A method of strengthening denitrification bacteria denitrification by using filamentous fungi as a carrier
A technology for denitrifying bacteria and denitrification, applied in chemical instruments and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of low success rate and long cultivation time of mixed mycelium balls, and achieve recycling and utilization. High rate, high reusability, and pollution reduction effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0052] (1) Enrichment culture of denitrifying bacteria: Take some sludge from the anoxic pool of the sewage treatment plant. Wash it with phosphate buffer (pH=7) and pass through a 200-mesh sieve to remove excess impurities. Dilute activated sludge and distilled water at a volume ratio of 1:4. After standing still for 1 hour, take 10ml of the mud-water mixture in the middle layer, put it in a conical flask, add 90ml of denitrification culture solution (Table 1 and Table 2), and feed argon to remove oxygen to ensure anaerobic conditions. When the nitrate concentration of the culture solution is reduced to a certain level, the mixed solution is taken and centrifuged at 4000 r / min for 15 minutes to obtain denitrifying bacteria. The same method was repeated three times to obtain denitrifying bacteria with relatively high abundance.
[0053] (2) Preparation of the denitrifying bacteria suspension: resuspend 1 g of the denitrifying bacteria sediment in (1) with 100 ml of sterilize...
Embodiment 2
[0071] (1) Enrichment culture of denitrifying bacteria: Take some sludge from the anoxic pool of the sewage treatment plant. Wash it with phosphate buffer (pH=7) and pass through a 200-mesh sieve to remove excess impurities. Dilute activated sludge and distilled water at a volume ratio of 1:4. After standing still for 1 hour, take 10ml of the mud-water mixture in the middle layer, put it in a conical flask, add 90ml of denitrification culture solution, and pass in argon to remove oxygen to ensure anaerobic conditions. When the nitrate concentration of the culture solution is reduced to a certain level, the mixed solution is taken and centrifuged at 4000 r / min for 15 minutes to obtain denitrifying bacteria. The same method was repeated three times to obtain denitrifying bacteria with relatively high abundance.
[0072] (2) Preparation of the denitrifying bacteria suspension: resuspend 1 g of the denitrifying bacteria sediment in (1) with 100 ml of sterilized water to obtain t...
PUM
| Property | Measurement | Unit |
|---|---|---|
| clearance rate | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More