Polysubstituted-indazole compounds and application of same as IDO inhibitors

A compound and mixture technology, applied in the field of IDO inhibitor, can solve the problems of reducing tryptophan concentration, stagnation of synthesis, inhibiting killing effect, etc., and achieving excellent inhibitory effect.

Active Publication Date: 2018-10-23
XIHUA UNIV
View PDF6 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under normal circumstances, IDO is expressed at a low level in the body, but most tumor cells will form a high expression of IDO, which converts L-tryptophan into N-formylkynurenine, reducing the color of the cell microenvironment. The concentration of tryptophan makes the synthesis of tryptophan-dependent T cells stagnate in the G1 phase, and the proliferation of T cells is inhibited, thereby inhibiting the killing effect of the body's immune system on tumor tissue

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polysubstituted-indazole compounds and application of same as IDO inhibitors
  • Polysubstituted-indazole compounds and application of same as IDO inhibitors
  • Polysubstituted-indazole compounds and application of same as IDO inhibitors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Example 1 Synthesis of Compounds LWQ-136, LWQ-138, LWQ-156, LWQ-167, LWQ-223

[0072] The synthetic route is as follows:

[0073]

[0074] 1 Synthesis of raw material LWQ-221:

[0075] Take a dry 50mL pear-shaped bottle, add 2-methyl-1,3-dinitrobenzene (CAS: 606-20-2, 5.00g, 27.45mmol, purchased from Chengdu Ruioke Reagent Company) with 30mL concentrated sulfuric acid Dissolve, slowly add 1,3-dibromo-5,5-dimethylhydantoin (CAS: 77-48-5, 4.29g, 15.00mmol, purchased from Chengdu Ruioke Reagent Co., Ltd.) under stirring in an ice bath, drop After the addition was completed, the reaction was stirred at room temperature for 15 hours. TLC showed that the reaction of the raw materials was complete. The reaction solution was slowly poured into ice water, filtered, and the filter cake was vacuum-dried to obtain white solid powder 5-bromo-2methyl-1,3-dinitrobenzene (6.59 g, yield 92%).

[0076] Take a dry 50mL pear-shaped flask, dissolve 5-bromo-2methyl-1,3-dinitrobenzene (...

Embodiment 2

[0094] Example 2 Synthesis of Compounds LWQ-154, LWQ-155, LWQ-158, LWQ-159, LWQ-165, LWQ-166

[0095] The synthetic route is as follows:

[0096]

[0097] 1 Synthesis of intermediate 37b

[0098] Under the protection of Ar gas, triphenylphosphine methyl bromide (5.34 g, 15.06 mmol) was dissolved in redistilled THF and cooled to -78°C. Slowly add 2.5M n-butyllithium (6.00mL, 15.06mmol) dropwise, after the addition is complete, stir at the same temperature for 1h, add a THF solution of compound 36 (2.00g, 10.04mmol), stir for 1h, and gradually rise to 0°C. Add saturated ammonium chloride aqueous solution to stop the reaction, spin the reaction solution to dryness, add water to dilute the residue, extract with EA three times, wash with saturated sodium chloride aqueous solution twice, dry, and concentrate. The crude product was purified by column chromatography (PE:EA=20:1) to obtain compound 37b (600 g, 3.04 mmol) as a colorless oily liquid with a yield of 30%.

[0099] 2 ...

Embodiment 3

[0114] The synthesis of embodiment 3 compound LWQ-162, LWQ-163

[0115] The synthetic route is as follows:

[0116]

[0117] 1 Preparation of intermediate 40

[0118] Dissolve 3-azetidinecarboxylic acid 39 (2.47g, 24.46mmol) in 30mL methanol, slowly add SOCl dropwise at 0°C 2 (8.87 mL, 122.3 mmol). After addition, warm to room temperature and stir for 10h. The reaction solution was spin-dried, and the SOCl in the residue was removed with dichloromethane. 2 , and spun 3 times, the crude product was a colorless viscous liquid. Because the water solubility of compound 40 was very good, no further post-treatment was carried out, and the yield was quantified.

[0119] 2 Preparation of intermediate 41

[0120] Compound 40 (3.68g, 24.46mmol) was dissolved in acetonitrile, followed by adding Et 3 N (3.39mL, 24.46mmol), (Boc) 2 O (5.87 g, 26.91 mmol) in acetonitrile and DMAP (0.30 g, 2.45 mmol). After addition, stir at room temperature for 5h. TLC detected that the reaction...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses polysubstituted-indazole compounds as shown in a formula (I) which is described in the specification, a preparation method for the compounds, and application of the compounds as IDO inhibitors. The compounds provided by the invention can be used for preventing and / or treating a plurality of diseases, such as Alzheimer's disease, cataract, infections related to cellular immune activation, autoimmune diseases, AIDS, cancers, depression, the metabolic disorder of tryptophan or the like.

Description

technical field [0001] The present invention relates to indazole compound, and also relates to its preparation method and application as IDO inhibitor. Background technique [0002] Indoleamine 2,3-dioxygenase (Indoleamine 2,3-dioxygenase, IDO) catalyzes the epoxidation and cleavage of indole in indoleamine molecules such as tryptophan, making it catabolized by the kynuric acid pathway. fast enzyme. [0003] IDO plays an important role in tumor immune immunity and tumorigenesis. Under normal circumstances, IDO is expressed at a low level in the body, but most tumor cells will form a high expression of IDO, which converts L-tryptophan into N-formylkynurenine, reducing the color of the cell microenvironment. The concentration of tryptophan makes the synthesis of tryptophan-dependent T cells stagnate in the G1 phase, and the proliferation of T cells is inhibited, thereby inhibiting the killing effect of the body's immune system on tumor tissues. At the same time, the metabol...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07D231/56C07D403/12C07D405/12A61K31/416A61P25/28A61P27/12A61P37/02A61P31/18A61P35/00A61P25/24A61P3/00A61P31/00
CPCC07D231/56C07D403/12C07D405/12
Inventor 钱珊李国菠陈杨李超张曼王周玉杨羚羚赖朋
Owner XIHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap