Method of biosynthesizing nanometer tellurium by utilizing bacillus licheniformis and application of method
A Bacillus licheniformis, biosynthetic technology, applied in microorganism-based methods, biochemical equipment and methods, nanotechnology for materials and surface science, etc., to achieve a wide range of applications, avoid secondary pollution, and good biosafety Effect
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Embodiment 1
[0050] Embodiment 1 Bacillus licheniformis S13 is to the tolerance concentration of tellurite
[0051] 1. Prepare solid LB with different concentrations of tellurium-containing medium (each liter of medium contains 10g of NaCl, 10g of tryptone, 5g of yeast extract, 15g of agar, and 1L of deionized water), and autoclave at 121°C for 20 minutes; prepare tellurite The mother liquor (0.2M) was sterilized by filtration, and a tellurite solution was added to make the contents of tellurite in the medium respectively 0.05mM, 0.1mM, 0.2mM, 0.3mM, 0.4mM, 0.5mM, and 0.6mM.
[0052] 2. Pick a single colony of the S13 strain and inoculate it in LB liquid medium for shaking culture for 12 hours (150rpm, 37°C) to take the above bacterial solution and dilute it to OD 600 =0.8 mother solution for later use; drop 100 μL of the mother solution on plates containing different tellurium concentrations and spread evenly on the plate, each concentration has 6 replicates, culture at 37°C for 48 hours, a...
Embodiment 2
[0054] Example 2 The synthetic efficiency of Bacillus licheniformis S13 to biological nanometer tellurium under different tellurite concentrations
[0055] 1. Preparation of tellurite-containing liquid LB culture medium with different concentrations: prepare the mother liquor of acidite (0.2mM), filter and sterilize; add tellurite solution to the medium, so that the content of tellurite in the medium is 0.05mM, 0.1mM, 0.2mM, three repetitions of each concentration gradient, autoclaved at 121°C for 20min.
[0056] 2. Strain activation: pick a single colony of the S13 strain and inoculate it in LB liquid medium for 12 hours (150rpm, 37°C), then take the above bacterial solution and dilute to OD 600 =0.8; the diluted bacterial solution was inoculated in the above-mentioned tellurium-containing LB medium according to the inoculum amount of 0.1v / v%, and shaken for 72h (150-180rpm, 37°C).
[0057] 3. Determination of tellurite content: fully suspend the S13 bacterial liquid after 4...
Embodiment 3
[0061] The optimal culture time of embodiment 3 bacillus licheniformis S13 synthetic nano-tellurium
[0062] 1. Culture medium preparation and strain activation Refer to steps 1 and 2 in Example 1.
[0063] 2. Adjust the concentration of the activated S13 strain to OD 600 =0.8; the diluted bacterial solution was inoculated in 0.2mM tellurite-containing LB medium according to the inoculum amount of 0.1v / v%, shaken at 37°C and 150rpm, and the control and treatment were replicated 3 times. Samples were taken at 8h, 16h, 24h, 32h, 40h, 48h, 72h, 96h, 120h, and 144h of shaking culture, and each treatment was repeated three times.
[0064] 3. Centrifuge the cultured bacterial solution at 10000rprn for 10min, take 1mL of the supernatant, add 3mL of 0.5M Tris-HCl (pH7.0) solution and 1mL of 10mM DDTC solution, mix well, and measure the absorbance at a wavelength of 340nm within 5min.
[0065] 4. According to the standard curve conversion of tellurite absorbance, the content of tellu...
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