Multi-channel single-protein magnetic tweezer measurement and control method and system

Abstract

The invention discloses a multi-channel single-protein magnetic tweezer measurement and control method and system, the method comprises the steps of: first preparing a protein sample, identifying a plurality of microbeads to be tracked in a field of view by automatic detection or manual intervention, and simultaneously selecting an appropriate reference microbead; then controlling lifting and rotation of a magnet by a motor to control the sizes of stress and torque of the microbeads in the sample, performing real-time display after correction, measuring the three-dimensional coordinates of themulti-channel microbeads by a microbead positioning and tracking technology, displaying in real time the real-time change of the length of a protein fastening the microbeads through the waveform; storing the corrected real-time displayed sizes of the stress and the torque of the microbeads and the real-time displayed length of the protein, after the plurality of microbeads to be tracked in the field of view are completely collected and processed, changing to a next field of view of the sample, and repeating the above steps to measure the mechanical properties of the molecule of the protein. The method can realize simultaneous fast sampling of a plurality of research objects and realize high-throughput measurement.

Description

technical field [0001] The invention relates to the fields of microscopic measurement and control and protein molecular detection, in particular to a multi-channel single protein magnetic tweezers measurement and control method and system. Background technique [0002] Like spectra, each biomolecule has its own unique force spectrum. The force spectrum refers to the relationship between the force on the molecule and the deformation. The study of investigating molecular functions and detecting molecular properties by force spectroscopy is in the ascendant. Mechanics-based operation and detection technologies, such as: optical tweezers, magnetic tweezers, atomic force microscopes, bio-force probes, etc. have been greatly developed, and the measurement accuracy is high. Class. However, in comparison, magnetic tweezers can perform mechanical tensile tests on sample molecules with a wide range of forces without damage to biomolecules, from sub-picotons close to physiological c...

AI Technical Summary

Problems solved by technology

But these techniques, including existing magnetic tweezers, can only observe one molecule at a time, and single-molecule methods are time-consuming to obtain statistically significant results.

[0003] On the other hand, the detection of protein structure and function generally uses X-ray diffraction, electron microscopy and other technologies, which generally can only observe static images, not dynamic observations.

Moreover, these devices are expensive, and for functional tests related to protein molecules, the sample preparation process is complicated, and generally requires genetic engineering recombination to add fluorescent groups, histidine tags and other tags

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