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Scorpion-derived active protein

A technology of active protein and scorpion, which is applied in the direction of non-central analgesics, peptide preparation methods, animal/human peptides, etc., can solve the problems of low extraction rate, unsatisfactory activity and purity of scorpion active protein, etc., and improve pharmacological Effects, reduced side effects, high activity and high purity effects

Inactive Publication Date: 2018-10-16
金华市飞凌生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction method research of scorpion protein mainly includes water decoction, alcohol extraction and enzymatic hydrolysis, etc., the application of enzymatic hydrolysis is the most common, and also has general pharmacological effects, but the activity and purity of scorpion active protein obtained by extraction are not very high. Ideal, with low extraction rates

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Scorpion active protein. The active protein has high activity and purity, and has multiple pharmacological effects such as anti-tumor, analgesic, anti-epileptic, and anti-thrombotic. Pharmacological action, and the active protein is not prone to immunogenicity and allergic reaction to the body, reduces side effects, and has good development prospects.

[0017] The extraction method of scorpion active protein, it specifically comprises the following steps:

[0018] Step 1: Add water to the dried scorpion powder according to the solid-to-liquid ratio of 1:15, then add shikimic acid and p-hydroxycinnamic acid, soak for 25 minutes, wet ultrafine pulverize for 20 minutes, and then centrifuge at 5000r / min for 15 minutes , the supernatant is the extraction solution for later use. The dissolution of the active protein in this step has the advantages of small diffusion resistance, short dissolution path, and large dissolution area, so that the crushing and extraction can be com...

Embodiment 2

[0025] The extraction method of scorpion active protein, it specifically comprises the following steps:

[0026] Step 1: Add water to the dried scorpion powder according to the solid-to-liquid ratio of 1:12, then add shikimic acid and p-hydroxycinnamic acid, soak for 30 minutes, wet ultrafine pulverize for 15 minutes, and then centrifuge at 8000r / min for 10 minutes , the supernatant is the extract for later use;

[0027] Step 2: adjust the pH of the extract obtained in step 1 to 7.0, then ultrafilter through a 5.5KDa molecular weight hollow fiber membrane for 25min under a pressure of 0.3Mpa, rinse the retentate repeatedly with distilled water, concentrate to a relative concentration of 1g / mL, spare;

[0028]Step 3: Add saturated ammonium sulfate solution to the concentrated solution of step 2 until its concentration is 70%, let it stand overnight under low temperature conditions, then centrifuge at 8000r / min for 10min, and use an equivalent concentration of 0.01mol / L, Sodiu...

Embodiment 3

[0033] The extraction method of scorpion active protein, it specifically comprises the following steps:

[0034] Step 1: Add water to the dried scorpion powder according to the solid-to-liquid ratio of 1:10, then add shikimic acid and p-hydroxycinnamic acid, soak for 35 minutes, wet ultrafine pulverize for 10 minutes, and then centrifuge at 10000r / min for 8 minutes , the supernatant is the extract for later use;

[0035] Step 2: adjust the pH of the extract obtained in step 1 to 7.5, then ultrafilter through a 6KDa molecular weight hollow fiber membrane for 20min under a pressure of 0.25Mpa, rinse the retentate repeatedly with distilled water, concentrate to a relative concentration of 1.2g / mL, spare;

[0036] Step 3: Add saturated ammonium sulfate solution to the concentrated solution of step 2 to a concentration of 65%, let stand overnight under low temperature conditions, then centrifuge at 10000r / min for 8min, and use an equivalent concentration of 0.012mol / L, Sodium pho...

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Abstract

The invention discloses scorpion-derived active protein. An extraction method of the active protein comprises the following steps: adding water, shikimic acid and p-hydroxycinnamic acid into dried coarse whole scorpion powder, soaking, performing wet ultramicro pulverization, and then centrifuging to obtain a supernatant as an extract; adjusting the pH of the extract, then performing ultrafiltration, washing a retentate by using water, and concentrating for use; adding a saturated ammonium sulfate solution into a concentrate, standing, centrifuging, thoroughly dissolving a precipitate by usingan equal amount of sodium phosphate buffer, and dialyzing to obtain a dialyzeate as a crude protein solution; sequentially purifying the crude protein solution through filtration by using a cellulosecolumn or a dextran gel, and performing freeze drying to obtain the scorpion-derived active protein. The scorpion-derived active protein has the beneficial effects as follows: the active protein is high in activity and purity; the active protein contains a relatively large variety of proteins achieving a synergist effect, so that the pharmacological action of the active protein is enhanced; in addition, the active protein is not prone to immunogenic and allergic response to a body, so that side effects are reduced; the active protein has a relatively good development prospect.

Description

technical field [0001] The invention relates to the technical field of biological active ingredients, in particular to scorpion active protein. Background technique [0002] Scorpions belong to the Arthropoda phylum of the animal kingdom, the class Arachnida, and the order Scorpion. It is one of the oldest known terrestrial arthropods. At present, there are 6 families, 70 genera, and more than 650 species of scorpions known in the world. There are about 15 species of scorpions in my country, such as East Asian pincer scorpion, mountain scorpion, Tibetan scorpion, spot scorpion, Liaoker scorpion, ten-legged scorpion, etc., and their distribution is also very wide, mainly distributed in the northern taboo between 32-42 degrees The vast areas of North China, Northeast China and the south of the Yangtze River, while the northwest inland with less water is less distributed. The East Asian scorpion, also known as the Martensii scorpion, is the most widely distributed in my count...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/34A61K35/646A61P35/00A61P29/00A61P25/06A61P7/02
CPCA61K35/646A61P7/02A61P25/06A61P29/00A61P35/00C07K1/34C07K14/43522
Inventor 金韶京
Owner 金华市飞凌生物科技有限公司