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A kind of composite protective agent for low-temperature freeze-drying of probiotics and its application

A low-temperature freeze-drying and protective agent technology, which is applied to the preservation of microorganisms, can solve the problems of increased cell membrane permeability, cell metabolism damage, etc., and achieve the effects of clear components, improved survival rate, and a wide range of applications

Active Publication Date: 2020-11-20
广州富诺营养科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the solute effect caused by freeze-drying will cause the cell water to overflow, the enzyme protein and inhibitory factor with biological activity will be denatured and inactivated, and the membrane permeability of the cell membrane will increase, so that the intracellular substance and the extracellular water-soluble substance will exchange bidirectionally without control. resulting in cellular metabolic damage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Composite protective agent for low-temperature freeze-drying of probiotics of the present invention and freeze-drying process thereof

[0019] A compound protective agent for low-temperature freeze-drying of probiotics, which is composed of the following components in weight percentage: 3.5% rhamnolipid, 1.5% malondialdehyde, 1.0% glutamic acid, 0.8% tyrosine, 7 -Decene-4-lactone 2.0%, disodium hydrogen phosphate 0.8%, sodium dihydrogen phosphate 0.8%, vitamin C 0.3%, the balance is distilled water.

[0020] The freeze-drying process includes the following steps: centrifuge the probiotic carrier at a centrifugation speed of 8000 rpm and a centrifugation time of 10 minutes to obtain the probiotic solid, mix the freeze-drying composite protective agent with the probiotic solid, and put Put it into a vacuum freeze dryer for freeze-drying, drop the temperature to -20°C at a cooling rate of 10°C every half hour, maintain the vacuum degree at 20pa for 4 hours, and r...

Embodiment 2

[0021] Example 2 Composite protective agent for low-temperature freeze-drying of probiotics of the present invention and its freeze-drying process

[0022] A compound protective agent for low-temperature freeze-drying of probiotics, which is composed of the following components in weight percentage: 5.5% rhamnolipid, 2.2% malondialdehyde, 1.6% glutamic acid, 1.2% tyrosine, 7 -Decene-4-lactone 4.0%, disodium hydrogen phosphate 1.5%, sodium dihydrogen phosphate 1.5%, vitamin C 0.5%, the balance is distilled water.

[0023] The freeze-drying process includes the following steps: centrifuge the probiotic carrier at a centrifugation speed of 10,000 rpm and a centrifugation time of 20 minutes to obtain a probiotic solid, mix the freeze-drying composite protective agent with the probiotic solid, and put Put it into a vacuum freeze dryer for freeze-drying, drop the temperature to -60°C at a rate of 10°C every half hour, maintain the vacuum at 40pa for 8 hours, and raise the temperatur...

Embodiment 3

[0024] Example 3 Composite protective agent for low-temperature freeze-drying of probiotics of the present invention and its freeze-drying process

[0025] A compound protective agent for low-temperature freeze-drying of probiotics, which is composed of the following components in weight percentage: 4.5% rhamnolipid, 1.8% malondialdehyde, 1.3% glutamic acid, 1.0% tyrosine, 7 -Decene-4-lactone 2.5%, disodium hydrogen phosphate 1.2%, sodium dihydrogen phosphate 1.2%, vitamin C 0.4%, the balance is distilled water.

[0026] The freeze-drying process includes the following steps: centrifuge the probiotic carrier at a centrifugation speed of 8000 rpm and a centrifugation time of 20 minutes to obtain the probiotic solid, mix the freeze-drying composite protective agent with the probiotic solid, and put Put it into a vacuum freeze dryer for freeze-drying, drop the temperature to -20°C at a cooling rate of 10°C every half hour, maintain the vacuum at 40pa for 4 hours, and raise the te...

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Abstract

The invention relates to a composite protective agent for low-temperature freeze-drying of probiotics and application thereof, and belong to the field of preparation of biological products, which aimsat improving the survival rate of the probiotics cells in a freeze-drying technology. The composite protective agent for the low-temperature freeze-drying of the probiotics is prepared from the following components in percentage by weight: 3.5 to 5.5% of rhamnolipid, 1.5 to 2.2% of malondialdehyde, 1.0 to 1.6% of glutamate, 0.8 to 1.2% of tyrosine, 2.0 to 4.0% of 7-decene-4-lactone, 0.8 to 1.5% of disodium hydrogen phosphate, 0.8 to 1.5% of sodium dihydrogen phosphate, 0.3 to 0.5% of vitamin C, and the balance of distilled water. After the composite protective agent is mixed with solid matters of the probiotics, the survival rate of the probiotics is greatly improved, and the application prospect is broad.

Description

technical field [0001] The invention relates to a composite protective agent for low-temperature freeze-drying of probiotics and an application thereof, belonging to the field of biological product preparation. Background technique [0002] Freeze-drying technology is to freeze the wet material or solution into a solid state at a lower temperature (-10--50°C), and then sublimate the water in it directly into a gaseous state without going through a liquid state under vacuum (1.3-13Pa), and finally make it Drying technology for material dehydration. The drying of the material is completed in a frozen state. Compared with other drying methods, the physical structure and molecular structure of the material have minimal changes, and its organizational structure and appearance are well preserved, and it has excellent rehydration properties. return to its pre-dry state. Since the drying process is carried out at a very low temperature and the air is basically isolated, it effecti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04
CPCC12N1/04
Inventor 程彦
Owner 广州富诺营养科技有限公司
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