12Q1/6895C12Q2600/166
Inventor 张燕梅周文剑王瑞芳杨子平鹿志伟李俊峰陆军迎
Owner SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI