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Feed additive for preventing and treating diarrhea of piglets

A feed additive, piglet diarrhea technology, applied in the direction of animal feed, animal feed, plant raw materials, etc., can solve the problems of less microbial lysozyme, limited application, limited application scope, etc., and achieve good results.

Active Publication Date: 2018-11-02
ZHEJIANG AEGIS BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the most common lysozyme on the market is egg white lysozyme, but it only has an effect on Gram-positive bacteria, which limits its application range
In recent years, extensive research on lysozyme from various sources has been carried out at home and abroad, but there are few research reports on the preparation of microbial lysozyme by fermentation methods, resulting in limited application of this type of non-egg white lysozyme in actual industrial production

Method used

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  • Feed additive for preventing and treating diarrhea of piglets
  • Feed additive for preventing and treating diarrhea of piglets
  • Feed additive for preventing and treating diarrhea of piglets

Examples

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Embodiment 1

[0029] Prepare liquid culture medium, wherein glucose 10g / L, peptone 5g / L. Get 25mL liquid culture medium and place in the Erlenmeyer flask, insert the thalline seed of Lysobacterium S2-6b according to the inoculum size of 4% (v / v), carry out shake flask fermentation, fermentation condition is 30 ℃, 200r / min, fermentation After cultivating for 24 hours, centrifuge the fermentation broth to remove the bacteria, take the supernatant and use ammonium sulfate salting-out method (add ammonium sulfate to the supernatant to 20% saturation, stir for 1 hour, let it stand for 1 hour, centrifuge at 10000r / min, and use it for precipitation) Dissolve in distilled water, put it into a dialysis bag with a molecular weight of 10,000, and place it in 10mmol / L PBS for full dialysis; add ammonium sulfate to the supernatant in the dialysis bag to 70% saturation, stir for 1 hour, let it stand for 1 hour, and centrifuge at 10,000r / min. The precipitate was dialyzed in the same way as above to obtain...

Embodiment 2

[0031] Prepare liquid culture medium, wherein glucose 10g / L, peptone 5g / L. Get 25mL liquid culture medium and place in the Erlenmeyer flask, insert the thalline seed of Lysobacterium S2-6b according to the inoculum amount of 4% (v / v), carry out shake flask fermentation, fermentation condition is 20 ℃, 150r / min, fermentation After cultivating for 24 hours, centrifuge the fermentation broth to remove the bacteria, take the supernatant and use ammonium sulfate salting-out method (add ammonium sulfate to the supernatant to 20% saturation, stir for 1 hour, let it stand for 1 hour, centrifuge at 10000r / min, and use it for precipitation) Dissolve in distilled water, put it into a dialysis bag with a molecular weight of 10,000, and place it in 10mmol / L PBS for full dialysis; add ammonium sulfate to the supernatant in the dialysis bag to 70% saturation, stir for 1 hour, let it stand for 1 hour, and centrifuge at 10,000r / min. Take the precipitate and dialyze, the method is the same as a...

Embodiment 3

[0033]Prepare liquid culture medium, wherein glucose 10g / L, peptone 5g / L. Get 25mL of liquid culture medium and place it in the Erlenmeyer flask, insert the thalline seed of Lysobacterium S2-6b according to the inoculum size of 4% (v / v), carry out shake flask fermentation, fermentation condition is 40 ℃, 300r / min, fermentation After cultivating for 24 hours, centrifuge the fermentation broth to remove the bacteria, take the supernatant and use ammonium sulfate salting-out method (add ammonium sulfate to the supernatant to 20% saturation, stir for 1 hour, let it stand for 1 hour, centrifuge at 10000r / min, and use it for precipitation) Dissolve in distilled water, put it into a dialysis bag with a molecular weight of 10,000, and place it in 10mmol / L PBS for full dialysis; add ammonium sulfate to the supernatant in the dialysis bag to 70% saturation, stir for 1 hour, let it stand for 1 hour, and centrifuge at 10,000r / min. The precipitate was dialyzed, and the dialysate was obtain...

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Abstract

The invention relates to a feed additive containing lysozyme. The lysozyme is prepared by a fermentation process which comprises the following specific steps: placing a liquid culture medium in a triangular flask; inoculating the medium with lysobacter strains in an inoculation amount of 4% v / v; carrying out flask-shaking fermentation, wherein the fermentation conditions are 20-40 DEG C and 150-300 r / min; after 24 hours of the fermentation, centrifuging fermentation liquor to remove thalli; purifying the obtained supernatant by successively using an ammonium sulfate salting-out method, a gel-filtration chromatography and a positive ion-exchange chromatography method; and conducting freeze drying. The lysozyme feed additive obtained by using the fermentation process has good effects of preventing and treating diarrhea of piglets, and has better effect when cooperating with traditional Chinese medicine extract.

Description

technical field [0001] The invention relates to the field of feed, in particular to a feed additive for preventing and treating piglet diarrhea. Background technique [0002] In recent years, with the highly intensive production of the animal husbandry industry, the incidence of livestock and poultry diseases has also increased day by day, and the mortality rate due to diseases has increased year by year, causing major economic losses to the animal husbandry industry. Diarrhea is one of the main factors affecting the survival rate and growth of piglets. The traditional prevention and treatment measures are the use of antibiotics. Although the use of antibiotics in livestock and poultry feed has played an important role in effectively controlling diseases, improving feed conversion rate, and ensuring the stable supply of livestock products, due to the over-range, high-dose or multi-species, low-dose antibiotics in feed production for a long time The use, as well as the abuse...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/30A23K20/189A23K10/30
CPCA23K50/30A23K50/60A23K20/189A23K10/30A61K36/756A61K38/47C12Y302/01017A61P1/12A61K2300/00Y02P60/87
Inventor 潘宏涛卢亚萍
Owner ZHEJIANG AEGIS BIOLOGICAL SCI & TECH CO LTD
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