Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for efficiently inducing lily polyploidy

A polyploid, lily technology, applied in the fields of botanical equipment and methods, plant genetic improvement, application, etc., can solve the problems of strict operating conditions, easy occurrence of chimeras, cumbersome and time-consuming processes, etc., to achieve short processing time, avoid The effect of easy pollution and cost reduction

Active Publication Date: 2020-06-30
YANGTZE NORMAL UNIVERSITY
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a method for efficiently inducing polyploidy in lilies, which mainly solves the cumbersome and time-consuming process of the polyploidy induction method in lily, strict operating conditions, high technical requirements, and the Large consumption, prone to chimera and other technical problems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for efficiently inducing lily polyploidy
  • A method for efficiently inducing lily polyploidy
  • A method for efficiently inducing lily polyploidy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1) Budding induction

[0043]Select an appropriate amount of bulbils of Chongqing Xiushan Juandan lily, take the outer and middle layer of bulbil scales, wash them with tap water, mix them in peat, wrap them in black plastic bags, and cultivate them for 1 to 2 weeks. During this period, the water content of peat is controlled at 15 Between ~25%, when the scales germinate, and the diameter of the buds is less than or equal to 1mm, wash them with tap water for 3 to 5 times to obtain the germinated scales. The above-mentioned germinated scales are divided into 12 groups on average according to the number of buds, and the number of buds in each group is 50. and put them into clean culture bottles;

[0044] 2) Preparation of colchicine solution

[0045] Colchicine is dissolved with 2% dimethyl sulfoxide to prepare solutions with mass percentages of 0.1%, 0.15% and 2%, respectively, and sterilized by high pressure to obtain 0.1%, 0.15% and 0.2% colchicine prime solution;

...

Embodiment 2

[0057] 1) Budding induction

[0058] Select an appropriate amount of Chongqing Xiushan Juandan lily bulbs, take the outer and middle scales, rinse with tap water, cut each scale into several small openings, and put them into a light substrate with a weight ratio of peat and wood chips of 3:1 Mix well, wrap in a black plastic bag, and cultivate for 1-2 weeks. During this period, control the water content of the light substrate between 15-25%. After the scales germinate, and the diameter of the buds is ≤1mm, rinse with tap water 3-5 times , to obtain budding scales, divide the above-mentioned budding scales into 12 groups on average according to the number of buds, and the number of buds in each group is 50, and put them into clean culture bottles respectively;

[0059] 2) Preparation of colchicine solution

[0060] Colchicine is dissolved with 2% dimethyl sulfoxide to prepare solutions with mass percentages of 0.1%, 0.15% and 2%, respectively, and sterilized by high pressure t...

Embodiment 3

[0077] 1) Budding induction

[0078] Select an appropriate amount of Lanzhou lily bulbs, take the outer and middle scales, wash them with tap water, put them into a light substrate with a weight ratio of peat and sawdust at a ratio of 3:1, and wrap them in black plastic bags, and cultivate them for 1 to 2 weeks. During this period, the water content of the light substrate is controlled between 15% and 25%. After the scales germinate and the diameter of the buds is ≤ 1 mm, they are washed with tap water for 3 to 5 times to obtain the germinated scales. Group, each group of buds is 50, and put into clean culture bottles respectively;

[0079] 2) Preparation of colchicine solution

[0080] Colchicine is dissolved with 2% dimethyl sulfoxide to prepare solutions with mass percentages of 0.1%, 0.15% and 2%, respectively, and sterilized by high pressure to obtain 0.1%, 0.15% and 0.2% colchicine prime solution;

[0081] 3) Polyploid induction

[0082] Add colchicine solutions of d...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for efficiently inducing lily polyploidy. The method includes the steps: 1) germination induction; 2) colchicine solution preparation; 3) polyploidy induction; 4) rooting culture; 5) ploidy identification. The method is simple, easy to implement, short in treatment time and high in induction rate, a bud of a lily grown in a light substrate is induced, and the lilypolyploidy can be induced. Compared with an existing universal inducing method, tissue culture and sterile operation are omitted, cost is reduced, the method avoids the problems that pollution is easily generated, operating conditions are strict, processes are complicated, time is consumed in the inducing process of common polyploidy, and the method is convenient to popularize and apply, can serveas an important method for cultivating new germplasm of the lily and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of polyploid breeding of lilies, and in particular relates to an efficient method for inducing polyploids of lilies. Background technique [0002] Lily is a perennial herbaceous bulbous plant of the genus Lilium in the family Liliaceae, and is currently mainly distributed in temperate regions of the northern hemisphere. There are more than 100 species of lily plants in the world, and about 55 species are native to my country. It has a long history of planting, a wide range of planting, and rich germplasm resources. Lily has large scales, thick flesh, little fiber, pure and sweet taste, and contains a lot of reducing sugar, protein, fat, starch and other nutrients needed by the human body, and has high nutritional value. In addition, Lanzhou lily and Lily lily contain not only common nutrients, but also 8 kinds of amino acids, vitamins, mineral elements, lilyside, colchicine and other medicinal ingredients ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08A01G22/60
CPCA01G22/60A01H1/08
Inventor 符勇耀杨利平
Owner YANGTZE NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com